EVALUATION OF ANTIRADICAL SCAVENGER ACTIVITY OF EXTRACT AND COMPOUNDS FROM ETLINGERA CALOPHRYS STEMS


Sahidin I, Wahyuni Wahyuni, Muh Hajrul Malaka, Jabbar A, Imran Imran, Marianti A Manggau

Abstract


 Objective: This study was aimed to isolate and identify the chemical compounds from the methanol extract of the stems of Etlingera calophrys as well as to evaluate their potential as free radical scavengers.

Methods: The isolated compounds were obtained using vacuum liquid chromatography (VLC) and radial chromatography techniques. The structures of these compounds were determined on the basis of their measured spectroscopic evidence (infrared, one dimentional (1D), and 2D nuclear magnetic resonance) and by comparison with literature data. The free radical scavenging activity of the methanol extract of the stems and the isolated compounds were measured using qualitative and quantitative 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays.

Results: Yakuchinone A (1), p-hydroxybenzoic acid (2), and stigmasterol (3) were successfully isolated and identified from the stems of E. calophyrs. The methanol extract and compounds 1–3 showed free radical scavenging activity with half maximal inhibitory concentration (IC50) values of 38.36, 39.07, 46.60, and 153.4 μg/mL, respectively, as compared to ascorbic acid with an IC50 value of 10.53 μg/mL.

Conclusions: Yakuchinone A (1) and p-hydroxybenzoic acid (2) are first reported from the genus Etlingera. The presence of these compounds may contribute to the free radical scavenging activity of the methanol extract of the stems of E. calophrys. This finding suggested that E. calophrys may serve as a source of natural antioxidant compounds and could be used in the development of antioxidant herbal preparation.


Keywords


Etlingera calophrys, Zingiberaceae, Stem extract, Compounds, 2,2-diphenyl-1-picrylhydrazyl.

| PDF |

References


Sahidin, Hakim EH, Juliawaty LD, Syah YM, bin Din L, Ghisalberti EL, et al. Cytotoxic properties of oligostilbenoids from the tree barks of Hopea dryobalanoides. Z Naturforsch C 2005;60:723-7.

Juliawaty LD, Sahidin, Hakim EH, Achmad SA, Syah YM, Latip J, et al. A 2-arylbenzofuran derivative from Hopea mengarawan. Nat Prod Commun 2009;4:947-50.

Muhammad N, Din LB, Sahidin I, Hashim SF, Ibrahim N, Zakaria Z, et al. Acuminatol and other antioxidative resveratrol oligomers from the stem bark of Shorea acuminata. Molecules 2012;17:9043-55.

Sahidin I, Wahyuni W, Malaka MH, Imran I. Antibacterial and cytotoxic potencies of stilbene oligomers from stem barks of Baoti (Dryobalanops lanceolata) growing in Kendari-Indonesia. Asian J Pharm Clin Res 2017;10:139-43.

Sahidin I, Ardiansyah S, Taher M, Manggau MA. Terpenoids from the stem bark of Jatropha plants and their biological activities. Makara Sains 2011;15:106-10.

Sahidin I, Yamin N, Ginting S, Manggau MA, Lukman L. Cytotoxic potency of diterpenes from Jatropha plants. Int J Pharm Pharm Sci 2013;5:3-6.

Sabandar CW, Ahmat N, Jaafar FM, Sahidin I. Medicinal property, phytochemistry and pharmacology of several Jatropha species (Euphorbiaceae): A review. Phytochemistry 2013;85:7-29.

Rosandy AR, Din LB, Yaacob WA, Yusoff NI, Sahidin I, Latip J, et al. Isolation and characterization of compounds from the stem bark of Uvaria rufa (Annonaceae). Malays J Anal Sci 2013;17:50-8.

Al Muqarrabun LM, Ahmat N, Ruzaina SA, Ismail NH, Sahidin I. Medicinal uses, phytochemistry and pharmacology of Pongamia pinnata (L.) Pierre: A review. J Ethnopharmacol 2013;150:395-420.

Ruslin M, Asmawi MZ, Rianse U, Sahidin I, Dhianawaty D, Soemardji AA, et al. Anti-hypertensive activity of alang-alang (Imperata cylindrica L.) Beauv. Root methanolic extract on male Wistar rat. Int J Res Pharm Sci 2013;4:537-42.

Sahidin I, Nohong N, Sani A, Manggau MA, Sukohar A, Widodo H, et al. Radical scavenging activity of triterpene steroids from stem of Polygonum pulchrum Bl. Int J Pharm Pharm Sci 2014;6:350-4.

Sahidin I, Suwandi A, Nohong N, Manggau MA. Profile of anticancer and radical scavenging activities of steroidsfrom stems of Polygonum pulchrum. Int J Pharm Sci Res 2015;6:2178-84.

Jalil J, Sabandar CW, Ahmat N, Jamal JA, Jantan I, Aladdin NA, et al. Inhibitory effect of triterpenoids from Dillenia serrata (Dilleniaceae) on prostaglandin E2 production and quantitative HPLC analysis of its koetjapic acid and betulinic acid contents. Molecules 2015;20:3206-20.

Hartati R, Suganda AG, Fidriyanni I, Ginting TM. Total flavonoid content and antimicrobial properties of four species of Zingiberaceae. Int J Pharm Pharm Sci 2014;6:142-4.

Poulsen AD. Etlingera of Sulawesi. Sabah: Natural History Publications; 2006.

Chan EW, Lim YY, Ling SK, Tan SP, Lim KK, Khoo MG. Caffeoylquinic acids from leaves of Etlingera species (Zingiberaceae). LWT Food Sci Technol 2009a;42:1026-30.

Williams CA, Harborne JB. The leaf flavonoids of Zingiberales. Biochem Syst Ecol 1997;5:221-9.

Mahdavi B. Chemical constituents of aerial parts of Etlingera brevilabrum (Zingiberaceae). Der Pharm Chem 2014;6:360-5.

Yahya MA, Yacob WA, Nazlina I. Isolation of chemical constituents from rhizomes of Etlingera sphaerocephala Var. Grandiflora. Malays J Anal Sci 2011;15:22-6.

Ficker CE, Smith ML, Susiarti S, Leaman DJ, Irawati C, Arnason JT. Inhibition of human pathogenic fungi by members of Zingiberaceae used by the Kenyah (Indonesian Borneo). J Ethnopharmacol 2003;85:289-93.

Chan EW, Lim YY, Wong SK, Lianto FS, Wong SK, Lim KK, et al. Antioxidant and tyrosinase inhibition properties of leaves and rhizomes of Zinger species. Food Chem 2008;109:477-83.

Lachumy SJ, Sasidharan S, Sumathy V, Zuraini Z. Pharmacological activity, phytochemical analysis, and toxicity of methanol extract of Etlingera elatior (torch ginger) flowers. Asian Pac J Trop Med 2010;3:769-74.

Wijekoon MM, Bhat R, Karim AA. Effect of extraction solvents on the phenolic compounds and antioxidant activities of bungakantan (Etlingera elatior Jack) infloroscence. J Food Comp Anal 2011;24:615-9.

Chan EW, Lim YY, Wong SK, Lim KK, Tan SP, Lianto FS, et al. Effects of drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chem 2009b;113:166-72.

Chan EW, Lim YY, Omar M. Antioxidant and antibacterial activity of leaves of Etlingera species (Zingiberaceae) in Peninsular Malaysia. Food Chem 2007;104:1586-93.

Chiang EC, Yan LY, Ali NA. Composition and antibacterial activity of essential oils from leaves of Etlingera species (Zingiberaceae). Int J Ambient Syst Appl 2010;1:1-12.

Sudhakaran A, Radha RK. Evaluation of accute and dermal toxicity of essential oil of Etlingera fenzlii (Kurz) K. Schaum: An in vivo study. Int J Pharm Pharm Sci Int J 2016;8:69-72.

Chacha M, Bojase-Moleta G, Majinda RR. Antimicrobial and radical scavenging flavonoids from the stem wood of Erythrina latissima. Phytochemistry 2005;66:99-104.

Fidrianni I, Utari P, Ruslan KW. Evaluation of antioxidant capacities, flavonoid, phenolic, carotenoid content from various extracts of four kinds brassica herbs. Int J Pharm Pharm Sci 2014;6:268-72.

Lin RJ, Yen CM, Chou TH, Chiang FY, Wang GH, Tseng YP, et al. Antioxidant, anti-adipocyte differentiation, antitumor activity and anthelmintic activities against Anisakis simplex and Hymenolepsis nana of yakuchinone a from Alpinia oxyphylla. BMC Complement Altern Med 2013;13:1-13.

Riaz T, Abbasi MA, Aziz-Ur-Rehman AU, Ajaib M. Isolation, structure elucidation and antioxidant screening of some natural products from Colebrookia oppositifolia. Biosci Res 2012;9:68-76.

Goss JA. Physiology of Plants and Their Cells. New York: Pergamon Press Inc.; 1973. p. 286.

Turkoglu A, Kivrak I, Mercan N, Duru ME, Gezer K, Turkoglu H. Antioxidant and antimicrobial activities of Morcella conica Pers. Afr J Biotechnol 2006;5:1146-50.




About this article

Title

EVALUATION OF ANTIRADICAL SCAVENGER ACTIVITY OF EXTRACT AND COMPOUNDS FROM ETLINGERA CALOPHRYS STEMS

Topics

Phytochemistry

Keywords

Etlingera calophrys, Zingiberaceae, Stem extract, Compounds, 2,2-diphenyl-1-picrylhydrazyl.

DOI

10.22159/ajpcr.2018.v11i2.22535

Date

01-02-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 2 February 2018 Page: 238-241

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Sahidin I
Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari 93232, Indonesia.
Indonesia

Wahyuni Wahyuni
Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari 93232, Indonesia.
Indonesia

Muh Hajrul Malaka
Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari 93232, Indonesia.
Indonesia

Jabbar A
Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Kendari 93232, Indonesia.
Indonesia

Imran Imran
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Halu Oleo, Kendari 93232, Indonesia.
Indonesia

Marianti A Manggau
Department of Pharmacy, Faculty of Pharmacy, Universitas Hasanuddin, Makassar 90245, Indonesia.
Indonesia


Article Tools


Email this article (Login required)
Email the author (Login required)

Refbacks

  • There are currently no refbacks.