EDEMA-REDUCING ACTIVITY OF SARGASSUM CRASSIFOLIUM Î’-GLUCAN (LAMINARAN) ON EDEMA-INDUCED RATTUS NORVEGICUS

  • Anies Chamidah Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Indonesia.
  • Asep A Prihanto Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Indonesia. http://orcid.org/0000-0003-4180-1325
  • Hardoko Hardoko Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Indonesia.

Abstract

Objectives: The aim of this study is to investigate anti-inflammatory activity of β-glucan (laminaran) of brown algae, Sargassum crassifolium which was extracted by using two different methods, β-glucan laminaran acid extract (LAE) and laminaran modified extract (LME).

Methods: β-glucan (laminaran) of brown algae, S. crassifolium have been extracted using two different methods, namely, β-glucan LAE and LME. The edema reducing activity of the extracts was investigated with carrageenan- induced leg edema male of Rattus norvegicus as a model.

Results: The swelling of edema was strongly inhibited by the treatment of LME with a percentage of 81.62% after 3rd hr. It also found that the size of edema were inhibited by all treatments with the value ranging from 25.49% to 81.68%. Smallest edema size was found on LME treatment, with a size of 0.68±0.09 mm where the control was about 0.92 mm. Furthermore, all β-glucan-treated mice showed lower edema size compared to control.

Conclusion: It was obvious that all type of laminaran had anti-inflammatory activity. LME is the best method for production of β-glucan (laminaran) with high anti-inflammatory activity. Hence, in this study, we reported a new anti-inflammatory agent derived from S. crassifolium.

Keywords: β-glucan (laminaran), Edema, Sargassum crassifolium, Carrageenan, Edema.

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Chamidah, A., A. A. Prihanto, and H. Hardoko. “EDEMA-REDUCING ACTIVITY OF SARGASSUM CRASSIFOLIUM Î’-GLUCAN (LAMINARAN) ON EDEMA-INDUCED RATTUS NORVEGICUS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 7, July 2017, pp. 311-3, doi:10.22159/ajpcr.2017.v10i7.18523.
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