BIOSYNTHESIS OF TITANIUM DIOXIDE NANOPARTICLES USING VIGNA RADIATA

Authors

  • Ankita Chatterjee
  • Nishanthini D Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India.
  • Sandhiya N Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India.
  • Jayanthi Abraham Dr. Jayanthi Abraham, M.Phil., Ph.D., Professor, Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India. Email: jayanthi.abraham@gmail.com Mobile: +91- 9843580709

Abstract

ABSTRACT
Objectives: Nanotechnology holds an important area in recent research due of its immense use in the different field of sciences. The small size, large
surface area, orientation, and physical properties make them appropriate to be used in medical sciences, physics, and chemistry. Physical and chemical
processes of synthesizing nanoparticles are continued for ages. The biological approaches to preparing nanoparticles have drawn the attention of
researchers due to eco-friendly nature, low cost, and easier steps for synthesis. To synthesize titanium nanoparticles, Vigna radiata legumes were
powdered and used, and the effectiveness of biologically synthesized titanium nanoparticles against clinical pathogens and anticancer activity were
checked.
Methods: Green synthesis of titanium dioxide nanoparticles is carried out in simple steps. The extract of V. radiata legumes was used for the biological
synthesis of the titanium dioxide nanoparticles which was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy.
Antibacterial activity of the titanium dioxide nanoparticles was checked against nine clinical pathogens. 2,2-diphenyl-1-picryl-hydrazyl-hydrate
assay was performed to determine the antioxidant activity of the nanoparticles. Cytotoxic activity against osteosarcoma cell lines was performed by
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Results: Oval shaped titanium nanoparticles were biologically synthesized. The particles showed effectiveness against both Gram-positive and Gramnegative
bacteria.
The particles
exhibited
antioxidant
nature.
Cytotoxicity
assay
revealed
the nanoparticles were
capable of
inhibiting
proliferation
of
osteosarcoma
cell lines.
Conclusions: V. radiata has been confirmed to be a good source for biosynthesis of titanium nanoparticles. The cytotoxicity effect of the nanoparticles
hints that it can be further used for treatment and medicine purposes.
Keywords: Green synthesis, Titanium dioxide, Vigna radiata, Fourier transform infrared spectroscopy, Scanning electron microscopy, 2,2-diphenyl-1picryl-hydrazyl-hydrate,
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide.

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Published

2016-07-01

How to Cite

Chatterjee, A., N. D, S. N, and J. Abraham. “BIOSYNTHESIS OF TITANIUM DIOXIDE NANOPARTICLES USING VIGNA RADIATA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 4, July 2016, pp. 85-88, https://innovareacademics.in/journals/index.php/ajpcr/article/view/11301.

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