RELAXATION ACTIVITY OF TETANUS (LEEA AEQUATA L.) LEAF ETHANOLIC EXTRACT ON GUINEA PIG ISOLATED TRACHEA

Authors

  • Nahitma Ginting Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia.
  • Edy Suwarso Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia.
  • Daud Vincen Rumapea Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia.
  • Nerdy Nerdy Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26557

Keywords:

Isolated trachea, Guinea pig, Tetanus leaf, Relaxation

Abstract

 Objective: The aim of this study is to determine the relaxation activity of the ethanolic extract of tetanus leaf (EETL) against contracting isolated guinea pig trachea induced by acetylcholine.

Methods: Isolated trachea was equilibrated for 45 min until a stable condition in the Creb's solution with a temperature of 37°C aerated with carbogen gas (O2:CO2=95%:5%). Test of the relaxation effect conducted after guinea pig trachea contracted with maximum acetylcholine concentration (EC80: 2.0449×10–3 mol) and then a cumulative with concentration of EETL (0.5 mg/mL–4 mg/mL) and concentration of atropine sulfate (6.95×10-6 mg/mL–2.08×10–2 mg/mL).

Results: The results showed that EETL could lower the contraction smooth muscle of guinea pig trachea induced by acetylcholine. EETL at a concentration of 3.5 mg/mL had the ability to relax the muscle. It was not significantly different compared to that of atropine sulfate 6.95×10-3 mg/ mL (p>0.005).

Conclusion: EETL has a relaxation effect on smooth muscle of guinea pig isolated trachea.

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References

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Published

26-04-2018

How to Cite

Ginting, N., E. Suwarso, D. Vincen Rumapea, and N. Nerdy. “RELAXATION ACTIVITY OF TETANUS (LEEA AEQUATA L.) LEAF ETHANOLIC EXTRACT ON GUINEA PIG ISOLATED TRACHEA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 13, Apr. 2018, pp. 24-27, doi:10.22159/ajpcr.2018.v11s1.26557.

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