SYNTHESIS, IN SILICO PHYSICOCHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES OF SOME PYRAZOLINE DERIVATIVES
Objectives: Nitrogen containing heterocyclic compounds plays an important role in medicinal chemistry. Among them, five-membered ring pyrazolines have found to possess many biological and pharmacological activities like anticancer, antitubercular, antimicrobial, anti-inflammatory etc. Objective is to determine the physicochemical and drug like properties of the synthesized pyrazolines by in silico methods and to screen their antidiabetic and antioxidant activities.
Methods: Chalcones were synthesized from naphthaldehydes by condensing with various substituted acetophenones in ethanol and cyclized into pyrazolines using semicarbazides/thiosemicarbazides by conventional and microwave oven synthesis. The physicochemical and drug like properties were determined by using computational tools. Antidiabetic activity was evaluated by alpha amylase inhibition assay method. Antioxidant activity studies were done by DPPH and nitric oxide method.
Results: Pyrazolines were synthesized from chalcones. Microwave irradiated synthesis of chalcone was carried out to get higher yield with less reaction time period as compared to conventional method. The synthesized pyrazolines produces yield around 68% (conventional) and 85% (microwave). In silico studies showed considerable values satisfying all the parameters of physicochemical and Lipinski's rule of five properties.Â Among the compounds tested for antidiabetic and antioxidant studies, some showed promising activity.
Conclusion: Physiochemical and drug like properties revealed that these compounds have good bioavailability and druglikeness properties. So these compounds are found to be interesting lead molecules for further synthesis as antidiabetic and antioxidant agents.
Keywords: Chalcones, pyrazolines, in silico physicochemical properties, biological activities.
Table 4: Antioxidant activity of pyrazolines
Inhibition level, % at 200 Î¼M
DPPH: 2,2â€‘diphenylâ€‘1â€‘picrylhydrazyl, NO: Nitric oxide2014;7(4):237-9.
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