TRANSFERSOMAL GEL CONTAINING GREEN TEA (CAMELLIA SINENSIS L. KUNTZE) LEAVES EXTRACT: INCREASING IN VITRO PENETRATION

  • Effionora Anwar Faculty of Pharmacy, Laboratory of Pharmaceutics and Pharmaceutical Technology, Universitas Indonesia, Depok, West Java, Indonesia.
  • Tahmida Diazputri Utami Faculty of Pharmacy, Laboratory of Pharmaceutics and Pharmaceutical Technology, Universitas Indonesia, Depok, West Java, Indonesia.
  • Delly Ramadon Faculty of Pharmacy, Laboratory of Pharmaceutics and Pharmaceutical Technology, Universitas Indonesia, Depok, West Java, Indonesia.

Abstract

Objective: The aim of this study was to increase penetration of EGCG from green tea leaves extract (Camellia sinensis L. Kuntz) through the skin by formulating them into a transfersomal gel.

Methods: Transfersomes were prepared by thin-layer hydration method, with different concentration of the extract that equivalent to 1% (F1), 1.5% (F2), and 2% (F3) EGCG. Transfersomes formula with good characteristics would be incorporated into a gel formulation. A gel without transfersomes prepared as a control of comparison. Both of gels were evaluated their physicochemical properties. In vitro penetration test using Franz diffusion cell with the skin of female Sprague-Dawley rats was also performed.

Results: The results showed that F1 had the best physicochemical properties. F1 had a spherical shape, Dmean volume at 107.82±0.44 nm, polidispersity index at 0.07±0.01, zeta potential at -40.3±0.10 mV, and entrapment efficiency at 63.16±0.65%. Cumulative amount of EGCG penetrated from transfersomal and non-transfersomal gel were were 1302.63±20.67 μg/cm2 and 414.86±4.40 μg/cm2, resepctively (P<0.05). Flux penetration of transfersomal and non-transfersomal gel were was 57.594±0.91 μg/cm2.h and 36.144±1.22 μg/cm2.h, respectively.

Conclusion: It can be concluded that transfersomal gel could increase the in vitro penetration of EGCG from green tea leaves extract compared to non-transfersomal one.

 

Keywords: Epigallocatechin gallate, Green tea leaves extract, In vitro penetration test, Transfersomes, Transfersomal gel.

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Anwar, E., T. D. Utami, and D. Ramadon. “TRANSFERSOMAL GEL CONTAINING GREEN TEA (CAMELLIA SINENSIS L. KUNTZE) LEAVES EXTRACT: INCREASING IN VITRO PENETRATION”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 8, Aug. 2017, pp. 294-8, doi:10.22159/ajpcr.2017.v10i8.19124.
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