HAIR GROWTH ACTIVITY AND SAFETY TEST OF ETHOSOMAL GEL ETHYL ACETATE FRACTION OF NOTHOPANAX LEAVES (NOTHOPANAX SCUTELLARIUM MERR.)

  • Mahdi Jufri Department of Pharmaceutical Science, Faculty of Pharmacy Universitas Indonesia, Depok 16424, West Java, Indonesia
  • Lili Amelia Department of Herbal Medicine, Faculty of Pharmacy Universitas Indonesia, Depok 16424, West Java, Indonesia.
  • Abdul Munim Department of Pharmacognosy- Phytochemistry, Faculty of Pharmacy Universitas Indonesia, Depok 16424, West Java, Indonesia.

Abstract

Objective: The aims of this study were to know the hair growth activity from the ethosomal gel of ethyl acetate fraction compared to the nonethosomal gel of ethyl acetate fraction, as well as its ability to penetrate into the skin and its safety from ethosomal gel testing.

Methods: The hair growth activity was measured by three parameters: Hair length, hair thickness, and hair weight.

Results: The results obtained are shown that the ethosomal gel with concentration 1% has better hair growth activity rather than the ethosomal gel with concentration 0.5% and the nonethosomal gel but it has similarity to the positive control (minoxidil gel with concentration 2%). In vitro penetration test using Franz diffusion cells shown that the ethosomal gel with concentration 1% has cumulative penetration of quercetin higher than nonethosomal gel, which value was 3.3175±0.02 μgcm−2 for the ethosomal gel with concentration 1%; and 2.7663±0.19 μgcm-2 for the nonethosomal gel with flux values for the ethosomal gel with concentration 1% and the nonethosomal gel, respectively, are 0.4147±0.01 μgcm−2h−1 and 0.3458±0.02 μgcm−2h−1.

Conclusion: As for the safety test using hen’s egg test-chorioallantoic membrane (HET- CAM) safety test) have shown that both concentrations 0.5% and 1% of the ethosomal gel can generate mild irritation for topical application.

Keywords: Nothopanax scutellarium, Ethosome, Gel, Hair growth, Franz diffusion cell, Hen’s egg test-chorioallantoic membrane

Author Biographies

Mahdi Jufri, Department of Pharmaceutical Science, Faculty of Pharmacy Universitas Indonesia, Depok 16424, West Java, Indonesia
Pharmaceutics
Lili Amelia, Department of Herbal Medicine, Faculty of Pharmacy Universitas Indonesia, Depok 16424, West Java, Indonesia.
Department of Pharmacognosy-Phytochemistry

References

1. Paus R, Peters EM, Eichmüller S, Botchkarev VA. Neural mechanisms of hair growth control. J Investig Dermatol Symp Proc 1997;2(1):61-8.
2. Dallob AL, Sadick NS, Unger W, Lipert S, Geissler LA, Nguyen HH, et al. The effect of finasteride, a 5 alpha-reductase inhibitor, on scalp skin testosterone and dihydrotestosterone concentrations in patients with male pattern baldness. J Clin Endocrinol Metab 1994;79:703-6.
3. Heyne K. Tumbuhan Berguna Indonesia Jilid III. Jakarta: Badan Litbang Kehutanan; 1987.
4. Dalimartha S. Atlas Tumbuhan Obat Indonesia Jilid I. Ungaran: Trubus Agriwidya, Hal 87; 1999.
5. Sa’diah S, Herlina N, Indriati D. Efektifitas sediaan emulsi ekstrak etanol 70% daun mangkokan (Nothopanax scutellarius (Burm. F) Merr sebagai perangsang pertumbuhan rambut. Fitofarmaka 2014;4(1):2015.
6. Upadhyay S, Upadhyay R, Vinode R, Dixit VK. Effect of ethanolic fraction of Hibiscus rosa-sinensis, L. Leaves in androgenic alopecia. Egypt Dermatol Online J 2013;9(2):5.
7. Perez-Vizcanio F, Ibarra M, Cogolludo AL, Duarte J, Zaragoza-Arnaez F, Moreno L, et al. Endothelium-independent vasodilator effects of the flavonoid quercetin and its methylated metabolites in rat conductance and resistance arteries. J Pharmacol Exp Ther 2002;302(1):66-72.
8. Wikramanayake TC, Villasante AC, Mauro LM, Perez CI, Schachner LA, Jimenez JJ. Prevention and treatment of alopecia areata with quercetin in the C3H/HeJ mouse model. Cell Stress Chaperones 2012;17(2):267-74.
9. Pratima NA, dan Tiwari S. Ethosomes: A novel tool for transdermal drug delivery. Int J Res Pharm Sci 2012;2(1):1-20.
10. Park SN, Lee MH, Kim SJ, Yu ER. Preparation of quercetin and rutin-loaded ceramide liposomes and drug-releasing effect in liposome-in-hydrogel complex system. Biochem Biophys Res Commun 2013;435(3):361-6.
11. Rifkia V. Uji Keamanan Dan Uji Aktivitas Pertumbuhan Rambut Fraksi Etil Asetat Daun Mangkokan (Nothopanax scutellarium Merr.) Dalam Sediaan Tonik Rambut. Tesis. Depok: Fakultas Farmasi Program Studi Magister Herbal Universitas Indonesia; 2014.
12. Tanaka S, Saito M, Tabata M. Bioassay of Crude Drugs for Hair Growth Promoting Activity in Mice by a New Simple Method, Faculty of Phamaceutical Sciences. Kyoto, Japan: Kyoto Universitiy; 1980.
13. Cazedey EC, Carvalho FC, Fiorentino FA, Gremiao MP, Salgado HR. Corositex®, BCOP and HET-CAM as alternative methods to animal experimentation. Braz J Pharm Sci 2009;45(4):???.
14. Thakker KD, Chern WH. Development and validation of in vitro release tests for semisolid dosage forms-case study. Dissolution Technol 2003;10(2):10-6.
15. Sebenta M, Devina V, Tahir K. Talegaonkar sushama, iqbal zeenat; Extraction of a water soluble bioactive hypoxide and its development into an ethosmoal system for deep dermal delivery. Int J Pharm Pharm Sci 2015;7:975-1491.
16. Magdy M, Amna AM, Menna MA. Formulation and characterization of ethosomes beraing vancomycin hydrochloride for transdermal delivery. Int J Pharm Pharm Sci 2014;6:975-1491.
17. Malvern. Zetasizer Nano User Manual. United Kingdom: Malvern; 2013.
18. Interagency Coordinating Committee on the Validations of Alternative Methods. ICCVAM-Recommended Test Method Protocol: Hen’s Egg Test - Chorioallantoic Membrane (HET-CAM) Test Method; 2010. Available from: http://www.iccvam.niehs.nih.gov/methods/ocutox/MildMod-TMER.htm.
19. Transethosmoes and ethosomes for enhanced transdermal delivery of ketoralac tromethamine: A comparative assesment. Int J Cur Pharma Res 2014;6:88-93.
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How to Cite
Jufri, M., L. Amelia, and A. Munim. “HAIR GROWTH ACTIVITY AND SAFETY TEST OF ETHOSOMAL GEL ETHYL ACETATE FRACTION OF NOTHOPANAX LEAVES (NOTHOPANAX SCUTELLARIUM MERR.)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 8, Aug. 2017, pp. 134-8, doi:10.22159/ajpcr.2017.v10i8.18866.
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Original Article(s)