• Dipali M Atole Department of Pharmaceutics, Government College of Pharmacy, Aurangabad, Maharashtra, India
  • Hrishikesh H Rajput Department of Pharmaceutics, Government College of Pharmacy, Aurangabad, Maharashtra, India


 Rapid and easy analytical methods are needed due to increasing number of multicomponent formulations, biotherapeutic products and samples of complex matrix in que. Number of Ultraviolet (UV) spectrophotometric methods used for these purpose. Different types of UV spectrometric methods developed on the basis of principle of additivity, absorbance difference, processing absorption spectra. The aim of this review is to present information on simultaneous equation method, difference spectrophotometry, derivative spectrophotometry, absorbance ratio spectra, derivative ratio spectra, successive ratio - derivative spectra, Q-absorbance ratio method, absorptivity factor method, dual wavelength method, absorption factor method, multivariate chemometric methods, and isosbestic point method. A brief summary on theories, mathematical background and some applications of these methods are presented here.

Keywords: Ultraviolet spectroscopy, Simultaneous equation method, Derivative spectrophotometry, Derivative ratio spectra, Isosbestic point method, Multivariate chemometric methods.


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How to Cite
Atole, D., and H. Rajput. “ULTRAVIOLET SPECTROSCOPY AND ITS PHARMACEUTICAL APPLICATIONS- A BRIEF REVIEW”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 2, Feb. 2018, pp. 59-66, doi:10.22159/ajpcr.2018.v11i2.21361.
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