• Maged A. El Wakeel Associate professor of Child Health, National Research Centre, Cairo, Egypt
  • Mohamed Abou-el-asrar Professor of Pediatrics, Faculty of Medicine, Ain-Shams University, Egypt
  • Ghada Mahmoud El-kassas Associate professor of Child Health, National Research Centre, Cairo, Egypt
  • Mona A Elabd Researcher of Child Health, National Research Centre, Cairo, Egypt
  • Dina Abu Zeid Researcher of Child Health, National Research Centre, Cairo, Egypt
  • Rania N Sabry Associate professor of Child Health, National Research Centre, Cairo, Egypt
  • Eman Awadallah Researcher of Clinical and Chemical Pathology department, National Research Centre, Cairo, Egypt



 Objective: Type 1 diabetes mellitus (T1DM) is a widespread metabolic disease, which frequently carries with it a significant impact on human health. Oxidative damage and tissue inflammation have been claimed to be a typical pathogenic component for the progression of diabetic complications. We aim in this study to explore the relation of urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) (as a marker of nucleic acid oxidation) to microvascular complications in T1DM.

Methods: A case–control study, enrolling 45 T1DM children and an equivalent number of healthy subjects, was performed. Full clinical examination and anthropometric measurement were performed to all subjects. Urinary assessment for 8-oxodG and albumin was done in addition to blood sampling for lipid profile and glycated Hb (HbA1c) assay. Complete ocular examination for assessment of diabetic retinopathy (DR) was also done.

Results: Levels of urinary 8-oxodG, serum cholesterol, triglycerides, and low-density lipoprotein in cases were significantly higher than non-diabetics; these levels were likewise higher in uncontrolled T1DM patients in comparison with well-controlled T1DM subjects. Urinary 8-oxodG and HbA1c were significantly higher in diabetic patients with albuminuria and DR compared to patients without complications. Significant positive correlation was found between 8-oxodG with HbA1c (r=0.8, p<0.01), diastolic blood pressure (DBP) (r=0.4, p=0.02), and cholesterol (r=0.4, p=0.05).

Conclusion: Urinary 8-oxodG was found to be a reliable marker for assessing oxidative DNA damage in T1DM and can be used in the determination of microvascular complications related to diabetes.

Keywords: Urinary 8-oxo-7, 8-dihydro-2′-deoxyguanosine, Type1 diabetes, DNA oxidation, Microvascular complications.


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How to Cite
El Wakeel, M. A., M. Abou-el-asrar, G. M. El-kassas, M. A. Elabd, D. Abu Zeid, R. N. Sabry, and E. Awadallah. “URINARY MARKERS OF OXIDATIVE DNA DAMAGE IN TYPE 1 DIABETIC CHILDREN: RELATION TO MICROVASCULAR COMPLICATIONS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 10, Sept. 2017, pp. 318-22, doi:10.22159/ajpcr.2017.v10i10.18930.
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