• PRABHAKARAN D Department of Chemistry, Periyar E.V.R. College (Autonomous), Trichy, Tamil Nadu, India.
  • RAJESHKANNA A Department of Chemistry, Periyar E.V.R. College (Autonomous), Trichy, Tamil Nadu, India.
  • SENTHAMILSELVI MM Department of Principal, Kamarajar Government Arts College, Surandai, Tamil Nadu, India.


Objective: The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of the solid powder obtained from the ethyl acetate fraction from the flower Opuntia stricta.

Methods: The flower extract was evaluated for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and reducing power assay was carried out by ferric-reducing capacity (FRC) assay method. The in vitro anti-inflammatory activity was evaluated using human peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) to evaluate nitric oxide (NO) production method.

Results: The solid powder obtained from the ethyl acetate fraction from the flower O. stricta showed a good antioxidant activity in scavenging DPPH radical and FRC assay with compared standard sample. This solid powder also showed good anti-inflammatory activity in cell viability (LPS-induced PBMCs) assay and NO assay.

Conclusion: These results suggest that the solid powder obtained from the ethyl acetate fraction from the flower O. stricta has significant antioxidant and anti-inflammatory activities.

Keywords: Antioxidant, Peripheral blood mononuclear cells, Lipopolysaccharide, Opuntia stricta, Ferric-reducing capacity.


1. Balandrin MJ, Klocke JA. Medicinal, aromatic and industrial materials from plants: Medicinal and aromatic plant I. In: Bajaj YP, editor. Biotechnology in Agriculture and Forestry. Vol. 4. New York: Plenum Press; 1988. p. 3-36.
2. Kaur S, Mondal P. Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. J Microbiol Exp 2014;1:1-6.
3. Rao NV. Evaluation of antioxidant potential and qualitative analysis of major polyphenols by RP-HPLC in Nymphaea nouchali Burm. Int J Pharm Pharm Sci 2010;2:98-104.
4. Narayanaswamy N, Balakrishnan KP. Evaluation of some medicinal plants for their antioxidant properties. Int J PharmTech Res 2011;3:381-5.
5. Gülçin I. Comparison of in vitro antioxidant and antiradical activities of L-tyrosine and L-Dopa. Amino Acids 2007;32:431-8.
6. Halliwell B. How to characterize an antioxidant: An update. Biochem Soc Symp 1995;61:73-101.
7. Ramassamy C. Emerging role of polyphenolic compounds in the treatment of neurodegenerative diseases: A review of their intracellular targets. Eur J Pharmacol 2006;545:51-64.
8. Jayaseelan M, Arumugam T, Thangaraj N. Evaluation of antioxidant and anti-inflammatory activities of corallocarpus epigaeus rhizomes. Int J Pharm Biomed Res 2014;5:18-24.
9. Pourmorad F, Hosseinimehr SJ. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5:66-76.
10. Brodsky M, Halpert G, Albeck M, Sredni B. The anti-inflammatory effects of the tellurium redox modulating compound, AS101, are associated with regulation of NFkappaB signaling pathway and nitric oxide induction in macrophages. J Inflamm (Lond) 2010;7:3.
11. Margret RJ, Kumaresan S, Ravikumar S. A preliminary study on the anti-inflammatory activity of methanol extract of Ulva lactuca in rat. J Environ Biol 2009;30:899-902.
12. Chandrika M, Chellaram M. Efficacy of antioxidation and anti- inflammation of the leaf extracts of Borreria hispida. Int J Pharm Pharm Sci 2016;8:369-72.
13. Merina AJ, Kesavan D, Sulochana D. Isolation and antihyperglycemic activity of flavonoid from flower petals of Opuntia stricta. Pharm Chem J 2011;45:317-21.
14. Lim TK. Edible Medicinal and Non-Medicinal Plants. Vol. 1. 2012. p. 687-92.
15. Castellar MR, Obón JM, Alacid M, Fernández-López JA. Fermentation of Opuntia stricta (Haw.) fruits for betalains concentration. J Agric Food Chem 2008;56:4253-7.
16. Ahmed MS, El Tanbouly ND, Islam WT, Sleem AA, El Senousy AS. Antiinflammatory flavonoids from Opuntia dillenii (Ker-gawl) haw. Flowers growing in Egypt. Phytother Res 2005;19:807-9.
17. Huang X, Li Q, Li H, Guo L. Neuroprotective and antioxidative effect of cactus polysaccharides in vivo and in vitro. Cell Mol Neurobiol 2009;29:1211-21.
18. Gupta RS, Sharma R, Sharma A, Chaudhudery R, Bhatnager AK, Dobhal MP, et al. Antispermatogenic effect and chemical investigation of Opuntia dillenii. Pharm Biol 2002;40:411-5.
19. Saleem R, Ahmad M, Azmat A, Ahmad SI, Faizi Z, Abidi L, et al. Hypotensive activity, toxicology and histopathology of opuntioside-I and methanolic extract of Opuntia dillenii. Biol Pharm Bull 2005;28:1844-51.
20. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-200.
21. Adedapo AA, Jimoh FO, Koduru S, Afolayan AJ, Masika PJ. Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea. BMC Complement Altern Med 2008;8:53.
22. Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharm Sci 2010;2:146-56.
23. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem 1996;239:70-6.
24. Kumar DR, Pooja M, Harika K, Haswitha E, Nagabhushanamma G, Vidyavathi N. In-vitro antioxidant activities, total phenolics and flavonoid contents of whole plant of Hemidesmus indicus Linn. Asian J Pharm Clin Res 2013;6:249-51.
25. Lim CS, Lim SL. Ferric reducing capacity versus ferric reducing antioxidant power for measuring total antioxidant capacity. Lab Med 2013;44:51-5.
26. Jenny M, Klieber M, Zaknun D, Schroecksnadel S, Kurz K, Ledochowski M, et al. In vitro testing for anti-inflammatory properties of compounds employing peripheral blood mononuclear cells freshly isolated from healthy donors. Inflamm Res 2011;60:127-35.
27. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR, et al. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem 1982;126:131-8.
28. Saravanan S, Islam VI, Thirugnanasambantham K, Pazhanivel N, Raghuraman N, Paulraj MG, et al. Swertiamarin ameliorates inflammation and osteoclastogenesis intermediates in IL-1? induced rat fibroblast-like synoviocytes. Inflamm Res 2014;63:451-62.
29. Ignácio SR, Ferreira JL, Almeida MB, Kubelka CF. Nitric oxide production by murine peritoneal macrophages in vitro and in vivo treated with Phyllanthus tenellus extracts. J Ethnopharmacol 2001;74:181-7.
30. Richaupadhyay U, Chaurasia JK, Tiwari KN, Singh K. Antioxidant property of aerial parts and root of Phyllanthus fraternus webster, an important medicinal plant. Sci World J 2014;2014:2-5.
31. Sumanya H, Lavanya R, Reddy RU. Evaluation of in vitro anti-oxidant and anti-arthritic activity of methanolic extract of marine green algae Caulerpa racemosa. Int J Pharm Pharm Sci 2015;7:340-3.
32. Saha MR, Hasan SM, Akter R, Hossain MM, Alam MS, Alam MA, et al. In vitro free radical scavenging activity of methanol extract of the leaves of Mimuso pselengi Linn. Bangl J Vet Med 2008;6:197-202.
33. Förstermann U, Kleinert H. Nitric oxide synthase: Expression and expressional control of the three isoforms. Naunyn Schmiedebergs Arch Pharmacol 1995;352:351-64.
9 Views | 19 Downloads
How to Cite
D, P., R. A, and S. MM. “IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF THE FLOWER EXTRACTS OF OPUNTIA STRICTA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 208-12,
Original Article(s)