ANTI-INFLAMMATORY ACTIVITY OF NIGELLA SATIVA SILVER NANOPARTICLES: BIOCHEMICAL STUDY
Keywords:Nigella sativa, Fourier transform infrared, Nanoparticles, Protein denaturation, Enzyme-linked immunosorbent assay
Objective: The aim of this study is to evaluate the anti-inflammatory activity of Nigella sativa silver nanoparticles (NS AgNPs).
Methods: Fourier transform infrared analysis was used to characterize the NS AgNPs and the extract. 2,2-diphenylpicrylhydrazyl assay was done to test the antioxidant potency of NS AgNP. Furthermore, in vitro anti-inflammatory activity of the extract and the NS AgNP was determined by red blood cell (RBC) membrane stabilization assay, protein inhibition assay, and interleukin-1 (IL-1) beta assay.
Results: The NS AgNP exhibited dose-dependent antioxidant property. At the concentration 0.01 mg/ml 80% of radical was scavenged by NS AgNP. Inhibition of protein denaturation assay also suggests that NS AgNP shows the highest activity (70%) when compared with the standard drug aspirin (65%). RBC assay suggests that NS AgNP stabilizes the RBC membrane and prevents leaking. In the enzyme-linked immunosorbent assay method the NS AgNP showed better IL-1 beta inhibition activity when compared to aqueous extract.
Conclusion: From the study, it was inferred that NS AgNPs are more effective when compared to the extract. These results suggest that NS AgNP can be used to treat inflammatory disorders.
Baizabal-Aguirre VM, Rosales C, López-Macías C, Gómez MI. Control and resolution mechanisms of the inflammatory response 2016. Mediators Inflamm 2016;2016:3591797.
Raghavan R, Cheriyamundath S, Madassery J. Exploring the mechanisms of cytotoxic and anti-inflammatory property of andrographolide and its derivates. Pharmacogn Rev 2016;12:56-65.
Verma S. Medicinal plants with anti-inflammatory activity. J Phytopharmacol 2016;5:157-9.
Ghasemian M, Owali S, Owlia MB. Review of anti-inflammatory herbal medicines. Adv Pharmacol Sci 2016;2016:1-11.
Al-Ghamdi MS. The anti-inflammatory, analgesic and antipyretic activity of Nigella sativa. J Ethnopharmacol 2001;76:45-8.
Deepa M, Renuka D. Potential anti-inflmmatory medicinal plants-a review. Int J Pharm Pharm Sci 2014;6:43-9.
Rakesh W, Saranya S, Gurukarthikeyan, Kumar PR, Lokeshmari SS, Gayathri D, et al. Chitosan gallic acid microsphere incorporated collagen matrix for chronic wounds: Biophysical and biochemical characterization. Int J Pharm Pharm Sci 2014;6:94-100.
Mary EJ, Inbathamizh L. Green synthesis and characterization of nano silver using leaf extract of Morinda pubescens. Asian J Pharm Clin Res 2012;5:159-62.
Varghese RE, Raghavan D, Sivaraj S, Gayathri D, Kannayiram G. Anti-inflammatory activity of Syzgium aromaticum silver nanoparticles: In vitro and in silico study. Asian J Pharm Clin Res 2017;10:370-3.
Farid R, Day T, Friesner RA, Pearlstein RA. New insights about HERG blockade obtained from protein modeling, potential energy mapping, and docking studies. Bioorg Med Chem 2006;14:3160-73.
Quillin ML, Wingfield PT, Matthews BW. Determination of solvent content in cavities in IL-1beta using experimentally phased electron density. Proc Natl Acad Sci U S A 2006;103:19749-53.
Leelaprakash G, Dass SM. In vitro anti-inflammatory activity of methanol extract of Enicostemma axillare. Int J Drug Dev Res 2011;3:189-96.
Pisoschi AM, Negulescu GP. Methods for total antioxidant activity determination: A review. Biochem Anal Biochem 2011;1:106.
Ansari AQ, Ahmed SA, Waheed MA, Juned AS. Extraction and determination of antioxidant activity of Withania somnifera Dunal. Eur J Exp Biol 2013;3:502-7.
Neurath H. Proteolytic processing and physiological regulation. Trends Biochem Sci 1989;14:268-71.
Bang JS, Oh DH, Choi HM, Sur BJ, Lim SJ, Kim JY, et al. Anti-inflammatory and antiarthritic effects of piperine in human interleukin 1beta-stimulated fibroblast-like synoviocytes and in rat arthritis models. Arthritis Res Ther 2009;11:R49.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.