OCCURRENCE OF VARIOUS BETA-LACTAMASE PRODUCING GRAM NEGATIVE BACILLI IN THE HOSPITAL EFFLUENT.
AbstractThis study was designed to understand the prevalence of antibiotic resistant Gram negative bacilli producing various beta lactamases in hospital effluents. A total of 121 Gram negative bacilli were isolated and identified by standard biochemical tests from 31 hospital effluent samples. Antibiotic susceptibility profiling of isolated bacteria was assessed by Kirby Bauer disc method. Detection of various (ESBL, AmpC and MBL) producing isolates were further carried out by various methods. From the isolated bacteria, E.coli was predominant (37.19%) followed by Pseudomonas spp. (22.31%) Klebsiella spp. (19.83%) Non fermentative gram negative bacilli (NFGNB-10.74%), Enterobacter spp. (6.61%) and others (3.30 %). The present study suggests that although waste water treatment reduces the number of bacteria however, there is chance of antimicrobial resistant organisms in the hospital effluent. Hospitals should take sanitary measures to prevent the spread of multi drug resistant bacteria including beta-lactamases resistant strains transfer between hospital and the environment. The indiscriminate use of antibiotics in hospitals should be reduced.
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