• Thirumurugan D Department of Biotechnology, Faculty of Science and Humanities, SRM University, Chennai, Tamil Nadu, India.
  • Ibrahim Adamu Karfi Department of Microbiology, Kano University of Science and Technology Wudil, Nigeria.
  • Vijayakumar R Department of Microbiology, Bharathidasan University Constituent College, Perambalur, Tiruchirappalli, Tamil Nadu, India.
  • Nithya Tg Department of Biotechnology.



 Objective: The present study is conducted to investigate the abilities of microorganisms to degrade heavy metals in industrial tannery effluent sample.

Methods: Tannery effluent sample was collected from effluent treatment plant and analyzed for physicochemical parameters. The potential microbes were isolated and identified by morphological and biochemical characterization. The sample was analyzed before and after to assess the heavy metal reducing the ability of the microorganism and the respective percentage of reduction were studied using X-ray fluorescence spectrometry.

Results: The samples were initially found to be highly contaminated with chromium, nickel, and cadmium. Out of three potential isolates, the isolate Streptomyces sp. was found to exhibit a better reduction against chromium (25.7%), cadmium (14.6%), and nickel (23.1%) in 50 ppm at longer incubation period. Comparatively, the reduction abilities of all the three isolates against all the three heavy metals increased with the increase in the incubation period but decreased with the increase in initial metal ion concentration except in the case of Streptomyces sp. against nickel where the reducing ability increased with the increase in metal concentration.

Conclusion: Apparently, the present study revealed that Streptomyces sp. had a better remediation potential than the indigenous Pseudomonas sp. and Aspergillus sp. Ultimately, the finding of this research has shown that the Streptomyces sp. can be used as a potent bioremediation agent for treating tannery and industrial effluent in an eco-friendly process.

Keywords: Bioremediation, Tannery effluent, Heavy metals, Streptomyces sp.

Author Biography

Thirumurugan D, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Chennai, Tamil Nadu, India.
Assistant Professor Dept. of Biotechnology


1. Tariq SR, Shah MH, Shaheen N, Khalique A, Manzoor S, Jaffar M. Multivariate analysis of selected metals in tannery effluents and related soil. J Hazard Mater 2005;122(1-2):17-22.
2. McMullan G, Meehan C, Conneely A, Kirby N, Robinson T, Nigam P, et al. Microbial decolourisation and degradation of textile dyes. Appl Microbiol Biotechnol 2001;56(1-2):81-7.
3. Apte AD, Verma S, Tare V, Bose P. Oxidation of Cr(III) in tannery sludge to Cr(VI): Field observations and theoretical assessment. J Hazard Mater 2005;121(1-3):215-22.
4. Bruins MR, Kapil S, Oehime FW. Microbial resistance to metals in the environment. Ecotoxicol Environ Saf 2000;45(3):198-207.
5. Hare L. Aquatic insects and trace metals: Bioavailability, bioaccumulation, and toxicity. Crit Rev Toxicol 1992;22(5-6):327-69.
6. Lenart A, Wolny-Koladka K. The effect of heavy metal concentration and soil pH on the abundance of selected microbial groups within ArcelorMittal Poland steelworks in Cracow. Bull Environ Contam Toxicol 2013;90(1):85-90.
7. Chibuike GU, Obiora SC. Heavy metal polluted soils: Effect on plants and bioremediation methods. Appl Environ Soil Sci 2014;14:1-12.
8. Chaudry MA, Ahmad S, Malik MT. Supported liquid membrane technique applicability for removal of chromium from tannery wastes. Waste Manage 1997;17(4):211-8.
9. Sarkar B. Heavy Metals in the Environment. New York: Marcel Dekker; 2002.
10. Leena R, Raj DS. Biodecolourization of textile effluent containing reactive back-B by effluent adapted and non adapted bacteria. Afr J Biotechnol 2008;7(18):3309-13.
11. Olukanni OO, Osuntoki AA, Gabriele GO. Textile effluent biodegradation potentials of textiles effluent-adapted and non-adapted bacteria. Afr J Biotechnol 2006;5:80-4.
12. Thirumurugan D, Sankari D, Vijayakumar R. Screening of chitinase production and antifungal activity of Streptomyces sp. Act7 from East Coast Region, South India. Int J Pharm Pharm Sci 2015;7(5):38-41.
13. Chen KC, Huang WT, Wu JY, Houng JY. Microbial decolorization of azo dyes by Proteus mirabilis. J Ind Microbiol Biotechnol 1999;23(1):686-90.
14. Puvaneswari N, Muthukrishnan J, Gunasekaran P. Toxicity assessment and microbial degradation of azo dyes. Indian J Exp Biol 2006;44(8):618-26.
15. American Public Health Association. Standard Methods for the Examination of Water and Waste Water. 20th ed. New York, Washington, DC: American Public Health Association; 1998.
16. Zarazua G, Giron-Romero K, Tejeda S, Leon CC, Avila-Perez P. Total reflection x-ray fluorescence analysis of toxic metals in fish tissues. Am J Anal Chem 2014;5:805-11.
17. Christopher M, Cournoyer B, Nesma X. Novel telluride amended media and specific chromosomal and Ti plasmid probes for direct analysis of soil populations of Agrobacterium biovar 1 and 2. Appl Environ Microbiol 2001;67:65-74.
18. Thirumurugan D, Vijayakumar R. Investigation of physico-chemical properties of rhizosphere sediments from East Coast region, Tamil Nadu, India. Int J Pharm Pharm Sci 2015;8(2):332-4.
19. Banat IM, Nigam P, Singh D, Marchant R. Microbial decolorization of textile dye containing effluents: A review. Bioresour Technol 1996;58(3):217-27.
20. Chowdhury M, Mostafa MG. Treatment of leather industrial effluents by filtration and coagulation processes. Water Resour Ind 2013;3:11-22.
21. Panda SS, Sahoo K, Muduli SD, Sahoo G, Ahemad MD, Nayak BB, et al. Chromium tolerant indigenous fungal strains from industrial effluents of Anugul district, Odisha. J Biol Life Sci 2014;2(2):634-40.
22. Deepali D. Bioremediation of chromium (Vi) from textile industry’s effluent and contaminated soil using Pseudomonas putida. Iran J Energy Environ 2011;2(1):24-31.
23. Nagamani A, Kunwar IK, Manoharachary C. Handbook of Soil Fungi. New Delhi: I K International Publishing House; 2006.
24. Karmakar M, Ray RR. Characterization of hexavalent chromium reducing bacterial strain isolated from tannery effluents of Kolkata. Int J Pharm Biol Arch 2013;4:337-41.
25. Han R, Li F, Liu T, Li X, Wu Y, Wang Y, et al. Effects of incubation conditions on Cr(VI) reduction by c-type cytochromes in intact Shewanella oneidensis MR-1 cells. Front Microbiol 2016;7:732-46.
26. El Baz S, Baz M, Barakate M, Hassani L, El Gharmali A, Imziln B. Resistance to and accumulation of heavy metals by actinobacteria isolated from abandoned mining areas. Sci World J 2015;15:1-12.
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How to Cite
D, T., I. Adamu Karfi, V. R, and N. Tg. “AN ALTERNATIVE APPROACH ON BIOREMEDIATION OF HEAVY METALS IN TANNERY EFFLUENTS WASTE USING STREPTOMYCES SP.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 10, Sept. 2017, pp. 323-6, doi:10.22159/ajpcr.2017.v10i10.19480.
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