IN VITRO ANTIMYCOTIC EVALUATION AND PRELIMINARY COMBINATORIAL STUDIES OF IBUPROFEN WITH STANDARD ANTIFUNGAL DRUGS AGAINST ASPERGILLUS SPP.


Mrridula Dangi Narwal, Meenakshi Balhara, Renu Chaudhary, Anil Kumar Chhillar

Abstract


Objective: The prevalence of invasive mycoses is increased in the immunocompromised patients with an increase in resistance developed againstcurrent antifungal drugs. This has led to the need for discovering novel combinations of the antifungal drugs to combat against resistant pathogenic spp.This study mainly targets to evaluate the antifungal activity of ibuprofen (IBU) alone and in combination with the standard antifungal drugs (polyenesand azoles) against eight isolates of Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger.

Methods: The study was performed using the disc diffusion assay (DDA), microbroth dilution assay and spore germination inhibition assay. Moreover,cytotoxicity was checked by heamolytic assay.

Results: Minimum inhibitory concentration (MIC) of IBU against A. fumigatus and A. flavus using DDA is found to be in the range of 250-275 μg/disc while for A. niger isolates, the range was 500-575 μg/disc. Likewise, by broth microdilution assay and spore germination inhibitory assay, MICdetermined, were in the range of 500-750 μg/ml against A. fumigatus and A. flavus while for A. niger, it was 1000-1500 μg/ml.

Conclusion: IBU demonstrated its antimycotic potential against all the eight isolates of Aspergillus spp. Moreover, preliminary combinatorialevaluation of IBU with the standard antifungal drugs reported by DDA revealed an increase in zone of inhibition as compared to the drugs alone.Further research regarding the confirmation of synergistic interaction between the selected drugs is in progress.


Keywords


Aspergillosis, Antifungal, Combination, Ibuprofen.

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About this article

Title

IN VITRO ANTIMYCOTIC EVALUATION AND PRELIMINARY COMBINATORIAL STUDIES OF IBUPROFEN WITH STANDARD ANTIFUNGAL DRUGS AGAINST ASPERGILLUS SPP.

Keywords

Aspergillosis, Antifungal, Combination, Ibuprofen.

DOI

10.22159/ajpcr.2017.v10i8.18188

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 8 August 2017 Page: 113-116

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

182 Views | 99 Downloads

Authors & Affiliations

Mrridula Dangi Narwal
Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryanan, India
India

Meenakshi Balhara
Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryanan, India
India

Renu Chaudhary
Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryanan, India
India

Anil Kumar Chhillar
Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryanan, India
India


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