ANTIFUNGAL POTENTIAL OF ETHANOL EXTRACTS OF ALLIUM SATIVUM AND ALLIUM AMPELOPRASUM
Objective: In vitro analysis of Allium sativum and Allium ampeloprasum was performed to evaluate their antifungal potential against Alternaria triticina (ITCC 5496), causative agent of leaf blight in wheat and Magnaporthe oryzae (ITCC 6808), causative agent of blast disease in rice.
Methods: Ethanol extracts of A. ampeloprasum and A. sativum were prepared by crushing their bulb in liquid nitrogen and then immersing them in 90% ethanol and 100% ethanol separately. The antifungal activity test was determined by quantitative assay using 96-well microtiter plate and results were statistically analyzed using GraphPad Prism v. 5.03.
Results: A. triticina and M. oryzae showed above 90% and 95% growth inhibition, respectively against the ethanol extracts of A. ampeloprasum. Conversely, growth inhibition of either fungus remained mostly below 35% against ethanol extracts of A. sativum at all tested concentrations.
Conclusion: Ethanol extracts of A. ampeloprasum have relatively higher antifungal potential than ethanol extracts of A. sativum and could be considered as a natural alternative to chemical fungicides.
Keywords: Allium sativum, Allium ampeloprasum, Alternaria triticina, Magnaporthe oryzae.
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