ANTIBACTERIAL EFFECT OF VIRGIN COCONUT OIL ON (ACTINOMYCES SP.) THAT CAUSES DENTAL BLACK STAIN IN CHILDREN

Authors

  • Priscilla Lavine Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • Eva Fauziah Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • Mochamad Fahlevi Rizal Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia
  • Sarworini Bagio Budiardjo Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i2.23199

Keywords:

Actinomyces sp, Dental black stain, Virgin coconut oil

Abstract

 Objective: This study aimed to assess the effectiveness of virgin coconut oil (VCO) as a natural antibacterial agent to reduce the viability of the chromogenic bacteria Actinomyces sp., which causes dental black stains.

Methods: Actinomyces sp. was isolated from the saliva of a child diagnosed with black stain. Each streak of bacteria was cultured on a selective medium Actinomyces agar and confirmed visually and through a gram staining procedure. Each bacterial culture was exposed to VCO in concentrations of 12.5%, 25%, 50%, and 100%. Afterward, viability testing with a methyl-thiazolyl-tetrazolium assay was conducted, and the results were read using an enzyme-linked immunosorbent assay reader.

Results: The reduction of bacterial viability of Actinomyces sp. showed a significant difference between the negative control group and the groups treated with various concentrations of VCO 12.5%, 25%, 50%, and 100%.

Conclusion: The minimum concentration of VCO necessary to kill Actinomyces sp. is VCO 12.5%.

Author Biographies

Priscilla Lavine, Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Department of Pediatric Faculty of Dentistry

Eva Fauziah, Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Department of Pediatric Faculty of Dentistry

Mochamad Fahlevi Rizal, Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Department of Pediatric Faculty of Dentistry

Sarworini Bagio Budiardjo, Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Department of Pediatric Faculty of Dentistry

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Published

2018-02-01

How to Cite

Lavine, P., E. Fauziah, M. F. Rizal, and S. B. Budiardjo. “ANTIBACTERIAL EFFECT OF VIRGIN COCONUT OIL ON (ACTINOMYCES SP.) THAT CAUSES DENTAL BLACK STAIN IN CHILDREN”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 2, Feb. 2018, pp. 333-5, doi:10.22159/ajpcr.2018.v11i2.23199.

Issue

Section

Original Article(s)