EVALUATION OF ANTIMICROBIAL, CYTOTOXICITY, AND DYEING PROPERTIES OF PRODIGIOSIN PRODUCED BY SERRATIA MARCESCENS STRAIN JAR8


Ritika Chauhan, Aniket Choudhuri, Jayanthi Abraham

Abstract


 

 Objective: Prodigiosin is a red tripyrrole pigment which is synthesized as a secondary metabolite by biotypes A1, A2, and A6 of Serretia marcescens. This study was aimed to optimize production parameters for prodigiosin pigment produced by S. marcescens strain JAR8 and to determine its antimicrobial, cytotoxicity, and dyeing properties.

Methods: The effect of various media components, process parameters (temperature, pH, incubation period), and other supplements was investigated, and the maximum production of prodigiosin was found to be at temperature 28°C, pH 7.0, incubation period of 5 day with peptone in the media. Antibacterial, cytotoxicity, and dyeing properties of prodigiosin were studied in depth.

Results: The antimicrobial study of prodigiosin revealed that it is a potent inhibitor of Gram-positive bacteria as well as Gram-negative bacteria. The prodigiosin exhibited commendable dose-dependent cytotoxicity effect against human bone cancer cells with the IC50 of 108 μg/ml. Prodigiosin produced by strain JAR8 resisted the action of acid, alkali and detergent when applied to textile cloth.

Conclusion: The deep red pigment prodigiosin produced by strain JAR8 is economically effective and can be categorized as microbial natural product for further pharmaceutical applications.


Keywords


Biopigments, Clinical pathogens, Cytotoxicity, Natural products, Osteosarcoma cells, Prodigiosin.

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About this article

Title

EVALUATION OF ANTIMICROBIAL, CYTOTOXICITY, AND DYEING PROPERTIES OF PRODIGIOSIN PRODUCED BY SERRATIA MARCESCENS STRAIN JAR8

Keywords

Biopigments, Clinical pathogens, Cytotoxicity, Natural products, Osteosarcoma cells, Prodigiosin.

DOI

10.22159/ajpcr.2017.v10i8.18173

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 8 August 2017 Page: 279-283

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

217 Views | 120 Downloads

Authors & Affiliations

Ritika Chauhan
Department of Biotechnology , The Oxford College of Science, HSR Layout, Bengaluru, Karnataka, India
India

Aniket Choudhuri
Department of Food Science and Agriculture Chemistry, Mcgill MacDonald Campus, Sainte Anne de Bellevue, Montreal, Canada.
India

Jayanthi Abraham
Department of Agricultural Chemistry and Environmental Science, Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India.
India


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