ANTIOXIDANT ACTIVITY OF RED DRAGON FRUIT PEEL (HYLOCEREUS POLYRHIZUS (F.A.C. WEBER) BRITTON AND ROSE) ISOLATES USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL METHOD


Sri Wahdaningsih, Subagus Wahyuono, Sugeng Riyanto, Retno Murwanti

Abstract


 

 Objective: Indonesia is a country which has various natural resources including medicinal plants. Among the plants, red dragon fruit is interesting to be studied. In this study, antioxidant activity of red dragon fruit peel (Hylocereus polyrhizus (F.A.C. Weber) Britton and Rose) isolate was tested using 2,2-diphenyl-1-picrylhydrazyl method.

Methods: Isolation of active compounds was performed through the application of vacuum liquid chromatography and preparative thin-layer chromatography (TLC). Antioxidant activity was tested using both TLC and spectrophotometry.

Result: Results revealed that the values of IC50 of isolate 1 and 2 were 2.952, 14 μg/mL and 25.635,95 μg/mL, respectively.

Conclusion: Isolate of dragon fruit has antioxidant activity. The compound which results in antioxidant activity is terpenoid and steroid.


Keywords


Red dragon fruit peel, Isolate, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl.

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About this article

Title

ANTIOXIDANT ACTIVITY OF RED DRAGON FRUIT PEEL (HYLOCEREUS POLYRHIZUS (F.A.C. WEBER) BRITTON AND ROSE) ISOLATES USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL METHOD

Keywords

Red dragon fruit peel, Isolate, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl.

DOI

10.22159/ajpcr.2018.v11i1.21519

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 1 January 2018 Page: 124-128

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Sri Wahdaningsih
Departement of Pharmacy, Faculty of Medicine, Tanjungpura University, Pontianak, Indonesian
Indonesia

Subagus Wahyuono
Faculty of Pharmacy, Gadjah Mada University, Indonesia
Indonesia

Sugeng Riyanto
Faculty of Pharmacy, Gadjah Mada University, Indonesia
Indonesia

Retno Murwanti
Faculty of Pharmacy, Gadjah Mada University, Indonesia
India


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