ANTIBIOGRAM PROFILING OF HELICOBACTER PYLORI STRAINS AND THE EFFICACY OF BRASSICA CAPITATA AGAINST RESISTANT STRAINS ISOLATED FROM THE PATIENTS SUFFERING FROM GASTRODUODENAL DISEASES IN GUWAHATI, ASSAM


Shweta Mahant, Valentina Gehlot, Seema Bhatnagar, Megha Rikhi, Asish Kumar Mukhopadhyay, Sangitanjan Dutta, Anil Agarwal, Kunal Das, Rajashree Das

Abstract


 

 Objective: Helicobacter pylori resistance toward commonly used antibiotics is increasing leading to the treatment failure; hence, our aim is to determine the antibiogram susceptibility pattern of H. pylori strains isolated from Guwahati, Assam (Northeast India) and also to test the efficacy of the Brassica capitata against the multi and dual drug-resistant strains of North and Northeast India.

Methods: Minimum inhibitory concentration of different antibiotics was determined by agar dilution method. Disc diffusion method was used to check the efficacy of B. capitata against clarithromycin (CLR), metronidazole (MTZ), and levofloxacin (LEV)-resistant H. pylori strains.

Results: All the H. pylori strains were 100% sensitive to CLR, tetracycline, amoxicillin, and furazolidone. 72.8% of the strains were sensitive toward MTZ and 54.5% were sensitive toward LEV. B. capitata showed good efficacy against the resistant strains of H. pylori of North and Northeast India.

Conclusion: Most of the H. pylori strains from Northeast India were sensitive toward the commonly used antibiotics for the treatment regime. B. capitata is effective against H. pylori infection, suggesting its potential as an alternative therapy, and opens the way for further studies on identification of novel antimicrobial targets of B. capitata.


Keywords


Helicobacter pylori, Antibiotic Resistance, Brassica capitata.

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About this article

Title

ANTIBIOGRAM PROFILING OF HELICOBACTER PYLORI STRAINS AND THE EFFICACY OF BRASSICA CAPITATA AGAINST RESISTANT STRAINS ISOLATED FROM THE PATIENTS SUFFERING FROM GASTRODUODENAL DISEASES IN GUWAHATI, ASSAM

Topics

Bacteriology

Keywords

Helicobacter pylori, Antibiotic Resistance, Brassica capitata.

DOI

10.22159/ajpcr.2018.v11i8.25220

Date

07-08-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 8 August 2018 Page: 176-180

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Shweta Mahant
Amity Institute of Biotechnology, Amity University, Noida – 201 303, Uttar Pradesh, India.
India

Valentina Gehlot
Amity Institute of Biotechnology, Amity University, Noida – 201 303, Uttar Pradesh, India.
India

Seema Bhatnagar
Amity Institute of Biotechnology, Amity University, Noida – 201 303, Uttar Pradesh, India.
India

Megha Rikhi
Amity Institute of Biotechnology, Amity University, Noida – 201 303, Uttar Pradesh, India.
India

Asish Kumar Mukhopadhyay
Department of Bacteriology, National Institutes of Cholera and Enteric Disease, Kolkata, West Bengal, India
India

Sangitanjan Dutta
Department of Gastroenterology, Gauhati Medical Collages, Guwahati - 781 024, Assam, India.
India

Anil Agarwal
Department of Gastroenterology, Guwahati Medical Collages,Guwahati - 781 024, Assam, India.
India

Kunal Das
Max Super Specialty Hospital, Vaishali, Ghaziabad. Uttar Pradesh, India.
India

Rajashree Das
Amity Institute of Biotechnology, Amity University, Noida – 201 303, Uttar Pradesh, India.
India


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