EVALUATION OF IN VITRO- ANTI-OXIDANT POTENTIAL OF AQUEOUS ROOT EXTRACT OF CLERODENDRUM SERRATUM L.
Objective: The intent Â of this report Â was to investigate the effect of aqueous root extract of Clerodendrum serratum L. for antioxidant activity using divergent models viz. DPPH scavenging assay, Superoxide scavenging assay and Ferric Reducing Antioxidant Power (FRAP) assay.
Materials and Methods: The root of C. serratum was extracted using water. The yield of aqueous extract was 10%w/w. The outcome was examined statistically by the regression method.
Results and discussions: The IC50 values are 85.43 Âµg/ml and 107.59 Âµg/ml for DPPH radical scavenging and Superoxide scavenging assay respectively whereas Â FRAP showed significant reducing power activity with increased concentration of sample. The pilot study showed, a significant correlation existed between concentrations of the extract and percentage engrossment of free radicals.
Conclusion: The antioxidant property may be corresponding to the polyphenols and flavonoids adjacent in the extract. These results clearly revealed that C. serratum might be effective against diseases analogous with free radical mediated.
Keywords Clerodendrum serratum, DPPH, Superoxide, FRAP, Rutin, Antioxidant
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Fig. 3: Superoxide scavenging assay whereas % superoxide scavenging inhibition is plotted against concentrations of rutin
Fig. 4: Superoxide scavenging assay whereas % superoxide scavenging inhibition is plotted against concentrations of aqueous root extract
Fig. 5: Ferric reducing antioxidant power assay showing antioxidant capacity based on the ability to reduce ferric ions of sample with increase in absorbance of sample and standard
Table 2: Superoxide scavenging assay
IC50: Inhibitory concentration 50%
Table 3: FRAP assay
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