IN VITRO CYTOTOXIC AND APOPTOTIC EFFECT OF PASSIFLORA FOETIDA AGAINST CERVICAL CANCER CELLS AND ITS FOURIER TRANSFORM INFRARED PROFILING


Dheeban Shankar, Basker S, Karthik S

Abstract


 

 Objective: This study was aimed on the analysis of cytotoxic and apoptotic action of Passiflora foetida followed by identification of the functional groups responsible for the activity.

Methods: In this study, cytotoxic and apoptotic effect of methanol extract of P. foetida were analyzed by treating HeLa cell line cultures with different concentrations of the extract (25, 50, 75, 100, and 125 μg/ml), and thereby the activity was ratified by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and propidium iodide staining. The functional groups of the bioactive compounds for the effectiveness of the treatment were known by Fourier transform infrared spectroscopy analysis (FTIR).

Results: The cytotoxic activity was found to be increased in a dose-dependent manner with inhibitory concentration value of 21.55 μg/ml and showed an effective apoptosis. Further, FTIR analysis confirmed the presence of functional groups of alkaloids, flavonoids, saponins, steroids, terpenoids, phenols and cardiac glycosides which might be responsible for the aforesaid activity.

Conclusion: The cytotoxic and apoptotic action of P. foetida was proved to be very effective, and the tenable functional groups were identified.


Keywords


Passiflora foetida, Cytotoxic, Apoptotic, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Propidium iodide.

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About this article

Title

IN VITRO CYTOTOXIC AND APOPTOTIC EFFECT OF PASSIFLORA FOETIDA AGAINST CERVICAL CANCER CELLS AND ITS FOURIER TRANSFORM INFRARED PROFILING

Topics

Biotechnology

Keywords

Passiflora foetida, Cytotoxic, Apoptotic, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Propidium iodide.

DOI

10.22159/ajpcr.2017.v10i11.20226

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 11 November 2017 Page: 46-49

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

48 Views | 80 Downloads

Authors & Affiliations

Dheeban Shankar
Research and Development Centre, Bharathiar University, Coimbatore, Tamil Nadu, India
India

Basker S
Department of Biotechnology, Nandha Arts and Science College, Erode, Tamil Nadu, India.
India

Karthik S
Department of Botany, Government Arts College (Autonomous), Salem, Tamil Nadu, India.
India


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