ANTI-INFLAMMATORY ACTIVITY OF TINOCRISPOSIDE BY INHIBITING NITRIC OXIDE PRODUCTION IN LIPOPOLYSACCHARIDES-STIMULATED RAW 264.7 CELLS


Adek Zamrud Adnan, Muhammad Taher, Tika Afriani, Annisa Fauzana, Dewi Imelda Roesma, Andani Eka Putra

Abstract


 Objective: The aim of this study was to investigate in vitro anti-inflammatory activity of tinocrisposide using lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophage cells. Tinocrisposide is a furano diterpene glycoside that was isolated in our previous study from Tinospora crispa.

Methods: Anti-inflammatory effect was quantified spectrometrically using Griess method by measuring nitric oxide (NO) production after the addition of Griess reagent.

Results: The sample concentrations of 1, 5, 25, 50, and 100 μM and 100 μM of dexamethasone (positive control) have been tested against the LPS-stimulated RAW 264.7 cells, and the results showed NO level production of 39.23, 34.00, 28.9, 20.25, 16.3, and 13.68 μM, respectively, and the inhibition level of 22.67, 33.00, 43.03, 60.10, 68.00, and 73%, respectively.

Conclusions: From the study, it could be concluded that tinocrisposide was able to inhibit the formation of NO in the LPS-stimulated RAW 264.7 cells in concentration activity-dependent manner, with half-maximal inhibition concentration 46.92 μM. It can be developed as anti-inflammatory candidate drug because NO is a reactive nitrogen species which is produced by NO synthase. The production of NO has been established as a mediator in inflammatory diseases.


Keywords


Anti-inflammatory activity, Griess method, Tinocrisposide, Tinospora crispa.

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About this article

Title

ANTI-INFLAMMATORY ACTIVITY OF TINOCRISPOSIDE BY INHIBITING NITRIC OXIDE PRODUCTION IN LIPOPOLYSACCHARIDES-STIMULATED RAW 264.7 CELLS

Topics

Pharmacy, sub discipline Medicinal Chemistry

Keywords

Anti-inflammatory activity, Griess method, Tinocrisposide, Tinospora crispa.

DOI

10.22159/ajpcr.2018.v11i4.23739

Date

01-04-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 4 April 2018 Page: 149-153

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Adek Zamrud Adnan
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Andalas University, Padang, Indonesia.
Indonesia

Muhammad Taher
Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Malaysia.
Indonesia

Tika Afriani
Department of Pharmacy, Mohammad Natsir University, Bukittinggi 26136, Indonesia.
Indonesia

Annisa Fauzana
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Andalas University, Padang, Indonesia.
Indonesia

Dewi Imelda Roesma
Department of Biology, Andalas University, Indonesia.
Indonesia

Andani Eka Putra
Department of Microbiology, Medicinal Faculty, Andalas, University, Indonesia.
Indonesia


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