ANTIBACTERIAL, ANTIOXIDANT, CHEMICAL CONSTITUENTS, AND CYTOTOXICITY EVALUATION OF TERMINALIA ARJUNA (ROXB. EX DC.) WIGHT AND ARN


Savita Khatri, Neetu Phougat, Mehak Dangi, Anil Kumar Chhillar

Abstract


ABSTRACT
Objective: The objective of this study is to evaluate the in vitro antibacterial and antioxidant prospective of Terminalia arjuna (leaves). The most active
extracts were examined for their chemical composition and cytotoxicity.
Methods: The antibacterial activity of five different extracts were examined against 8 bacterial strains (5 Gram-positive and 3 Gram-negative) using
resazurin-based microtiter dilution assay (RMDA) and disk-diffusion assay. The antioxidant potential of five extracts was demonstrated using 1,
1-diphenyl-2-picrylhydrazyl (DPPH) assay and superoxide radical scavenging assay. Chemical composition and cytotoxicity were assessed using gas
chromatography-mass spectrometry (GC-MS) and hemolytic assay, respectively.
Results: According to RMDA, the acetone extract (AE) exhibited highest antibacterial activity. The AE showed highest activity against Salmonella
enterica ser. typhi and Bacillus cereus with minimum inhibitory concentration, i.e., 195.31 μg/ml. In DPPH assay, AE showed the highest radical
scavenging activity with inhibition concentration50 23.09 μg/ml. In GC-MS analysis, the principal compound in AE was celidoniol (8.72 %). According
to the results of hemolytic assay, the AE showed non-toxic behavior upto 500 μg/ml.
Conclusion: The present investigation represents T. arjuna as an incredible herb. The AE was found to possess promising antibacterial and antioxidant
properties.
Keywords: Antibacterial, Antioxidant, Terminalia arjuna, Chemical composition, Cytotoxicity.


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Table 3: Phytoconstituents in AE of T. arjuna (leaves) in GCMS analysis

S.N

R time

Compound

% Area

Molecular formula (Mol. wt.)

Nature of compound

Chemical structure

069

, 6, 10‑trimethyl, 14‑ethylene‑14‑pentadecane

04

C20H38

(278)

Olefins

551

Heneicosane

02

C21H44

(296)

N‑Alkanes

842

Eicosane

82

C20H42

(282)

N‑Alkanes

867

Dotriacontane

50

C32H66

(450)

N‑Alkanes

896

Tetratriacontane

07

C34H70

(478)

N‑Alkanes

136

Pentacosane

58

C25H52

(352)

N‑Alkanes

663

Celidoniol

72

C29H60

(408)

N‑Alkanes

597

Hexacosane

15

C26H54

(366)

N‑Alkanes

703

Stigmast‑5‑en‑3‑ol

58

C29H50O

(414)

Steroid compound

917

Oxirane

04

C18H36O

(268)

Cyclic ether

AE: Acetone extract, T. arjuna: Terminalia arjuna

Asian J Pharm Clin Res, Vol 10, Issue 3, 2017, 412-417

Khatri et al.

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About this article

Title

ANTIBACTERIAL, ANTIOXIDANT, CHEMICAL CONSTITUENTS, AND CYTOTOXICITY EVALUATION OF TERMINALIA ARJUNA (ROXB. EX DC.) WIGHT AND ARN

DOI

10.22159/ajpcr.2017.v10i3.16408

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 412-417

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

159 Views | 156 Downloads

Authors & Affiliations

Savita Khatri
India

Neetu Phougat

Mehak Dangi

Anil Kumar Chhillar


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