Prevalence of various β-lactamase (ESBL, AmpC and MBL) producing multidrug resistant clinical isolates of Acinetobacter spp. in a tertiary care hospital.
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NilAbstract
Background: Extensive use of β- lactam group of antibiotics to treat the gram negative bacterial infections the antibiotic resistance are increasing and Acinetobacter species are budding as multidrug resistant (MDR) gram negative bacteria showing various β-lactamases mediated resistance.
Materials and Methods: The presence of β-lactamases in the multi drug resistant clinical isolates of Acinetobacter species were screened by β-lactamase assay method. The presence of ESBL was detected by phenotypic confirmatory test recommended by Clinical and Laboratory Standards Institute (CLSI), AmpC was detected by CX/BA inhibitor method and carbapenem (IMP or MER)/EDTA combined disc method and Modified Hodge tests were performed to detect MBL mediated resistance mechanism.
Results: We found high level of beta lactamase mediated resistance mechanism as part of multidrug resistance. Among 180 MDR isolates, 147 isolates were shown positive for beta lactamase assay method and these 147 isolates were further subjected to confirm presence of beta lactamase such as AmpC 105 (71.42%) followed by ESBL 84 (57.14%), MBL 43 (29.25%). Co-existence of ESBL and MBL also identified in 13 (8.84%) isolates.
Conclusion: β-lactamase mediated resistance mechanisms are accounting very high in the multidrug resistant isolates of Acinetobacter species. Therefore early detection is necessary to screen and confirm beta lactamase mediated resistant strains to avoid treatment failure and prevent the spread of MDR.
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