ANTI-INFLAMMATORY ACTIVITY OF SYZYGIUM AROMATICUM SILVER NANOPARTICLES: IN VITRO AND IN SILICO STUDY


Reslee Elsa Varghese, Ragavan D, Saranya Sivaraj, Dasararaju Gayathri, Gomathi Kannayiram

Abstract


 

 Objective: In the present study, antioxidant and anti-inflammatory activity of Syzygium aromaticum (clove) silver nanoparticles (clove AgNP’s) was evaluated.

Methods: Gas chromatography-mass spectrometry technique was used to identify the compounds present in the aqueous extract of clove. Fourier transform infrared (FT-IR) analysis was done to characterize the clove silver AgNP’s. 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was performed to evaluate the antioxidant property of nanoparticles (0.05 and 0.25 mg/ml) and aqueous extracts (0.05, 0.1, and 0.25 mg/ml) of clove. The anti-inflammatory property of the clove AgNP’s was determined by inhibition of protein denaturation and downregulation of interleukin-1 beta. In silico molecular docking studies was performed using Schrodinger Maestro software.

Results: Eugenol was found to be highest with 16.27%. The AgNP’s exhibited dose-dependent antioxidant property. AgNP’s scavenged 80% of radical at the concentration of 0.25 mg/ml. The scavenging activity of AgNP’s markedly increased when compared to aqueous extract at the same concentration. Inhibition of protein denaturation assay also revealed AgNP’s showing the highest activity (66%) when compared with drug aspirin (55%) and aqueous extract (56%). In the enzyme-linked immunosorbent assay (ELISA) method, AgNP’s showed better inhibition (80%) when compared to aqueous extract (60%). Among 15 compounds, two compounds (eugenol and methyl 14-methylpentadecanoate) showed good glide energy, docking score, and hydrogen-bonded active site interactions with the protein interleukin-1 beta.

Conclusion: As AgNP’s were more active than the aqueous extract, it may be considered for pharmacological activity against inflammatory disorders.


Keywords


Syzygium aromaticum, Antioxidant, Anti-inflammatory, Interleukin-1 beta, Molecular docking.

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About this article

Title

ANTI-INFLAMMATORY ACTIVITY OF SYZYGIUM AROMATICUM SILVER NANOPARTICLES: IN VITRO AND IN SILICO STUDY

Keywords

Syzygium aromaticum, Antioxidant, Anti-inflammatory, Interleukin-1 beta, Molecular docking.

DOI

10.22159/ajpcr.2017.v10i11.19904

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 11 November 2017 Page: 370-373

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

157 Views | 203 Downloads

Authors & Affiliations

Reslee Elsa Varghese
Department of Biotechnology, Dr. M.G.R Educational and Research Institute University, Chennai, Tamil Nadu, India
India

Ragavan D
Department of Biotechnology, Dr. M.G.R Educational and Research Institute University, Chennai, Tamil Nadu, India
India

Saranya Sivaraj
Department of Biotechnology, Dr. M.G.R Educational and Research Institute University, Chennai, Tamil Nadu, India
India

Dasararaju Gayathri
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, Tamil Nadu, India.
India

Gomathi Kannayiram
Department of Biotechnology, Dr. M.G.R Educational and Research Institute University, Chennai, Tamil Nadu, India
India


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