EVALUATION OF IN VITRO- ANTI-OXIDANT POTENTIAL OF AQUEOUS ROOT EXTRACT OF CLERODENDRUM SERRATUM L.

  • Raj Kumar Tiwari sunder deep pharmacy college
  • Udayabanu Malairaman Asst. Prof. JUIT, Wakhnaghat, HP
  • Silpi Chanda Assoc. Prof. NIET, Pharmacy Institute, Greater Noida

Abstract

Objective: The intent  of this report  was to investigate the effect of aqueous root extract of Clerodendrum serratum L. for antioxidant activity using divergent models viz. DPPH scavenging assay, Superoxide scavenging assay and Ferric Reducing Antioxidant Power (FRAP) assay.

Materials and Methods: The root of C. serratum was extracted using water. The yield of aqueous extract was 10%w/w. The outcome was examined statistically by the regression method.

Results and discussions: The IC50 values are 85.43 µg/ml and 107.59 µg/ml for DPPH radical scavenging and Superoxide scavenging assay respectively whereas  FRAP showed significant reducing power activity with increased concentration of sample. The pilot study showed, a significant correlation existed between concentrations of the extract and percentage engrossment of free radicals.

Conclusion: The antioxidant property may be corresponding to the polyphenols and flavonoids adjacent in the extract. These results clearly revealed that C. serratum might be effective against diseases analogous with free radical mediated.

 

Keywords Clerodendrum serratum, DPPH, Superoxide, FRAP, Rutin, Antioxidant

References

1. Mohammad Y, Mohammad I. Antiasthmatic herbal drugs: A review. Int J Pharm Pharm Sci 2010;2:28-9.
2. Sumanta KG, Nonigopal G, Avijit S, Anupam G. Traditional herbal remedies for various ailments within the rural communities in the district of Bankura and Purulia, West Bengal, India. Int J Pharm Pharm Sci 2013;5:195-8.
3. Aravind G, Bhowmik D, Duraivel S, Harish G. Traditional and medicinal uses of Carica papaya. J Med Plants Stud 2013;1(1):7-15.
4. Ghosh T, Maity KT, Sengupta P, Dash KD, Bose A. Antidiabetic and in-vivo antioxidant activity of ethanolic extract of Bacopa monnieri L. Aerial parts: A possible mechanism of action. Iran J Pharm Res 2008;7(1):61-8.
5. Han X, Shen T, Lou H. Dietary polyphenols and their biological
Fig. 3: Superoxide scavenging assay whereas % superoxide scavenging inhibition is plotted against concentrations of rutin
Fig. 4: Superoxide scavenging assay whereas % superoxide scavenging inhibition is plotted against concentrations of aqueous root extract
Fig. 5: Ferric reducing antioxidant power assay showing antioxidant capacity based on the ability to reduce ferric ions of sample with increase in absorbance of sample and standard
Table 2: Superoxide scavenging assay
S.No
Concentration
(μg/ml)
Inhibition (%)
IC50 (μg/ml)
Rutin
Sample
Rutin
Sample
1
20
51.95±0.026
25.61±0.377
11.18
107.59
2
40
57.30±0.226
34.05±0.038
3
60
66.77±0.234
38.06±0.029
4
80
72.42±0.261
42.28±0.290
5
100
74.07±0.062
47.73±0.186
IC50: Inhibitory concentration 50%
Table 3: FRAP assay
S.No
Concentration
(μg/ml)
Absorbance
Rutin
Sample
1
20
0.134±0.007
0.094±0.005
2
40
0.162±0.023
0.127±0.009
3
60
0.189±0.006
0.149±0.008
4
80
0.216±0.005
0.181±0.011
5
100
0.244±0.020
0.197±0.013significance. Int J Mol Sci 2007;8(9):950-88.
6. Brieger K, Schiavone S, Miller FJ, Krause KH. Reactive oxygen species: From health to disease. Swiss Med Wkly 2012;142:13659.
7. Frei B. Cardiovascular disease and nutrient antioxidants: Role of low-density lipoprotein oxidation. Crit Rev Food Sci Nutr 1995;35(1-2):83-98.
8. Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: Current status and future prospects. J Assoc Physicians India 2004;52:794-804.
9. Suzuki T, Wakai K, Matsuo K, Hirose K, Ito H, Kuriki K, et al. Effect of dietary antioxidants and risk of oral, pharyngeal and laryngeal squamous cell carcinoma according to smoking and drinking habits. Cancer Sci 2006;97:760-7.
10. Ismail A Jr, Tan S. Antioxidant activity of selected commercial seaweeds. Malays J Nutr 2002;8(2):167-77.
11. Manjunatha BK, Krishna V, Pillaiah T. Flora of Davanagere District, Karnataka, India. New Delhi: Regency Pulications; 2004.
12. Keshavamurthy KR. Medicinal Plants of Karnataka. Bangalore: Karnataka Forest Department; 1994.
13. Ramya P, Lakshmidevi N. Studies on anti-oxidant activity of Tinospora cardifolia (Miers). J Am Sci 2010;6(10):736-43.
14. Neha P, Dushyant B. Antioxidant activity of ethanolic extract of Annona squamosa Linn bark. Int J Res Pharm Biomed Sci 2011;2:1692-7.
15. Veerapur VP, Prabhakar KR, Parihar VK, Kandadi MR, Ramakrishana S, Mishra B, et al. Ficus racemosa stem bark extract: A potent antioxidant and a probable natural radioprotector. Evid Based Complement Alternat Med 2009;6(3):317-24.
16. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem 1996;239(1):70-6.
17. Nishaa S, Vishnupriya M, Sasikumar JM, Christabel HP, Gopalakrishnan VK. Antioxidant activity of ethanolic extract of Maranta arundinacea. L tuberous rhizomes. Asian J Pharm Clin Res 2012;5(4):85-8.
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Tiwari, R. K., U. Malairaman, and S. Chanda. “EVALUATION OF IN VITRO- ANTI-OXIDANT POTENTIAL OF AQUEOUS ROOT EXTRACT OF CLERODENDRUM SERRATUM L.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 4, Apr. 2017, pp. 402-4, doi:10.22159/ajpcr.2017.v10i4.16930.
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