• Ankita Chatterjee
  • Nishanthini D Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India.
  • Sandhiya N Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India.
  • Jayanthi Abraham Dr. Jayanthi Abraham, M.Phil., Ph.D., Professor, Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India. Email: Mobile: +91- 9843580709


Objectives: Nanotechnology holds an important area in recent research due of its immense use in the different field of sciences. The small size, large
surface area, orientation, and physical properties make them appropriate to be used in medical sciences, physics, and chemistry. Physical and chemical
processes of synthesizing nanoparticles are continued for ages. The biological approaches to preparing nanoparticles have drawn the attention of
researchers due to eco-friendly nature, low cost, and easier steps for synthesis. To synthesize titanium nanoparticles, Vigna radiata legumes were
powdered and used, and the effectiveness of biologically synthesized titanium nanoparticles against clinical pathogens and anticancer activity were
Methods: Green synthesis of titanium dioxide nanoparticles is carried out in simple steps. The extract of V. radiata legumes was used for the biological
synthesis of the titanium dioxide nanoparticles which was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy.
Antibacterial activity of the titanium dioxide nanoparticles was checked against nine clinical pathogens. 2,2-diphenyl-1-picryl-hydrazyl-hydrate
assay was performed to determine the antioxidant activity of the nanoparticles. Cytotoxic activity against osteosarcoma cell lines was performed by
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Results: Oval shaped titanium nanoparticles were biologically synthesized. The particles showed effectiveness against both Gram-positive and Gramnegative
The particles
the nanoparticles were
capable of
cell lines.
Conclusions: V. radiata has been confirmed to be a good source for biosynthesis of titanium nanoparticles. The cytotoxicity effect of the nanoparticles
hints that it can be further used for treatment and medicine purposes.
Keywords: Green synthesis, Titanium dioxide, Vigna radiata, Fourier transform infrared spectroscopy, Scanning electron microscopy, 2,2-diphenyl-1picryl-hydrazyl-hydrate,



Sastry M, Ahmad A, Khan MI, Kumar R. Biosynthesis of metal

nanoparticles using fungi and actinomycete. Curr Sci 2003;85(2):162-70.

Singh N, Saha P, Rajkumar K, Abraham J. Biosynthesis of silver and

selenium nanoparticles by Bacillus sp. JAPSK2 and evaluation of

antimicrobial activity. Pharm Lett 2014;6(1):175-81.

Singh N, Chatterjee A, Chakraborty K, Chatterjee S, Abraham J.

Cytotoxic effect on MG-63 cell line and antimicrobial and antioxidant

properties of silver nanoparticles synthesized with seed extracts of

Capsicum sp. Rec Nat Prod 2016;10(1):47-57.

MubarakAli D, Thajuddin N, Jeganathan K, Gunasekaran M. Plant

extract mediated synthesis of silver and gold nanoparticles and its

antibacterial activity against clinically isolated pathogens. Colloids

Surf B Biointerfaces 2011;85(2):360-5.

Kirthi AV, Rahuman AA, Rajakumar G, Marimuthu S, Santhoshkumar T,

Jayaseelan C, et al. Biosynthesis of titanium dioxide nanoparticles

using bacterium Bacillus subtilis. Mater Lett 2011;65(17):2745-7.

Rajakumar G, Rahuman AA, Priyamvada B, Khanna VG, Kumar DK,

Sujin PJ. Eclipta prostrata leaf aqueous extract mediated synthesis of

titanium dioxide nanoparticles. Mater Lett 2012;68:115-7.

Parashar V, Parashar R, Sharma B, Pandey AC. Parthenium leaf extract

mediated synthesis of silver nanoparticles: A novel approach towards

weed utilization. Dig J Nanomater Biosci 2009;4(1):45-50.

Nadagouda MN, Iyanna N, Lalley J, Han C, Dionysiou DD, Varma RS.

Synthesis of silver and gold nanoparticles using antioxidants from

blackberry, blueberry, pomegranate, and turmeric extracts. ACS Sustain

Chem Eng 2014;2(7):1717-23.

Ibrahem KH, Salman JA, Ali FA. Effect of titanium nanoparticles

biosynthesis by Lactobacillus crispatus on urease, hemolysin and

Asian J Pharm Clin Res, Vol 9, Issue 4, 2016, 85-88

Chatterjee et al.

biofilm forming by some bacteria causing recurrent UTI in Iraqi

women. Eur Sci J 2014;10(9):324-38.

Sundrarajan M, Gowri S. Green synthesis of titanium dioxide

nanoparticles by Nyctanthes arbor-tristis leaves extract. Chalcogenide

Lett 2011;8(8):447-51.

Santhoshkumar T, Rahuman AA, Jayaseelan C, Rajakumar G,

Marimuthu S, Kirthi AV, et al. Green synthesis of titanium dioxide

nanoparticles using Psidium guajava extract and its antibacterial and

antioxidant properties. Asian Pac J Trop Med 2014;7(14):968-76.

Rehman A, Prabhakar BT, Vijay BR, Aditya SJ, Ramesh CK.

Antioxidant potentials in sprouts vs. seeds of Vigna radiata and

Macrotyloma uniflorum. J Appl Pharm Sci 2011;1(7):99.

Prasad C, Venkateswarlu P. Soybean seeds extract based green synthesis

of silver nanoparticles. Indian J Adv Chem Sci 2014;2(3):208-11.

Chauhan R, Kumar A, Abraham J. A biological approach to the synthesis

of silver nanoparticles with Streptomyces sp JAR1 and its antimicrobial

activity. Sci Pharm 2013;81(2):607-21.

Azad B, Banerjee A. Formulation of silver nanoparticles using

methanolic extract of stem of plant Desmodium gangeticum, their

characterization and antibacterial and anti-oxidant evaluation. Pharm

Innov 2014;3(7):77-81.

Satyavani K, Gurudeeban S, Ramanathan T, Balasubramanian T.

Toxicity study of silver nanoparticles synthesized from Suaeda monoica

on Hep-2 cell line. Avicenna J Med Biotechnol 2012;4(1):35-9.

Shahab M, Tabish T, Zaman B, Tariq Z, Kamran M. Characterization

and synthesis of nanosized TiO

particles. Ann Fac Eng Hunedoara Int

J Eng 2013;11(3):313-6.

Vetrivel V, Rajendran K, Kalaiselvi V. Synthesis and characterization of

pure titanium dioxide nanoparticles by Sol-gel method. Int J ChemTech

Res 2015;7(3):1090-7.

Sharmila Devi R, Venckatesh R, Sivaraj R. Synthesis of titanium

dioxide nanoparticles by sol-gel technique. Int J Innov Res Sci Eng

Technol 2014;3(8):15206-11.

Rajakumar G, Rahuman AA, Roopan SM, Khanna VG, Elango G,

Kamaraj C, et al. Fungus-mediated biosynthesis and characterization

of TiO

nanoparticles and their activity against pathogenic bacteria.

Spectrochim Acta Mol Biomol Spectrosc 2012;91:23-9.

Kalyanasundharam S, Prakash MJ. Biosynthesis and characterization

of titanium dioxide nanoparticles using Pithecellobium dulce and

Lagenaria siceraria aqueous leaf extract and screening their free

radical scavenging and antibacterial properties. Int Lett Chem Physics

Astron 2015;50:80.

Petchiammal C, Hopper WA. Antioxidant activity of proteins from

fifteen varieties of legume seeds commonly consumed in India. Int J

Pharm Pharm Sci 2014;6(l-2):476-9.

Suker DK, Albadran RM. Cytotoxic effects of titanium dioxide

nanoparticles on rat embryo fibroblast REF-3 cell line in v



How to Cite

Chatterjee, A., N. D, S. N, and J. Abraham. “BIOSYNTHESIS OF TITANIUM DIOXIDE NANOPARTICLES USING VIGNA RADIATA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 4, July 2016, pp. 85-88,



Original Article(s)