SEROVAR PROFILE AND DETECTION OF INVA VIRULENCE GENE AMONG NON-TYPHOIDAL SALMONELLAE SEROVARS ISOLATED FROM ACUTE GASTROENTERITIS CASES IN COASTAL KARNATAKA, SOUTHERN INDIA


Sohan Bangera, Shashikiran Umakanth, Ram Bhat, Asha Kamath, Mamatha Ballal, Asish Kumar Mukhopadhyay

Abstract


 

 Objective: Non-typhoidal salmonellosis is one of the leading zoonosis in the world caused by non-typhoidal Salmonella (NTS). Invasive infections with NTS serovars occurs due to the presence of virulence genes like invA along with the immunosuppressive conditions of the patient. The study was conducted to isolate and identify the NTS serovars and their antimicrobial resistance profile from patients with diarrhea and also to detect the virulence marker – invA gene among these NTS serovars.

Methods: A prospective cross-sectional study was conducted from January 2015 to December 2016 at the Enteric Diseases Division, Kasturba Medical College, Manipal. 1218 fecal specimens were collected from patients with diarrhea and before antibiotic treatment. NTS serovars were identified, serotyped and then screened for the presence of invA virulence gene.

Results: A total of 33 (2.7%) NTS was isolated. Salmonella typhimurium (33.34%) was predominant followed by Salmonella oslo (30.3%). Out of 33 NTS, invA was positive for 28 isolates (84.8%) of which 25 (89.3%) patients were febrile which was statistically significant (p=0.000).

Conclusion: Non-typhoidal salmonellosis is an emerging global infection among immunocompromised patients. Our study showed an association between the invA gene and febrile illness among the patients suffering. Thus, this study highlights the importance of invA as a significant marker for bloodstream invasion.


Keywords


Non-typhoidal salmonellosis, invA gene, Virulence, Non-typhoidal Salmonella, Bloodstream infection.

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About this article

Title

SEROVAR PROFILE AND DETECTION OF INVA VIRULENCE GENE AMONG NON-TYPHOIDAL SALMONELLAE SEROVARS ISOLATED FROM ACUTE GASTROENTERITIS CASES IN COASTAL KARNATAKA, SOUTHERN INDIA

Topics

health sciences

Keywords

Non-typhoidal salmonellosis, invA gene, Virulence, Non-typhoidal Salmonella, Bloodstream infection.

DOI

10.22159/ajpcr.2018.v11i1.21839

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 1 January 2018 Page: 162-166

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Sohan Bangera
MSc, Ph.D Research Scholar, Enteric Diseases Division, Department of Microbiology, Kasturba Medical College, Manipal University, Manipal
India

Shashikiran Umakanth
‎Professor of Medicine, Melaka Manipal Medical College, Manipal University Unit Head-Medicine, Dr. TMA Pai Hospital, Udupi, Manipal University
India

Ram Bhat
Professor of Medicine, Kasturba Medical College, Manipal, Manipal University
India

Asha Kamath
Associate Professor of Community Medicine, Kasturba Medical College, Manipal Manipal University
India

Mamatha Ballal
Address Professor of Microbiology Enteric Diseases Division – Incharge Central Research Lab Kasturba Medical College, Manipal University, Manipal-576104,
India

Asish Kumar Mukhopadhyay
Scientist E, Bacteriology Division, National Institute of Cholera and Enteric Diseases (NICED), Kolkata, India.
India


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