EARLY DIAGNOSIS OF SEPSIS THROUGH SEPSIS MARKERS AND SEPSIS INDEX THROUGH FLOW CYTOMETRY TECHNOLOGY


Sima Chouhan, Jagadish Hansa, Sarita Pradhan

Abstract


 

 Objective: Sepsis is a major medical challenge, and remains a burden by showing minimal symptoms, non-specific manifestation which leads to the high-mortality rate. The major cause of high mortality rate is due to the diagnostic pitfalls. The sepsis mechanism involves immune suppression associated with multiorgan dysfunction, uncontrolled infection, and death. This study aims to use new parameters in diagnosis of sepsis particularly neutrophil CD64 (nCD64), monocyte human leukocyte antigen-DR (mHLA-DR), performed by flow cytometer.

Methods: Available diagnostic tools such as blood culture (gold standard) and other tools are time-consuming which leads to death because the treatment is not commenced promptly due to unavailability of quick and accurate diagnostic procedures. To overcome such instances, there are different specific cell surface markers which are introduced to reveal sepsis at the earliest using flow cytometry technique. The newer technique allows determination of different cellular and functional pathological components of sepsis. Using this technology, a newer treatment modality (granulocyte macrophage colony-stimulating factor) can be used to reverse sepsis associated immune suppression.

Results: The results suggested that the flow cytometric evaluation of nCD64, mHLA-DR expression assay, seems to be promising as comparison to other tools available for diagnosis.

Conclusion: The pilot data suggest that the flow assay, “sepsis index” is a useful assay for diagnosing and discriminating sepsis in adult at intensive care unit settings in comparison to all the available current modalities of sepsis diagnosis. However, there are no other comparable parameters to diagnose early sepsis.


Keywords


Sepsis, Flow cytometry, Sepsis index.

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About this article

Title

EARLY DIAGNOSIS OF SEPSIS THROUGH SEPSIS MARKERS AND SEPSIS INDEX THROUGH FLOW CYTOMETRY TECHNOLOGY

Keywords

Sepsis, Flow cytometry, Sepsis index.

DOI

10.22159/ajpcr.2017.v10i11.20640

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 11 November 2017 Page: 145-148

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

37 Views | 39 Downloads

Authors & Affiliations

Sima Chouhan
Department of Pathology, Institute of Medical Sciences & SUM Hospital, Siksha O Anusandhan University, Bhubaneswar - 751 003, Odisha, India.
India

Jagadish Hansa
Directorate of Medical Research, Institute of Medical Sciences & SUM Hospital, Siksha O Anusandhan University, Bhubaneswar - 751 003, Odisha, India.
India

Sarita Pradhan
Department of Pathology, Institute of Medical Sciences & SUM Hospital, Siksha O Anusandhan University, Bhubaneswar - 751 003, Odisha, India.


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