PHARMACOGNOSTICAL STANDARDIZATION OF RHIZOMES OF NUPHAR LUTEUM

  • Manpreet Kaur Department of Pharmaceutical Sciences (Pharmacognosy), G.H.G Khalsa College of Pharmacy, Gurusar Sadhar - 141 104, Ludhiana, Punjab, India.
  • Hayat M Mukhtar Department of Pharmaceutical Sciences (Pharmacognosy), Shaheed Bhagat Singh College of Pharmacy, Patti, Tarn Taran, Amritsar, Punjab. India

Abstract

Objective: This study provides the detailed summary of pharmacognostical characters of rhizomes to give clear standards for the identification ofdrug.

Methods: Important details such as macroscopy, microscopy (transverse section [TS] and powder microscopy) along with physico-chemicalparameters (such as loss on drying, ash values, and extractive values), and quantitative studies (i.e foreign matter, length and width of xylem vesselsand calcium oxalate crystals, and diameter of starch grains) were performed by standard procedure.

Results: The findings of macroscopic studies revealed that the rhizomes were internally creamish to light brown and dark brown externally withlongitudinal wrinkles. TS and powder microscopy of rhizomes showed the presence of fragments of cork, cellulosic parenchyma; scalariform typexylem vessels, fragments of ligneous parenchyma, lignified astrosclereids, and small ovoid, and rounded starch grains. The mean length and meanwidth of xylem vessels and calcium oxalate crystals were found to be 406 μm (micrometer) and 60.2 μm and 63.36 μm and 48.96 μm. Whereas meandiameter of starch grains was 34.56 μ. Physicochemical standards quantified were foreign organic matter (0.2%), total ash (12.3%), acid insolubleash (6.3%), water soluble ash (3%), loss on drying (12.5%), alcohol-soluble extractive value (32.6%), and water soluble extractive value (27.6%).

Conclusion: As various pharmacognostical and physico-chemical parameters have pivotal roles in authentication, identification and establishmentsof quality parameters of the plant. This study was conducted for this purpose.

Keywords: Nuphar luteum, Nymphaeaceae, Pharmacognostical, Physicochemical.

References

1. USDA. Germplasm Resources Information Network. Beltsville:Germplasm Resources Laboratory; 2002.
2. Ll’ inskaya TN, Kuzovkov AD, Monakhova TG. Alkaloids from Nuphar luteum. Chem Nat Compd 1967;3(3):147-9.
3. Brain KR, Turner TD. The Practical Evaluation of Phytopharmaceuticals. Bristol: Wright-Scientechnica; 1975.
4. Saini S, Dhiman A, Nanda S. Pharmacognostical and phytochemical studies of Piper betle Linn leaf. Int J Pharm Pharm Sci 2016;8(5):222-6.
5. Ali I, Rhizwani GH, Shreef H, Khan S. Pharmacognostic studies of Dalbergia sisoo Roxb. Int J Pharm Pharm Sci 2016;8(12):48-53.
6. Mukherjee PK. Morphological examinations. Quality Control of Herbal Drugs. New Delhi: Business Horizon’s Pharmaceutical Publishers;2002. p. 131-47.
7. Johansen DA. Plant Microtechnique. 1st ed. New York and London:McGraw-Hill Book Co., Inc.; 1940.
8. Anonymous. The Indian Pharmacopoeia. New Delhi: Government of India Publication; 1940.
9. Anonymous. Quality Control Methods for Medicinal Plant Materials.Geneva: Organization Mondiale De La Sante; 1996.
10. Evans WC. Morphological and microscopical examinations of drugs.Trease and Evans Pharmacognosy. 15th ed. London: Saunders Ltd.;
2003. p. 516-37.
11. Ali M. Text Book of Pharmacognosy. New Delhi: CBS Publishers & Distributers; 2008.
12. Maji JK, Janani H, Patel B. Standardisation of the anticonvulsant polyherbal formulation by pharmacognostical, DNA fingerprinting
and high-performance thin layer chromatography combined with chemometrics. Int J Curr Pharm Res 2017;9(1):50-8.
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Kaur, M., and H. M. Mukhtar. “PHARMACOGNOSTICAL STANDARDIZATION OF RHIZOMES OF NUPHAR LUTEUM”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 8, Aug. 2017, pp. 267-9, doi:10.22159/ajpcr.2017.v10i8.19030.
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