MOLECULAR IDENTIFICATION OF PROBIOTIC YEAST STRAINS AND THEIR CHARACTERIZATION
The objective of the present study was to identify the potential yeast isolates at themolecular level and evaluate their probiotic characteristics.
Molecular characterization was done for 5 potential probiotic yeast strains. In vitro assays have been conducted to evaluate the probiotic properties such as NaCl tolerance, autoaggregation and co-aggregation. Haemolyticactivity, urease activity and cytotoxicity tests were carried out for safety assay during the characterization of yeast strains.
In this study, the yeast strains viz. LM,MR,GOI,GII2and WI were identified at molecular level and named as Yarrowialipolytica VIT-MN01, Kluyveromyces lactis VIT-MN02, Lipomyces starkeyi VIT-MN03, Saccharomycopsis fibuligera VIT-MN04, Brettanomyces custersianus VIT-MN05 respectively. Maximum autoaggregation and coaggregationwere noted in case of Lipomyces starkeyi VIT-MN03, Saccharomycopsis fibuligeraVIT-MN04, and Brettanomyces custersianus VIT-MN05. In vitro toxicity assay was performed and all the yeast strains showednon-toxic nature.
Five yeast strains have been studied for their probiotic characteristics and identified at molecular level. Out offive yeast strains, three strains showed maximum adhesion ability,which is a prerequisite for colonization and protection of gastrointestinal tract. All the yeast strains are validated as a safe bioresources because of their non - hemolytic activities and non-production of urease. It can be concluded that the identified yeast strainscan serve as promising probiotics in various fields offood industry.
2. Gueimonde M, Salminen S. New methods for selecting and evaluating probiotics. Dig Liver Dis 2006;38 Suppl 2:S242-7.
3. Czerucka D, Piche T, Rampal P. Review article: Yeast as probiotics-Saccharomyces boulardii. Aliment Pharmacol Ther 2007;26(6):767-78. 4. Kumura H, Tanoue Y, Tsukahara M, Tanaka T, Shimazaki K. Screening of dairy yeast strains for probiotic applications. J Dairy Sci 2004;87(12):4050-6.
5. Kos B, Suskovic J, Vukovic S, Simpraga M, Frece J, Matosic S. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J Appl Microbiol 2003;94(6):981-7.
6. Muller JA, Ross RP, Fitzgerald GF, Stanton C. Manufacture of probiotic Bacteria. Prebiotics Probiotics Science and Technology. New York: Springer; 2009. p. 725-59.
7. Joint FAO/WHO Expert Committee on Food Additives. Meeting and World Health Organization. JECFA; 2002. p. 56.
8. Ragavan ML, Das N. Isolation and characterization of potential probiotic yeast from different sources. Asian J Pharm Clin Res 2017;10(4):451-5.
9. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990;215(3):403-10.
10. Joseph F. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 1985;39(4):783-91.
11. Lohith K, Anu A. In vitro probiotic characterization of yeasts of food and environmental origin. Int Probiotics Prebiotics 2014;9(3):1-6.
12. Jankovic T, Frece J, Abram M, Gobin I. Aggregation ability of potential probiotic Lactobacillus plantarum strains. Int J Sci Eng Res 2012;6:19-24.
13. Manns JM, Mosser DM, Buckley HR. Production of a hemolytic factor by Candida albicans. Infect Immun 1994;62(11):5154-6.
14. Prabhurajeshwar C, Chandrakanth KR. Development of in vitro methodologies for inhibition of pathogenic Bacteria by potential probiotic Lactobacillus sps; An evidence for production of antimicrobial substances. Int J Pharm Pharm Sci 2016;8(12):277-86.
15. Bharathi N, Meyyappan RM. Production of urease enzyme from ureolytic yeast cell. Int J Eng Res Gen Sci 2015;3(2):643-7.
16. Joshi N, Shanmugam T, Kaviratna A, Banerjee R. Proapoptotic lipid nanovesicles: synergism with paclitaxel in human lung adenocarcinoma A549 cells. J Control Release 2011;156(3):413-20.
17. Mohanty D, Saini MR, Mohapatra S. In vitro study on release of bioactive antimicrobial compounds from dairy products by certain promising probiotic Lactobacillus strains. Int J Pharm Pharm Sci 2017;9(4):27-31.
18. Collado MC, Meriluoto J, Salminen S. Adhesion and aggregation properties of probiotic and pathogen strains. East Afr Med J 2008;226(5):1065-73.
19. Del Re B, Sgorbati B, Miglioli M, Palenzona D. Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Lett Appl Microbiol 2000;31(6):438-42.
20. VÃ©lez MP, De Keersmaecker SC, Vanderleyden J. Adherence factors of Lactobacillus in the human gastrointestinal tract. FEMS Microbiol Lett 2007;276(2):140-8.
21. Mora D, Arioli S. Microbial urease in health and disease. PLoS Pathog 2014;10(12):e1004472.
22. Clemedson C, Ekwall B. Overview of the final MEIC results: I. The in vitro-in vitro evaluation. Toxicol In Vitro 1999;13(4-5):657-63.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.