SCREENING OF BIOPROTECTIVE PROPERTIES OF VARIOUS PLANT EXTRACTS AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING OF ADENANTHERA PAVONINA STEM EXTRACT


Shubha Bhadran, Sangeetha Annie George, Sudhakar Malla, Harini Bp

Abstract


Objective: The search for various phytotherapeutic compounds is on rise due to a complex multifactorial phenomenon called drug resistance. The present study investigates the cytotoxic, antioxidant, and antiproliferative potential of methanolic extracts of Clitorea ternatea, Averrhoa bilimbi, Phyllanthus acidus, Tecoma stans, Curcuma aromatica, Anethum graveolens, Adhatoda vasica, Markhamia lutea, Spathodea companulata, and Adenanthera pavonina.

Methods: The plant parts were extracted with methanol and screened for 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging abilities. The cytotoxic activity of the extracts was investigated on HeLa and HCT116 cells through 3-(4,5-dimethylthiazol2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and cell cycle was analyzed by flow cytometry to determine the antiproliferative activity of the extracts. The stem extract of A. pavonina was further subjected to gas chromatography-mass spectrometry (GC-MS) analysis for purification of the compounds of interest. A two-way ANOVA was done to estimate the effect of the extract between samples remembered at p<0.05 level.

Results: Among all the studied samples, the extract of A. pavonina (stem) showed significant scavenging activity of 70.23% and 76.32% of scavenging compared to 74.58 % and 81.13% of that of reference standard in ABTS and DPPH assay, respectively. GC-MS analysis of the extract revealed the presence of 17 phytocompounds. MTT assay revealed that this extract (SB19) had promising cytotoxic activity against the two cancer cell lines, HCT116 and HeLa with inhibitory concentration 50% IC50 values of 25.86±0.21 μg/ml and 39.89±0.11 μg/ml, respectively. The extract treatment caused significant arrest in G2M phase of cell cycle.

Conclusion: A. pavonina (stem) extract displayed significant antioxidant and antiproliferative activity and can be considered as a potential candidate drug for anticancer studies.


Keywords


Antioxidants, 3-(4,5-dimethylthiazol2yl)-2,5-diphenyl tetrazolium bromide assay, Antiproliferative, Gas chromatography-mass spectrometry, Adenanthera pavonina.

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References


Okigbo RN, Anuagasi CL, Amadi JE. Advances in selected medicinal and aromatic plants indigenous to Africa. J Med Plants Res 2009;3(2):86-95.

Okwu DE. Phytochemicals and vitamin content of indigenous spices of South Eastern Nigeria. J Sustain Agric Environ 2004;6:30-4.

Moreira P, Smith MA, Zhu X, Honda K, Lee HG, Aliev G, et al. Since oxidative damage is a key phenomenon in Alzheimer’s disease, treatment with antioxidants seems to be a promising approach for slowing disease progression. Oxidative damage and Alzheimer’s disease: Are antioxidant therapies useful? Drug News Perspect 2005;18:13-9.

Paz-Elizur T, Sevilya Z, Leitner-Dagan Y, Elinger D, Roisman LC, Livneh Z. DNA repair of oxidative DNA damage in human carcinogenesis: Potential application for cancer risk assessment and prevention. Cancer Lett 2008;266(1):60-72.

Naito Y, Uchiyama K, Yoshikawa T. Oxidative stress involvement in diabetic nephropathy and its prevention by astaxanthin. Oxid Stress Dis 2006;21:235-42.

Jain SK. Superoxide dismutase overexpression and cellular oxidative damage in diabetes. A commentary on “Overexpression of mitochondrial superoxide dismutase in mice protects the retina from diabetes-induced oxidative stress”. Free Radic Biol Med 2006;41(8):1187-90.

Heinecke JW. Mechanisms of oxidative damage of low density lipoprotein in human atherosclerosis. Curr Opin Lipidol 1997;8(5):268-74.

Halliwell B. How to characterize a biological antioxidant. Free Radic Res Commun 1990;9(1):1-32.

Halliwell B. Antioxidants: The basics - What they are and how to evaluate them. Adv Pharmacol 1997;38:3-20.

Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006;160(1):1-40.

Wattenberg LW. Chemoprevention of carcinogenesis by minor dietary constituents: Symposium introduction. Pharm Biol 1998;36:6-7.

Yang CS, Chung JY, Yang G, Chhabra SK, Lee MJ. Tea and tea polyphenols in cancer prevention. J Nutr 2000;130 2S Suppl:472S-8.

Greenberg ER, Baron JA, Tosteson TD, Freeman DH Jr, Beck GJ, Bond JH, et al. A clinical trial of antioxidant vitamins to preventcolorectal adenoma. Polyp Prevention Study Group. N Engl J Med 1994;331(3):141-7.

Weng CJ, Yen GC. Chemopreventive effects of dietary phytochemicals against cancer invasion and metastasis: Phenolic acids, monophenol, polyphenol, and their derivatives. Cancer Treat Rev 2012;38(1):76-87.

Yang CS, Li G, Yang Z, Guan F, Chen A, Ju J. Cancer prevention by tocopherols and tea polyphenols. Cancer Lett 2013;334(1):79-85.

Auddy B, Ferreira M, Blasina F, Lafon L, Arredondo F, Dajas F, et al. Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases. J Ethnopharmacol 2003;84(2-3):131-8.

Gaitry CP, Binda DS, Farhin I, Sujata SD. Antimicrobial and antioxidant activities of methanol extract roots of Glycyrrhiza glabra and HPLC analysis. Int J Pharm Pharm Sci 2013;5:975-1491.

Zahin M, Aqil F, Ahmad I. The in vitro antioxidant activity and total phenolic content of four Indian medicinal plants. Int J Pharm Pharm Sci 2009;1 Suppl 1:88-95.

Niki E. Assessment of antioxidant capacity in vitro and in vivo. Free Radic Biol Med 2010;49(4):503-15.

Johnson IT. Phytochemicals and cancer. Proc Nutr Soc 2007;66(2):207-15.

Langseth L. Oxidants, Antioxidants, and Disease Prevention. Washington, DC, USA: International Life Sciences Institute Press; 1995.

Johnson IT. New approaches to the role of diet in the prevention of cancers of the alimentary tract. Mutat Res 2004;551(1-2):9-28.

Silva IK, Soysa P. Evaluation of phytochemical composition and antioxidant capacity of a decoction containing Adenanthera pavonina L. and Thespesia populnea L. Pharmacogn Mag 2011;7(27):193-9.

Ramli S, Bunrathep S, Tansaringkarn T, Ruangrungsi N. Screening for free radical scavenging activity from ethanolic extract of mimosaceous plants endemic to Thailand. J Health Res 2008;22:55-9.

Rahman M, Habib R, Hasan SM, Sayeed MA, Rana S. Antibacterial, cytotoxic and antioxidant potential of methanolic extract of Phyllanthus acidus L. Int J Drug Dev Res 2011;3(2):154-61.

Govindappa M, Sadananda TS, Channabasava R, Jeevitha MK, Pooja KS, Raghavendra VB. Antimicrobial, antioxidant activity and phytochemical screening of Tecoma stans (L.) Juss. Ex Kunth. J Phytol 2011;3(3):68-76.

Heim SC, Guarnier FA, Ferreira DT, Braz-Filho R, Cecchini R, Cecchini AL. Antioxidant activity of Spathodea campanulata (Bignoneaceae) extracts. Rev Bras Plantas Med Botucatu 2012;14(2):287-92.

Aladesanmi AJ, Iwalewa EO, Adebajo AC, Akinkunmi EO, Taiwo BJ, Olorunmola FO, et al. Antimicrobial and antioxidant activities of some Nigerian medicinal plants. Afr J Tradit Complement Altern Med 2006;4(2):173-84.

Kumar A, Garg VK, Kumar R, Singh L, Chauhan S. Pharmacognostic study and establishment of quality parameters of leaves of Adhatoda vasica. Linn. J Med Plants Stud 2013;1(3):35-40.

Kumar KA, Gousia SK, Anupama M, Latha JN. A review on phytochemical constituents and biological assays of Averrhoa bilimbi. Int J Pharm Pharm Sci Res 2013;3(4):136-9.

Gopinath G, Sujesh M, Babu TD. Evaluation of cytotoxic and anti-tumor activity of Phyllanthus acidus (L.) Skeels leaf extracts. Int J Nov Res Life Sci 2015;2(2):19-26.

Kulkarni AA. Ray of hope for cancer patients. In: Proceedings of the International Seminar on Holistic Management of Cancer, Ayurvedic Education: Series No. 67; 1998. p. 5-11.

Hassan R, El-Kadi S, Mostafa S. Effect of some organic acids on some fungal growth and their toxins production. Int J Adv Biol 2015;2:1-11.

Küçük HB, Yusufoglu A, Mataraci E, Dösler S. Synthesis and biological activity of new 1,3-dioxolanes as potential antibacterial and antifungal compounds. Molecules 2011;16(8):6806-15.

Delcourt A, Mathieu G, Baji H, Kimny T, Flammang M, Compagnon PL. New polyazole derivatives from 2-(2,4-dichlorophenyl)-1,3-dioxolane. Antifungal activity. Structure-activity relationships. Mycopathologia 1997;137(1):27-32.

Coleman MD, Zilz TR, Griffiths HR, Woehrling EK. A comparison of the apoptotic and cytotoxic effects of hexanedione derivatives on human non-neuronal lines and the neuroblastoma line SH-SY5Y. Basic Clin Pharm Toxicol 2007;102:25-9.

Zilz TR, Griffiths HR, Coleman MD. Apoptotic and necrotic effects of hexanedione derivatives on the human neuroblastoma line SK-N-SH. Toxicology 2007;231(2-3):210-4.

Baji H, Kimny T, Gasquez F, Flammang M, Compagnon PL, Delcourt A, et al. Synthesis, antifungal activity and structure-activity relationships of 2-(alkyl or aryl)-2-(alkyl or polyazol-1-ylmethyl)-4-(polyazol-1-ylmethyl)-1,3-dioxolanes. Eur J Med Chem 1997;32:637-50.

Crawley GC, Briggs MT. Chiral dioxolane inhibitors of leukotriene biosynthesis: Structure-activity relationships and syntheses using asymmetric dihydroxylation. J Med Chem 1995;38(20):3951-6.

Genta MT, Villa C, Mariani E, Loupy A, Petit A, Rizzetto R, et al. Microwave-assisted preparation of cyclic ketals from a cineole ketone as potential cosmetic ingredients: Solvent-free synthesis, odour evaluation, in vitro cytotoxicity and antimicrobial assays. Int J Pharm 2002;231(1):11-20.

Shirai R, Takayama H, Nishikawa A, Koiso Y, Hashimoto Y. Asymmetric synthesis of antimitotic combretadioxolane with potent antitumor activity against multi-drug resistant cells. Bioorg Med Chem Lett 1998;8(15):1997-2000.




About this article

Title

SCREENING OF BIOPROTECTIVE PROPERTIES OF VARIOUS PLANT EXTRACTS AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING OF ADENANTHERA PAVONINA STEM EXTRACT

Keywords

Antioxidants, 3-(4,5-dimethylthiazol2yl)-2,5-diphenyl tetrazolium bromide assay, Antiproliferative, Gas chromatography-mass spectrometry, Adenanthera pavonina.

DOI

10.22159/ajpcr.2017.v10i7.18141

Date

01-07-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 7 July 2017 Page: 188-194

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

83 Views | 83 Downloads

Authors & Affiliations

Shubha Bhadran
Department of Genetics, Indian Academy Centre for Research & Post Graduate Studies, Bengaluru, Karnataka, India.
India

Sangeetha Annie George
Department of Zoology, Indian Academy Centre for Research & Post Graduate Studies, Bengaluru, Karnataka, India.
India

Sudhakar Malla
Department of Biotechnology, Indian Academy Centre for Research & Post Graduate Studies, Bengaluru, Karnataka, India.
India

Harini Bp
Department of Zoology, Bangalore University, Bengaluru, Karnataka, India.
India


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