EVALUATION OF IN VITRO- ANTI-OXIDANT POTENTIAL OF AQUEOUS ROOT EXTRACT OF CLERODENDRUM SERRATUM L.


Raj Kumar Tiwari, Udayabanu Malairaman, Silpi Chanda

Abstract


Objective: The intent  of this report  was to investigate the effect of aqueous root extract of Clerodendrum serratum L. for antioxidant activity using divergent models viz. DPPH scavenging assay, Superoxide scavenging assay and Ferric Reducing Antioxidant Power (FRAP) assay.

Materials and Methods: The root of C. serratum was extracted using water. The yield of aqueous extract was 10%w/w. The outcome was examined statistically by the regression method.

Results and discussions: The IC50 values are 85.43 µg/ml and 107.59 µg/ml for DPPH radical scavenging and Superoxide scavenging assay respectively whereas  FRAP showed significant reducing power activity with increased concentration of sample. The pilot study showed, a significant correlation existed between concentrations of the extract and percentage engrossment of free radicals.

Conclusion: The antioxidant property may be corresponding to the polyphenols and flavonoids adjacent in the extract. These results clearly revealed that C. serratum might be effective against diseases analogous with free radical mediated.

 

Keywords Clerodendrum serratum, DPPH, Superoxide, FRAP, Rutin, Antioxidant


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Fig. 3: Superoxide scavenging assay whereas % superoxide scavenging inhibition is plotted against concentrations of rutin

Fig. 4: Superoxide scavenging assay whereas % superoxide scavenging inhibition is plotted against concentrations of aqueous root extract

Fig. 5: Ferric reducing antioxidant power assay showing antioxidant capacity based on the ability to reduce ferric ions of sample with increase in absorbance of sample and standard

Table 2: Superoxide scavenging assay

S.No

Concentration

(μg/ml)

Inhibition (%)

IC50 (μg/ml)

Rutin

Sample

Rutin

Sample

95±0.026

61±0.377

18

59

30±0.226

05±0.038

77±0.234

06±0.029

42±0.261

28±0.290

07±0.062

73±0.186

IC50: Inhibitory concentration 50%

Table 3: FRAP assay

S.No

Concentration

(μg/ml)

Absorbance

Rutin

Sample

134±0.007

094±0.005

162±0.023

127±0.009

189±0.006

149±0.008

216±0.005

181±0.011

244±0.020

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About this article

Title

EVALUATION OF IN VITRO- ANTI-OXIDANT POTENTIAL OF AQUEOUS ROOT EXTRACT OF CLERODENDRUM SERRATUM L.

DOI

10.22159/ajpcr.2017.v10i4.16930

Date

01-04-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 402-404

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

98 Views | 96 Downloads

Authors & Affiliations

Raj Kumar Tiwari
sunder deep pharmacy college
India

Udayabanu Malairaman
Asst. Prof. JUIT, Wakhnaghat, HP
India

Silpi Chanda
Assoc. Prof. NIET, Pharmacy Institute, Greater Noida
India


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