OXIDATIVE STRESS REDUCING AND LIVER PROTECTIVE EFFICACY OF ETHYL ACETATE FRACTION ISOLATED FROM PAJANELIA LONGIFOLIA (WILLD) K. SCHUMAN


Suparna Datta, Manabendra Dutta Choudhury

Abstract


Objectives: We investigated the liver protective as well as oxidative stress reducing activity of isolated fractions (Pajanelia fraction-1, 2, and 3 [PF1, PF2, and [PF3]) from ethyl acetate extract of Indian medicinal plant Pajanelia longifolia (Willd.) K. Schuman.

Methods: The liver protective activity of fractions was evaluated against carbon tetrachloride (CCl4) (0.5 ml/kg body weight intraperitoneal [b.w. i.p.]) induced hepatic damage in Swiss albino mice. Isolated fractions (at dose level of 150 mg/kg b.w. per orally [p.o.]) were administered to mice before 30 minutes of CCl4 administration. Silymarin at a dose of 50 mg/kg b.w. p.o. was taken as a standard drug. The liver protective activity of fractions was measured on serum biochemical parameters such as serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, serum alkaline phosphatase, and bilirubin (total). Further antioxidant activity of fractions was also measured on antioxidant enzymatic and non-enzymatic levels such as reduced glutathione, lipid peroxidation, superoxide dismutase, catalase, and glutathione peroxidase.

Results: The in vivo study revealed that the PF3 most effectively protected the liver from damage by CCl4. The findings were also confirmed by histopathological observations. High-performance liquid chromatography result was clearly indicated that the fraction was pure.

Conclution: Ethyl acetate fraction (Pajanelia fraction 3) from P. longifolia may be considered as a liver protective agent.


Keywords


Liver protective activity, Biochemical parameters, High-performance liquid chromatography study, Histopathological observations, Pajanelia longifolia (Willd.) K. Schuman

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About this article

Title

OXIDATIVE STRESS REDUCING AND LIVER PROTECTIVE EFFICACY OF ETHYL ACETATE FRACTION ISOLATED FROM PAJANELIA LONGIFOLIA (WILLD) K. SCHUMAN

Keywords

Liver protective activity, Biochemical parameters, High-performance liquid chromatography study, Histopathological observations, Pajanelia longifolia (Willd.) K. Schuman

DOI

10.22159/ajpcr.2017.v10i6.16898

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 95-99

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

125 Views | 156 Downloads

Authors & Affiliations

Suparna Datta
Ethnobotany and Medicinal plants Research Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, Assam, India
India

Manabendra Dutta Choudhury
Ethnobotany and Medicinal plants Research Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, Assam, India
India


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