ANTIFUNGAL POTENTIAL OF ETHANOL EXTRACTS OF ALLIUM SATIVUM AND ALLIUM AMPELOPRASUM


Shahid Khan, Neeta Raj Sharma

Abstract


Objective: In vitro analysis of Allium sativum and Allium ampeloprasum was performed to evaluate their antifungal potential against Alternaria triticina (ITCC 5496), causative agent of leaf blight in wheat and Magnaporthe oryzae (ITCC 6808), causative agent of blast disease in rice.

Methods: Ethanol extracts of A. ampeloprasum and A. sativum were prepared by crushing their bulb in liquid nitrogen and then immersing them in 90% ethanol and 100% ethanol separately. The antifungal activity test was determined by quantitative assay using 96-well microtiter plate and results were statistically analyzed using GraphPad Prism v. 5.03.

Results: A. triticina and M. oryzae showed above 90% and 95% growth inhibition, respectively against the ethanol extracts of A. ampeloprasum. Conversely, growth inhibition of either fungus remained mostly below 35% against ethanol extracts of A. sativum at all tested concentrations.

Conclusion: Ethanol extracts of A. ampeloprasum have relatively higher antifungal potential than ethanol extracts of A. sativum and could be considered as a natural alternative to chemical fungicides.

Keywords: Allium sativum, Allium ampeloprasum, Alternaria triticina, Magnaporthe oryzae.


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References


Block E. The chemistry of garlic and onions. Sci Am 1985;252(3):114-9.

Sharanappa R, Vidyasagar GM. Anti-Candida activity of medicinal plants: A review. Int J Pharm Pharm Sci 2013;5(4):9-16.

Shivakumar PS, Vidyasagar GM. Antifungal screening of 61 traditional medicinal plants of 305 extracts against dermatophytic fungi Trichophyton tonsurans. Int J Pharm Pharm Sci 2014;7(3):186-90.

Corzo-Martinez M, Corzo N, Villamiel M. Biological properties of onions and garlic. Trends Food Sci Technol 2007;18(12):609-25.

Yamada Y, Azuma K. Evaluation of the in vitro antifungal activity of allicin. Antimicrob Agents Chemother 1977;11(4):743-9.

Naganawa R, Iwata N, Ishikawa K, Fukuda H, Fujino T, Suzuki A. Inhibition of microbial growth by ajoene, a sulfur-containing compound derived from garlic. Appl Environ Microbiol 1996;62(11):4238-42.

Irkin R, Koruklouglu M. Control of Aspergillus niger with garlic, onion and leek extracts. Afr J Biotechnol 2007;6(4):384-7.

Morita T, Ushiroguchi T, Hayashi N, Matsuura H, Itakura Y, Fuwa T. Steroidal saponins from elephant garlic, bulbs of Allium ampeloprasum L. Chem Pharm Bull (Tokyo) 1988;36(9):3480-6.

Dey P, Khaled KL. An extensive review on Allium ampeloprasum a magical herb. Int J Sci Res 2013;4(7):371-7.

Kociã-Tanackov SD, Dimiã GR, Tepiã AN Vujiåiã BL. Influence of Allium ampeloprasum L. and Allium cepa L. Essential oils on the growth of some yeasts and moulds. Zb Matice Srp Prir Nauke 2009;116:121-30.

Sadeghi M, Zolfaghari B, Senatore M, Lanzotti V. Antifungal cinnamic acid derivatives from Persian leek (Allium ampeloprasum Subsp. Persicum). Phytochem Lett 2013;6(3):360-3.

Khudhair MW, Aboud HM, Dheyab NS, Shbar AK, Khalaf HS. The first record of Alternaria triticina the causative agent of Alternaria leaf blight in wheat and barley in Iraq. Int J Phytopathol 2014;3(3):133-8.

Singh RN, Singh AK, Singh SP, Singh BN. Prevalence and distribution of foliar blight pathogens attacking wheat in India. Indian Phytopathol 2001;54(2):175-8.

Dean R, VanKan JA, Pretorius ZA, Hammond-Kosack KE, Di Pietro A, Spanu PD, et al. The top 10 fungal pathogens in molecular plant pathology. Mol Plant Pathol 2012;13(7):414-30.

Gowda M, Shirke MD, Mahesha HB, Chandarana P, Rajamani A, Chattoo BB. Genome analysis of rice-blast fungus Magnaporthe oryzae field isolates from southern India. Genom Data 2015;5:284-91.

Perello A, Martinez I, Molina M. First report of virulence and effects of Magnaporthe oryzae isolates causing wheat blast in Argentina. Plant Dis 2015;99(8):1177-8.

Fakruddin M, Mannan KS, Mazumdar RM, Afroz H. Antibacterial, antifungal and antioxidant activities of the ethanol extract of the stem bark of Clausena heptaphylla. BMC Complement Altern Med 2012;12:232.

Zhang L, Ravipati AS, Koyyalamudi SR, Jeong SC, Reddy N, Bartlett J, et al. Anti-fungal and anti-bacterial activities of ethanol extracts of selected traditional Chinese medicinal herbs. Asian Pac J Trop Med 2013;6(9):673-81.

Rebaya A, Belghith SI, Hammrouni S, Maaroufi A, Ayadi MT, Chérif JK. Antibacterial and antifungal activities of ethanol extracts of Halimium halimifolium, Cistus salviifolius and Cistus monspeliensis. Int J Pharm Clin Res 2016;8(4):243-47.

Irkin R, Koruklouglu M. Control of some filamentous fungi and yeasts by dehydrated Allium extracts. J Verbrauch Lebensm 2009;4(1):3-6.

Akinmusire OO, Omomowo IO, Usman IM. Evaluation of the phytochemical properties and antifungal activities of ethanol extract of Allium sativum. Int J Curr Microbiol Appl Sci 2014;3(10):143-9.

Broekart WF, Terras RF, Cammune BP, Vanderleyden J. An automated quantitative assay for fungal growth inhibition. FEMS Microbiol Lett 1990;69(1-2):55-60.

Dellavale PD, Cabrera A, Alem D, Larranga P, Ferriera F, Rizza MD. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chillán En línea 2011;71(2):231-9.

Borlinghaus J, Albrecht F, Gruhlke MC, Nwachukwu ID, Slusarenko AJ. Allicin: Chemistry and biological properties. Molecules 2014;19:12591-618.

Focke M, Feld A, Lichtenthaler K. Allicin, a naturally occurring antibiotic from garlic, specifically inhibits acetyl-CoA synthetase. FEBS Lett 1990;261:106-8.

Ledezma E, Apitz-Castro R. Ajoene the main active compound of garlic (Allium sativum): A new antifungal agent. Rev Iberoam Micol 2006;23(2):75-80.

Yoshida S, Kasuga S, Hayashi N, Ushiroguchi T, Matsuura H, Nakagawa S. Antifungal activity of ajoene derived from garlic. Appl Environ Microbiol 1987;53(3):615-7.

Yin MC, Tsao SM. Inhibitory effect of seven Allium plants upon three Aspergillus species. Int J Food Microbiol 1999;49(1-2):49-56.

Lemar KM, Turner MP, Lloyd D. Garlic (Allium sativum) as an anti-candida agent: A comparison of the efficacy of fresh garlic and freeze-dried extracts. J Appl Microbiol 2002;93(3):398-405.

Perello A, Noll U, Slusarenko AJ. In vitro efficacy of garlic extract to control fungal pathogens of wheat. J Med Plants Res 2013;7(24):1809-17.

Masangwa JI, Aveling TA, Kritzinger Q. Screening of plant extracts for antifungal activities against Colletotrichum species of common bean (Phaseolus vulgaris L.) and cowpea (Vigna unguiculata (L.) Walp). J Agric Sci 2013;151(4):482-91.




About this article

Title

ANTIFUNGAL POTENTIAL OF ETHANOL EXTRACTS OF ALLIUM SATIVUM AND ALLIUM AMPELOPRASUM

DOI

10.22159/ajpcr.2017.v10i4.16555

Date

01-04-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 207-210

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

97 Views | 76 Downloads

Authors & Affiliations

Shahid Khan
Lovely Professional University
India

Neeta Raj Sharma


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