BIOLOGICAL ACTIVITIES OF NOVEL IN VITRO RAISED STEVIA PLANT


Neeta Raj Sharma, Vineet Meshram, Mahiti Gupta

Abstract


 

 Objective: This communication explores a lead fraction from methanolic extract of novel Stevia species raised under in vitro conditions for its various biological activities.

Methods: The dried Stevia leaves were crushed in methanol to get the polar extract. This methanol extract was tested for pancreatic lipase and alpha-amylase inhibitory activity using quantitative plate assays. Antibacterial property of the extract was also evaluated against Staphylococcus epidermidis, Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa. Further, the antioxidant potential was evaluated using 1,1-diphenyl-2-picrylhydrazyl.

Results: The methanolic extract inhibited pancreatic lipase with IC50 of 5.74 μg/ml in a similar manner to a well-known anti-obesity drug in the market orlistat. The methanolic extract also showed a better pancreatic α-amylase inhibitory activity (IC50=88 μg/ml) than acarbose. Further, the lead fraction exhibited 88.48% antioxidant activity. It also exhibited broad spectrum antimicrobial activity against the spectrum of Gram-positive and Gram-negative bacteria tested under laboratory conditions with a minimal inhibitory concentration ranging from 1.95 to 31.25 μg/ml.

Conclusion: Thus, this study signifies the vast potential of the lead fraction from a novel Stevia species for further development into a herbal formulation for prevention of various infectious and non-infectious diseases.


Keywords


Antioxidants, Enzyme inhibitor, Minimal inhibitory concentration, Stevia.

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About this article

Title

BIOLOGICAL ACTIVITIES OF NOVEL IN VITRO RAISED STEVIA PLANT

Keywords

Antioxidants, Enzyme inhibitor, Minimal inhibitory concentration, Stevia.

DOI

10.22159/ajpcr.2017.v10i8.19117

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 8 August 2017 Page: 240-243

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

153 Views | 174 Downloads

Authors & Affiliations

Neeta Raj Sharma
Department of Biotechnology, Lovely Professional University, Phagwara, Punjab, India.
India

Vineet Meshram
Department of Biochemistry, DAV University, Jalandhar, Punjab, India.
India

Mahiti Gupta
Department of Biotechnology, Lovely Professional University, Phagwara, Punjab, India.
India


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