IN VITRO ANTINEPHROLITHIASIS EFFECT OF BREADFRUIT (ARTOCARPUS ALTILIS (PARK) FOSBERG) LEAVES EXTRACT BY ATOMIC ABSORPTION SPECTROPHOTOMETRY
Keywords:In vitro, Antinephrolithiasis, Artocarpus altilis, Atomic absorption spectrophotometry
Â Objective: The objective of this study is to observe the solubility calcium oxalate as a prototype of kidney stone in breadfruit leaf extract solution (n-hexane extract solution, ethyl acetate extract solution, and ethanol extract solution) by atomic absorption spectrophotometry.
Methods: Research was conducted qualitatively to analyze calcium oxalate solubility in breadfruit leaf extract solution. The solubility of calcium was known by measuring the levels of calcium in extract solution before and after incubation with calcium oxalate. Potassium as a factor that can enhance the solubility of calcium oxalate also measured by atomic absorption spectrophotometry.
Results: The higher concentration of extract solution used in incubation with calciumoxalate, the higher dissolving activity of calcium oxalate. The highest activity was found in ethyl acetate extract to dissolve calcium oxalate. Potassium has a small effect on the activity of dissolving calcium oxalate. Activity may be due to the phytochemical content present in the ethyl acetate extract.
Conclusion: Ethyl acetate extract solution has the highest activity to dissolve calcium compared to n-hexane extract and ethanol extract solution.
Kamal Z, Suparmi, Sumarmi. Identification and Determination of Dissolved Calcium Levels in Cat Whiskers (Orthisiphon aristatus)Water Fraction and Ethyl Acetate Fraction by Atomic Absorption Spectrophotometry. Vol. 1. Proceedings of the Scientific Meeting and Presentation of Basic Research on Nuclear Science and Technology 2013. p. 363-8.
Chikezie PC, Ojiako OA. Herbal medicine: Yesterday, today and tomorrow. Altern Integr Med 2015;4:1-5.
Guinnin FF, Klotoe JR, Ategbo JM. Acute toxicity evaluation of ethanolic extract of Aristolochia albida Duch. leaves on wistar rats liver and kidney functions. Int J Pharm Pharm Res 2017;9:35-40.
Bouhalit S, Kechrid Z, Elfeki A. Effect of silymarin extracted from Silybum marianum on nickel hematotoxicity and nephrotoxicity in male albino wistar rats. Int J Pharm Pharm Res 2017;9:84-9.
Mardiana L. Miracle Leaf Treating Disease. Jakarta: Penebar Swadaya; 2012. p. 28.
Arifin HN, Muchtar H. Efek Diuretik dan Daya Larut Batu Ginjal Dari Ekstrak Etanol Rambut Jagung (Zea mays L.). Prosiding Seminar Nasional Perkembangan Terkini Sains Farmasi dan Klinik III; 2013.
Winston D. Herbal and Nutritional Treatment of Kidney Stones. J Am Herb Guild 2011;10:61-9.
Pearle MS, Calhoun EA, Curhan GC. Uroligic Diseases in America. Washington: US Government Publishing Office; 2004. p. 7.
Gandjar IG, Rohman A. Pharmaceutical Chemistry Analysis. 3rd ed. Yogyakarta: Pustaka Pelajar; 2008. p. 305-19.
Harmita. Method Validation Guidance and the Calculation. Majalah Ilmu Kefarmasian 2004;1:117-30.
Haro GI, Sinaga SM. In vitro test of chive leaves infuse (Allium schoenoprasum, L.) on calcium oxalate solubility using atomic absorption spectrophotometry. Int J Chemtech Res 2016;10:99-102.
Amin B, Feriz HM, Hariri AT, Meybodi NT, Hosseinzadeh H. Protective effects of the aqueous extract of Crocus sativus against ethylene glycol induced nephrolithiasis in rats. EXCLI J 2015;14:411-22.
Hadjzadeh MA, Nama M, Zeynab R, dan Fatemeh BR. Effect of Tyymoquinone on ethylene glycolinduced kidney Calculi in Rats. Urol J 2008;5:149-55.
Saha S, Verma RJ. Bergenia ciliata extract prevents ethylene glycol induced histopathological changes in the kidney. Acta Pol Pharm 2011;68:711-5.
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