ANTIBACTERIAL, ANTIOXIDANT, CHEMICAL CONSTITUENTS, AND CYTOTOXICITY EVALUATION OF TERMINALIA ARJUNA (ROXB. EX DC.) WIGHT AND ARN

  • Savita Khatri
  • Neetu Phougat
  • Mehak Dangi
  • Anil Kumar Chhillar

Abstract

ABSTRACT
Objective: The objective of this study is to evaluate the in vitro antibacterial and antioxidant prospective of Terminalia arjuna (leaves). The most active
extracts were examined for their chemical composition and cytotoxicity.
Methods: The antibacterial activity of five different extracts were examined against 8 bacterial strains (5 Gram-positive and 3 Gram-negative) using
resazurin-based microtiter dilution assay (RMDA) and disk-diffusion assay. The antioxidant potential of five extracts was demonstrated using 1,
1-diphenyl-2-picrylhydrazyl (DPPH) assay and superoxide radical scavenging assay. Chemical composition and cytotoxicity were assessed using gas
chromatography-mass spectrometry (GC-MS) and hemolytic assay, respectively.
Results: According to RMDA, the acetone extract (AE) exhibited highest antibacterial activity. The AE showed highest activity against Salmonella
enterica ser. typhi and Bacillus cereus with minimum inhibitory concentration, i.e., 195.31 μg/ml. In DPPH assay, AE showed the highest radical
scavenging activity with inhibition concentration50 23.09 μg/ml. In GC-MS analysis, the principal compound in AE was celidoniol (8.72 %). According
to the results of hemolytic assay, the AE showed non-toxic behavior upto 500 μg/ml.
Conclusion: The present investigation represents T. arjuna as an incredible herb. The AE was found to possess promising antibacterial and antioxidant
properties.
Keywords: Antibacterial, Antioxidant, Terminalia arjuna, Chemical composition, Cytotoxicity.

References

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Table 3: Phytoconstituents in AE of T. arjuna (leaves) in GCMS analysis
S.N
R time
Compound
% Area
Molecular formula (Mol. wt.)
Nature of compound
Chemical structure
1
13.069
2, 6, 10‑trimethyl, 14‑ethylene‑14‑pentadecane
3.04
C20H38
(278)
Olefins
2
19.551
Heneicosane
3.02
C21H44
(296)
N‑Alkanes
3
20.842
Eicosane
3.82
C20H42
(282)
N‑Alkanes
4
21.867
Dotriacontane
4.50
C32H66
(450)
N‑Alkanes
5
23.896
Tetratriacontane
6.07
C34H70
(478)
N‑Alkanes
6
25.136
Pentacosane
5.58
C25H52
(352)
N‑Alkanes
7
26.663
Celidoniol
8.72
C29H60
(408)
N‑Alkanes
8
30.597
Hexacosane
4.15
C26H54
(366)
N‑Alkanes
9
31.703
Stigmast‑5‑en‑3‑ol
5.58
C29H50O
(414)
Steroid compound
10
34.917
Oxirane
5.04
C18H36O
(268)
Cyclic ether
AE: Acetone extract, T. arjuna: Terminalia arjuna
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Khatri, S., N. Phougat, M. Dangi, and A. K. Chhillar. “ANTIBACTERIAL, ANTIOXIDANT, CHEMICAL CONSTITUENTS, AND CYTOTOXICITY EVALUATION OF TERMINALIA ARJUNA (ROXB. EX DC.) WIGHT AND ARN”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 3, Mar. 2017, pp. 412-7, doi:10.22159/ajpcr.2017.v10i3.16408.
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