SURVEILLANCE OF MICROBIAL FLORA FOR INFERTILITY COUPLES IN AN INDIAN TERTIARY CARE TEACHING HOSPITAL


Mahesh Chandra Sahu, Sujata Priyadarsini Mishra, Ratna Panda, Tapan Patnaik

Abstract


Objective: Infertility has been known to cause serious social and emotional problems in India. Microorganisms may be a primary cause for this
infertility. This study was carried out to know the prevalence of microorganisms in the infertility couples of a tertiary caring teaching hospital.
Methods: One thousands of couples were screened for the infertility test presented at the outpatient Department of Obstetrics and Gynaecology.
The suspected infertility couples were tested for microbial contamination in their reproductive sample. Simultaneously, the demographic data of all
patients were documented, and all the data were analyzed with SPSS 20 software.

Result: From one thousand couples, 288 couples were found infertility symptoms, whereas 67.71% were primary infertility problems and rest were
secondary. It is due to male factor (27.08%), female factor (50%), and both (4.86%) but 18.05 were unexplained. From vaginal swabs, 186 and semen
145 microbial colony were grown. However, Chi-square (χ
) showed that there is no significant difference between the growth of microbial colony from semen or vaginal swap as p=0.06. Eight varieties of bacteria (387) and Candida albicans (37) were documented. 2

Conclusion: With respect to older ages of the infertile couples, it is more chances to infections. Antibiotic should be prescribed to primary infertility
couples and infections can be avoided the cross transmission during intercourse.

Keywords: Primary infertility, Candida, Semen, Antibiotic, Vaginal swab.


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References


Agboola A. Infertility and subinfertility. In: Akin A, editor. Textbook of Obstetrics and Gynaecology. Vol. 1. Ibadan: Heinman Educational Books; 2004. p. 174-6.

Collier J, Longmore M, Scally P. Infertility: Causes and test. In: Collier J, Longmore M, Scally P, editors. Oxford Handbook of Clinical Specialties. 6th ed. Oxford: Oxford University Press; 2003. p. 58-9.

Okonofua F, Menakaya U, Onemu SO, Omo-Aghoja LO, Bergstrom S. A case-control study of risk factors for male infertility in Nigeria. Asian J Androl 2005;7(4):351-61.

Chukudebelu WO, Esege N, Megafu U. Etiological factors in infertility in Enugu, Nigeria. Infertility 1979;2(2):193-200.

Esimai OA, Orji EO, Lasisi AR. Male contribution to infertility in Ile-Ife, Nigeria. Niger J Med 2002;11(2):70-2.

Ojengbede OA, Omonria WE, Ladipo OA. Screening for obstruction of the vas deferens in Nigerian men with azoospermia using the alpha-glucosidase reaction in semen. Afr J Med Med Sci 1992;21(2):79-81.

Adejuwon CA, Ilesanmi AO, Ode EO. Hyperprolactinaemia as a cause of male infertility in Ibadan. West Afr J Med 1999;18:17-9.

Onemu SO, Ibeh N. Studies on the significance of positive bacterial semen cultures in male fertility in Nigeria. Int Fertil Women Med 2001;46:210-4.

Olooto WE. Infertility in male; Risk factors, causes and management-A review. J Microbiol Biotechnol Res 2012;2(4):641-5.

Ohri M, Prabha V. Isolation of sperm agglutinating factor from Staphylococcus aureus isolated from a woman with unexplained infertility. Fertil Steril 2005; 84, 1539-41.

World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. Cambridge: Cambridge University Press; 1999.

Ochsendorf FR, Özdemir K, Rabenau H, Fenner T, Oremek R, Milbradt R, et al. Chlamydia trachomatis and male infertility: Chlamydia-IgA antibodies in seminal plasma are C. Trachomatis specific and associated with an inflammatory response. J Eur Acad Dermatol Venereol 1999;12(2):143-52.

Mitchell H. Vaginal discharge--causes, diagnosis, and treatment. BMJ 2004;328(7451):1306-8.

Royal College of Obstetricians & Gynaecologists; British Association for Sexual Health and HIV. The management of women of reproductive age attending non-genitourinary medicine settings complaining of vaginal discharge. J Fam Plann Reprod Health Care 2006;32(1):33-42.

Caillouette JC, Sharp CF Jr, Zimmerman GJ, Roy S. Vaginal pH as a marker for bacterial pathogens and menopausal status. Am J Obstet Gynecol 1997;176(6):1270-5.

Radcliffe K, Jusuf I, Cowan FM, Fitzgerald M, Wilson J. UK national guidelines on sexually transmitted infections and closely related conditions. Sex Transm Infect 1999;75:S1.

Blackwell AL, Thomas PD, Wareham K, Emery SJ. Health gains from screening for infection of the lower genital tract in women attending for termination of pregnancy. Lancet 1993;342(8865):206-10.

Dubey D, Rath S, Sahu MC, Rout S, Debata NK, Padhy RN. A report on infection dynamics of inducible clindamycin resistance of Staphylococcus aureus isolated from a teaching hospital in India. Asian Pac J Trop Biomed 2013;3(2):148-53.

Rath S, Dubey V, Sahu MC, Debata NK, Padhy RN. Surveillance of multidrug resistant Escherichia coli in community and a hospital from Odisha. Asian Pac J Trop Dis 2014;4(2):140-9.20. Dash A, Sahu K, Senapati JN, Debata NK, Sahoo J, Raulo BC, et al. Surveillance of antibiotic sensitivity and resistance pattern of bacteria isolated from orthopaedic wound discharge. Int J Pharm Sci Rev Res 2016;36(1):208-11.

Sahu MC, Patnaik R, Padhy RN. In vitro combination-efficacy of ceftriaxone and leaf extract of Combretum albidum G. Don against multidrug resistant Pseudomonas aeruginosa and host-toxicity testing with human lymphocytes. J Acute Med 2014;4(1):26-37.

Sahu MC, Rath S, Dubey D, Debata NK, Padhy RN. Multidrug resistance of Pseudomonas aeruginosa as known from surveillance of nosocomial and community infections in an Indian teaching hospital. J Public Health 2012;20(4):413-23.

Sahu MC, Dubey D, Rath S, Panda T, Padhy RN. Monograph: In vitro efficacy of 30 ethnomedicinal plants used by Indian aborigines against 6 multidrug resistant gram-positive pathogenic bacteria Asian Pac J Trop Dis 2015;5(2):136-50.

Sahu MC, Padhy RN. Bayesian evaluation of two conventional diagnostic methods for pathogenic fungal infections. J Acute Med 2014;4(3):109-19.

Sahu MC, Debata NK, Padhy RN. In vitro efficacy of Butea monosperma Lam. Against 12 clinically isolated multidrug resistant bacteria. Asian Pac J Trop Dis 2013;3(3):217-26.

Uh Y, Choi SJ, Jang IH, Lee KS, Cho HM, Kwon O, et al. Colonization rate, serotypes, and distributions of macrolide-lincosamide-streptogramin B resistant types of group B streptococci in pregnant women. Korean J Clin Microbiol 2009;12(4):174-9.

Bayraktar MR, Ozerol IH, Gucluer N, Celik O. Prevalence and antibiotic susceptibility of Mycoplasma hominis and Ureaplasma urealyticum in pregnant women. Int J Infect Dis 2010;14(2):e90-5.

Donati L, Di Vico A, Nucci M, Quagliozzi L, Spagnuolo T, Labianca A, et al. Vaginal microbial flora and outcome of pregnancy. Arch Gynecol Obstet 2010;281(4):589-600.

Divyashanthi CM, Adithiyakumar S, Bharathi N. Study of prevalence and antimicrobial susceptibility pattern of bacterial isolates in a tertiary care hospital. Int J Pharm Pharm Sci 2014;7(1):185-90.

Jyothi P. Antibiotic sensitivity pattern of Citrobacter spp. isolated from patients with urinary tract infections in tertiary care hospital in South India. Int J Pharm Pharm Sci 2014;7(1):252-4.

Hay PE, Morgan DJ, Ison CA, Bhide SA, Romney M, McKenzie P, et al. A longitudinal study of bacterial vaginosis during pregnancy. Br J Obstet Gynaecol 1994;101(12):1048-53.

Lee SE, Romero R, Kim EC, Yoon BH. A high Nugent score but not a positive culture for genital mycoplasmas is a risk factor for spontaneous preterm birth. J Matern Fetal Neonatal Med 2009;22(3):212-7.

Choi SJ, Park SD, Jang IH, Uh Y, Lee A. The prevalence of vaginal microorganisms in pregnant women with preterm labor and preterm birth. Ann Lab Med 2012;32:194-200.




About this article

Title

SURVEILLANCE OF MICROBIAL FLORA FOR INFERTILITY COUPLES IN AN INDIAN TERTIARY CARE TEACHING HOSPITAL

DOI

10.22159/ajpcr.2017.v10i4.16932

Date

01-04-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 405-408

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

73 Views | 65 Downloads

Authors & Affiliations

Mahesh Chandra Sahu
Directorate of Medical Research, Institute of Medical Science(IMS) and SUM Hospital, Siksha O Anusandhan University, K8, Kalinga Nagar, Bhubaneswar-03, Odisha, India
India

Sujata Priyadarsini Mishra

Ratna Panda

Tapan Patnaik


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