TRANSFERSOMAL GEL CONTAINING GREEN TEA (CAMELLIA SINENSIS L. KUNTZE) LEAVES EXTRACT: INCREASING IN VITRO PENETRATION
Objective: The aim of this study was to increase penetration of EGCG from green tea leaves extract (Camellia sinensis L. Kuntz) through the skin by formulating them into a transfersomal gel.
Methods: Transfersomes were prepared by thin-layer hydration method, with different concentration of the extract that equivalent to 1% (F1), 1.5% (F2), and 2% (F3) EGCG. Transfersomes formula with good characteristics would be incorporated into a gel formulation. A gel without transfersomes prepared as a control of comparison. Both of gels were evaluated their physicochemical properties. In vitro penetration test using Franz diffusion cell with the skin of female Sprague-Dawley rats was also performed.
Results: The results showed that F1 had the best physicochemical properties. F1 had a spherical shape, Dmean volume at 107.82Â±0.44 nm, polidispersity index at 0.07Â±0.01, zeta potential at -40.3Â±0.10 mV, and entrapment efficiency at 63.16Â±0.65%. Cumulative amount of EGCG penetrated from transfersomal and non-transfersomal gel were were 1302.63Â±20.67 Î¼g/cm2 and 414.86Â±4.40 Î¼g/cm2, resepctively (P<0.05). Flux penetration of transfersomal and non-transfersomal gel were was 57.594Â±0.91 Î¼g/cm2.h and 36.144Â±1.22 Î¼g/cm2.h, respectively.
Conclusion: It can be concluded that transfersomal gel could increase the in vitro penetration of EGCG from green tea leaves extract compared to non-transfersomal one.
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