EVALUATION OF AQUEOUS FRUIT EXTRACT OF TAMARINDUS INDICA (L) FOR INHIBITION OF TUMOR NECROSIS FACTOR-a AND CYCLO OXYGENASE ENZYMES IN EXPERIMENTAL ANIMAL

  • Mohammad Daud Ali Department of Pharmacology, Adarsh Vijendra Institute of Pharmaceutical Sciences, Shobhit University, Gangoh, Saharanpur - 247 341, Uttar Pradesh, India.
  • Atul Kumar Gupta Department of Pharmacology, Adarsh Vijendra Institute of Pharmaceutical Sciences, Shobhit University, Gangoh, Saharanpur - 247 341, Uttar Pradesh, India.
  • Arif Naseer Department of Pharmacology, Adarsh Vijendra Institute of Pharmaceutical Sciences, Shobhit University, Gangoh, Saharanpur - 247 341, Uttar Pradesh, India.
  • Mohd Aamir Mirza Department of Product Development, New Zealand Fulvic Limited, Mount Maunganui, Tauranga 3116, New Zealand.
  • Sabir Afzal Department of Pharmacology, Adarsh Vijendra Institute of Pharmaceutical Sciences, Shobhit University, Gangoh, Saharanpur - 247 341, Uttar Pradesh, India.

Abstract

 Objective: The main objective of this study was to assess tumor necrosis factor-α (TNF)-α and cyclooxygenase enzymes (COX) inhibition potency of Tamarindus indica Linn. in comparison to standard drug (indomethacin).

Methods: Three plants are selected for the studies, namely: Aloe vera (L.), Terminalia chebula Reitz., and T. indica Linn. Estimation of TNF-α in serum (at 1:10 dilution in PBS) was performed using the immunoenzymatic (ELISA) technique. COX inhibitor screening assay kits were used for estimation of COX.

Result: All three plant extracts showed a potent significant inhibition of the COX enzyme as compared to the positive control and standard drug when the animal was administered with 400 mg/kg. These studies indicate that the T. indica plant extract showed significant COX inhibition even at low dose. All the extracts were effective anti-inflammatory in nature, however, T. indica extracts at a dose of 400 mg/kg were found to be most potent. It was found to be comparable with that of Indomethacin 10 mg/kg body weight.

Conclusion: The anti-inflammatory activity expressed by all the three plants A. vera (L.), T. chebula Reitz., and T. indica L. Among all three plants T. indica (L) was found to be more active against both TNF-α and COX, and it was comparable to standard drug Indomethacin. Need further studies to elucidate the exact secondary metabolite by which these plants express this activity.

Keywords: Inflammation, Tamarindus indica (L), Tumor necrosis factor-α, Cyclooxygenase enzymes.

Author Biography

Mohammad Daud Ali, Department of Pharmacology, Adarsh Vijendra Institute of Pharmaceutical Sciences, Shobhit University, Gangoh, Saharanpur - 247 341, Uttar Pradesh, India.
Mohammad Daud Ali M. Pharma (Pharmacy practice) and PhD Scholar (Pharmacy)School of Pharmacy –Adarsh Vijendra Institute of Pharmaceutical Sciences, Shobhit University, Gangoh, Dist. Saharanpur (U.P), 247341, India.

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Ali, M. D., A. K. Gupta, A. Naseer, M. A. Mirza, and S. Afzal. “EVALUATION OF AQUEOUS FRUIT EXTRACT OF TAMARINDUS INDICA (L) FOR INHIBITION OF TUMOR NECROSIS FACTOR-a AND CYCLO OXYGENASE ENZYMES IN EXPERIMENTAL ANIMAL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 3, Mar. 2018, pp. 57-61, doi:10.22159/ajpcr.2018.v11i3.23027.
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