ANTI-INFLAMMATORY ACTIVITY OF NIGELLA SATIVA SILVER NANOPARTICLES: BIOCHEMICAL STUDY

  • GOMATHI KANNAYIRAM Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India. http://orcid.org/0000-0003-0814-3383
  • SANDHYA A Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India.
  • SOWMIYA S Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India.
  • VALARMATHI S Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India.
  • DANIEL JOSEPH Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India.

Abstract

Objective: The aim of this study is to evaluate the anti-inflammatory activity of Nigella sativa silver nanoparticles (NS AgNPs).


Methods: Fourier transform infrared analysis was used to characterize the NS AgNPs and the extract. 2,2-diphenylpicrylhydrazyl assay was done to test the antioxidant potency of NS AgNP. Furthermore, in vitro anti-inflammatory activity of the extract and the NS AgNP was determined by red blood cell (RBC) membrane stabilization assay, protein inhibition assay, and interleukin-1 (IL-1) beta assay.


Results: The NS AgNP exhibited dose-dependent antioxidant property. At the concentration 0.01 mg/ml 80% of radical was scavenged by NS AgNP. Inhibition of protein denaturation assay also suggests that NS AgNP shows the highest activity (70%) when compared with the standard drug aspirin (65%). RBC assay suggests that NS AgNP stabilizes the RBC membrane and prevents leaking. In the enzyme-linked immunosorbent assay method the NS AgNP showed better IL-1 beta inhibition activity when compared to aqueous extract.


Conclusion: From the study, it was inferred that NS AgNPs are more effective when compared to the extract. These results suggest that NS AgNP can be used to treat inflammatory disorders.

Keywords: Nigella sativa, Fourier transform infrared, Nanoparticles, Protein denaturation, Enzyme-linked immunosorbent assay.

Author Biography

GOMATHI KANNAYIRAM, Department of Biotechnology, Dr. M.G.R Educational and Research Institute, University, Chennai - 600 095, Tamil Nadu, India.

DEPARTMENT OF BIOTECHNOLOGY DR. M.G.R.EDUCATIONAL AND RESEARCH INSTITUTE

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KANNAYIRAM, G., S. A, S. S, V. S, and D. JOSEPH. “ANTI-INFLAMMATORY ACTIVITY OF NIGELLA SATIVA SILVER NANOPARTICLES: BIOCHEMICAL STUDY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 2, Jan. 2019, pp. 346-9, https://innovareacademics.in/journals/index.php/ajpcr/article/view/29775.
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