ANTICHOLINESTERASE ACTIVITY OF OCTA PEPTIDES RELATED TO HUMAN HISTATIN 8: IN-SILICO DRUG DESIGN AND IN-VITRO


Pandurangan Perumal, Mani Vasudevan, Sridevi Chigurupati, Manikandan Selvaraj

Abstract


Objective: To evaluate the octapeptides related to human histatin 8 by in-silico and in-vitro studies.

Method: Schrodinger, LLC and Ellman’s method.

Results: The compound HH1 and HH2 was found to be potent docking score of −9.494 and −7.401 against acetylcholinesterase (AChE) enzyme. The IC50 value of HH1 and HH2 was found to be 0.39±0.28 and 0.78±0.15 μg/mL. However, these compounds are shown to be highly effective as compared with the control AChE inhibitor donepezil (0.065±0.0050 μg/mL).

Conclusion: In-silico docking study was conducted for the designed octapeptides related to human histatin 8 against AChE enzyme shows significance binding affinity toward HH1 and HH2 peptides and the AChE inhibitory activity of octapeptides shown to be a highly potent inhibitor as compared with control donepezil.


Keywords


Alzheimer’s disease, Octapeptides, In-silico, In-vitro, Acetyl cholinesterase.

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About this article

Title

ANTICHOLINESTERASE ACTIVITY OF OCTA PEPTIDES RELATED TO HUMAN HISTATIN 8: IN-SILICO DRUG DESIGN AND IN-VITRO

Keywords

Alzheimer’s disease, Octapeptides, In-silico, In-vitro, Acetyl cholinesterase.

DOI

10.22159/ajpcr.2017.v10i6.17697

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 115-117

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

138 Views | 103 Downloads

Authors & Affiliations

Pandurangan Perumal
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology University, 08100 Bedong, Kedah Darul Aman, Malaysia.
Malaysia

Mani Vasudevan
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Al Qassim University, Qassim, Al qassim, Saudi Arabia.
Saudi Arabia

Sridevi Chigurupati
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Asian Institute of Medicine, Science and Technology University, 08100 Bedong, Kedah Darul Aman, Malaysia.
Malaysia

Manikandan Selvaraj
Department of Bio-informatics Centre, Integrative Pharmacogenomics Institute, Universiti Teknologi MARA, 42300 Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia.
Malaysia


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