ANTI-INFLAMMATORY ACTIVITY OF SYZYGIUM AROMATICUM SILVER NANOPARTICLES: IN VITRO AND IN SILICO STUDY
Keywords:Syzygium aromaticum, Antioxidant, Anti-inflammatory, Interleukin-1 beta, Molecular docking
Â Objective: In the present study, antioxidant and anti-inflammatory activity of Syzygium aromaticum (clove) silver nanoparticles (clove AgNP's) was evaluated.
Methods: Gas chromatography-mass spectrometry technique was used to identify the compounds present in the aqueous extract of clove. Fourier transform infrared (FT-IR) analysis was done to characterize the clove silver AgNP's. 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was performed to evaluate the antioxidant property of nanoparticles (0.05 and 0.25 mg/ml) and aqueous extracts (0.05, 0.1, and 0.25 mg/ml) of clove. The anti-inflammatory property of the clove AgNP's was determined by inhibition of protein denaturation and downregulation of interleukin-1 beta. In silico molecular docking studies was performed using Schrodinger Maestro software.
Results: Eugenol was found to be highest with 16.27%. The AgNP's exhibited dose-dependent antioxidant property. AgNP's scavenged 80% of radical at the concentration of 0.25 mg/ml. The scavenging activity of AgNP's markedly increased when compared to aqueous extract at the same concentration. Inhibition of protein denaturation assay also revealed AgNP's showing the highest activity (66%) when compared with drug aspirin (55%) and aqueous extract (56%). In the enzyme-linked immunosorbent assay (ELISA) method, AgNP's showed better inhibition (80%) when compared to aqueous extract (60%). Among 15 compounds, two compounds (eugenol and methyl 14-methylpentadecanoate) showed good glide energy, docking score, and hydrogen-bonded active site interactions with the protein interleukin-1 beta.
Conclusion: As AgNP's were more active than the aqueous extract, it may be considered for pharmacological activity against inflammatory disorders.
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