• Prabhakaran D Department of Chemistry Periyar E.V.R. College (Autonomous), Trichy, Tamil Nadu, India
  • Senthamilselvi Mm Department of Higher Education, Joint Director of Collegiate Education, Tiruchirappalli, Tamil Nadu, India.
  • Rajeshkanna A Department of Chemistry, Periyar E. V. R. College (Autonomous), Tiruchirappalli, Tamil Nadu, India.



 Objective: To explore the anticancer activity of the flowers of Argemone mexicana L. against the human hepatoma cell line (HepG2).

Methods: In vitro anticancer activity was carried out to screen cytotoxicity effectiveness of the solid obtained from ethyl acetate fraction of A. mexicana L. flower extract at different concentrations against the HepG2 cell line. The MTT (methylthiazolyl diphenyl- tetrazolium bromide) assay for cell viability and markers is expected to confirm the cytotoxicity.

Result: Ethyl acetate fraction from the flower extract of A. mexicana L. was tested for its anticancer activity against HepG2 cell lines (liver cancer) at various concentrations by MTT assay. It was confirmed that the IC50 value of this sample was 72±1.7 μg/ml against liver cancer HepG2 cell line.

Conclusions: A. mexicana L. is a potential plant with anticancer activity. The isolation of the pure compounds and determination of the structure of individual compounds will be further performed.

Keywords: Argemone mexicana L., Anticancer activity, MTT assay, HepG2, Cytotoxicity.


1. Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 2015;136(5):E359-86.
2. Fukahori S, Yano H, Akiba J, Ogasawara S, Momosaki S, Sanada S, et al. Fucoidan, a major component of brown seaweed, prohibits the growth of human cancer cell lines in vitro. Mol Med Rep 2008;1(4):537-42.
3. Smyrniotopoulos V, Vagias C, Bruyère C, Lamoral-Theys D, Kiss R, Roussis V. Structure and in vitro antitumor activity evaluation of brominated diterpenes from the red alga Sphaerococcus coronopifolius. Bioorg Med Chem 2010;18(3):1321-30.
4. The Fact Sheet of World Health Organisation, February; 2017.
5. Tan W, Lu J, Huang M, Li Y, Chen M, Wu G, et al. Anti-cancer natural products isolated from Chinese medicinal herbs. Chin Med 2011;6(27):1-15.
6. Alonso-Castro AJ, Villarreal ML, Salazar-Olivo LA, Gomez-Sanchez M, Dominguez F, Garcia-Carranca A. Mexican medicinal plants used for cancer treatment: Pharmacological, phytochemical and ethnobotanical studies. J Ethnopharmacol 2011;133(3):945-72.
7. Ma X, Wang Z. Anticancer drug discovery in the future: An evolutionary perspective. Drug Discov Today 2009;14:1136-42.
8. Gumenyuk VG, Bashmakova NV, Kutovyy SY, Yashchuk VM, Zaika LA. Binding parameter of alkaloids berberine and sanguinarine with DNA. Ukr J Phys 2011;56(6):524-33.
9. Kim J, Park EJ. Cytotoxic anticancer candidates from natural resources. Curr Med Chem Anticancer Agents 2002;2(4):485-537.
10. Mann J. Natural products in cancer chemotherapy: Past, present and future. Nat Rev Cancer 2002;2(2):143-8.
11. Sithranga Boopathy N, Kathiresan K. Anticancer drugs from marine flora: An overview. J Oncol 2010;2010:214186.
12. Mukherjee A, Namahata D. Medicinal plant lore of the tribals of Sundargarh district, Orissa. Ethnobotany 1990;1(2):57-60.
13. Das PK, Misra MK. Some medicinal plants used by the tribals of Deomali and adjacent areas of Koraput district, Orissa. Indian J Forestry 1987;10:301-3.
14. Ibrahim HA, Ibrahim H. Phytochemical screening and toxicity evaluation on the leaves of Argemone mexicana Linn. (Papaveraceae). Int J Appl Sci 2009;3:39-43.
15. Bhattacharjee I, Chatterjee SK, Chatterjee S, Chandra G. Antibacterial potential of Argemone mexicana solvent extracts against some pathogenic bacteria. Mem Inst Oswaldo Cruze Rio de Janeiro 2006;101(6):645-8.
16. Chopra PN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants (Including the Supplement). New Delhi: Council of Scientific and Industrial Research; 1986.
17. Prusti AB, Mishra A. Interesting medico-botanical claims by Khouds of Nayagarh district of Orissa. Plant Sci Res 2005;27(182):16-23.
18. Alagesaboopathi C. Ethnomedicinal plants and their utilization by villagers in Kumaragiri Hills of Salem district of Tamilnadu, India. Afr J Tradit Complement Altern Med 2009;6(3):222-7.
19. Das GK, Murthy PN. Evaluation of Argemone mexicana Linn. Leaves for wound healing activity. J Nat Prod Plant Resour 2011;1(1):46-56.
20. Deepa M, Darsan MB, Ramalingam C. In vitro evaluation of the antioxidant, anti-inflammatory and antiproliferarive activities of the leaf extracts of Excoecaria agallocha L. Int J Pharm Pharm Sci 2015;7(11):346-52.
21. Priya PV, Radhika K, Kumar RS, Gowda BB, Beevi SS, Devi YP, et al. In vitro anti-cancer activity of aqueous and acetone extracts of Tridax procumbens leaf on PC 3 cell lines. Int J Pharm Pharm Sci 2011;3(4):1-4.
22. Vinceti M, Dennert G, Crespi CM, Zwahlen M, Brinkman M, Zeegers MP, et al. Selenium for preventing cancer. Cochrane Database Syst Rev 2014;3:CD005195.
23. Pais R, Dumitraşcu DL. “Do antioxidants prevent colorectal cancer? A meta-analysis”. Rom J Intern Med 2013;51:152-63.
24. Cortés-Jofré M, Rueda JR, Corsini-Muñoz G, Fonseca-Cortés C, Caraballoso M, Bonfill Cosp X. Drugs for preventing lung cancer in healthy people. Cochrane Database Syst Rev 2012;10:CD002141.
25. Ovadje P, Roma A, Steckle M, Nicoletti L, Arnason JT, Pandey S. Advances in the research and development of natural health products as main stream cancer therapeutics, Hindawi publishing corporation. Evid Based Complement Alternat Med 2015;751348:1-12.
26. Balachandran P, Govindarajan R. Cancer-an ayurvedic perspective. Pharmacol Res 2005;51(1):19-30.
27. Aggarwal BB, Prasad S, Reuter S, Kannappan R, Yadev VR, Park B, et al. Identification of novel anti-inflammatory agents from Ayurvedic medicine for prevention of chronic diseases: “Reverse pharmacology” and “bedside to bench” approach. Curr Drug Targets 2011;12(11):1595-653.
28. Perumal P, Sekar V, Rajesh V, Gandhimathi S, Kumar RS, Nazimudin KH. In vitro antioxidant activity of Argemone mexicana roots. Int J PharmTech Res 2010;2(2):1477-82.
29. Goswami M, Yadav A, Bhardwaj R, Joshi YC, Sharma RA. Antioxidant properties of methanolic extract of Argemone mexicana. Res J Med Plant 2014;8(4):167-77.
30. Shabana MM, Salama MM, Shahira M, Ismail LR. In vitro and in vivo anticancer activity of the fruit peels of Solanum melongena L. Against hepatocellular carcinoma. J Carcinog Mutagen 2013;4(3):1-6.
31. Leyva-Peralta MA, Robles-Zepeda RE, Garibay-Escobar A, Ruiz-Bustos E, Alvarez-Berber LP, Gálvez-Ruiz JC. In vitro anti-proliferative activity of Argemone gracilenta and identification of some active components. BMC Complement Alter Med 2015;15(13):532-8.
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How to Cite
D, P., S. Mm, and R. A. “ANTICANCER ACTIVITY OF ARGEMONE MEXICANA L (FLOWERS) AGAINST HUMAN LIVER CANCER (HEPG2) CELL LINE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 10, Sept. 2017, pp. 351-3, doi:10.22159/ajpcr.2017.v10i10.20744.
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