ANTIOXIDANT ACTIVITY AND CARDIOPROTECTIVE ACTIVITY OF BANGUN-BANGUN LEAVES (PLECTRANTHUS AMBOINICUS LOUR.) ETHANOLIC EXTRACT
Keywords:Antioxidant, Cardioprotective, Bangun-bangun leaves, Ethanolic extract, Doxorubicin
Objectives: The objectives of this study were to determine the antioxidant activity and cardioprotective activity of bangun-bangun leaves ethanolic extract.
Methods: Bangun-bangun leaves ethanolic extract was obtained by maceration process. The antioxidant activity test was performed by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radical scavenging method with various concentrations of extract. The absorbance was measured by visible spectrophotometric method and calculated the inhibitory concentration (IC50) value for antioxidant activity analysis. Cardioprotective activity test was performed by measuring the cardiac troponin T (cTnT) level, creatine kinase-muscle/brain (CK-MB) level, and histology of the heart tissue. Animals induced with doxorubicin at the 8th day and the 9th day, bangun-bangun leaves ethanolic extract was administered from the 1st day to the 9th day with various doses of extract.
Results: Bangun-bangun leaves ethanolic extract had IC50 value of 57.79 Î¼g/mL. Difference dose of bangun-bangun leaves ethanolic extract shows difference cardioprotective activity. Bangun-bangun leaves ethanolic extract at dose 300 mg/kg bw did not differ significantly to the positive control group and normal group. The higher the dose of an extract the greater the decrease in cTnT and CK-MB levels and increase protection against heart damage.
Conclusion: Bangun-bangun leaves ethanolic extract had strong antioxidant and had cardioprotective activity.Â
Arafa HM, Abdellah MF, Hafez HF. Abatement by naringenin of doxorubicin induced cardiac toxicity in rats. J Egypt Natl Cancer Inst 2005;14:230-91.
Hariana AH. Herbal Medicine and the Activity. Jakarta: Penebar Swadaya; 2005.
Lukhoba CW, Simmonds MSJ, Paton AJ. Plectranthus-a review of ethnobotanical uses. J Ethnopharmacol 2006;103:1-24.
Ministry of Health (Republic of Indoesia). Indonesia Pharmacopoeia. 3rd ed. Jakarta: Department of Health (Republic of Indonesia); 1979.
Chennuru A, Saleem MT. Antioxidant, lipid lowering, and membrane stabilization effect of sesamol against doxorubicininducedcardiomyopathy in experimental rats.BioMed Res Int 2013. DOI: org/10.1155/2013/934239:1-5.
El-Sayed E, Abd Elazeem A, Afify A, Shabana M, Ahmed H. Cardioprotective effects of Curcuma longa L. extracts against doxorubicininduced cardiotoxicity in rats. J Med Plant Res 2011;5:4050.
Adamcova M, Sterba M, Simunek T, Potacova A, Popelova O, Mazurova Y. Troponin as marker of myocardiac damage in druginduced cardiotoxicity. Expert Opin Drug Saf 2005;4:457-65.
Abdel-Raheem I,Taye A, Abouzied M. Cardioprotective effects of nicorandil, a mitochondrial potassium channel opener against doxorubicin induced cardiotoxicity in rats. Basic Clini Pharmacol Toxicol 2013;113:159.
Sudiana I. Teknologi Ilmu Jaringan dan Imunohistokimia. Jakarta: Sagung Seto; 2005.
Amic D, Davidovic-Amic D, Beslo D, Trinajstic N. Structureradical scavenging activity relationships of flavonoids. Croat Chem Acta 2002;76:55.
Krishnamurthy P, Wadhwani A. Antioxidant enzymes and human health. Intech 2012;1:3-9.
Gewirtz D. Critical evaluation of the mechanisms of action proposed for the antitumor effects of the anthracycline antibiotics adriamycin and daunorubicin. Biochem Pharmacol 1999;57:727.
KaiserovÃ¡ H, Simunek T, van der Vijgh WJ, Bast A, KvasnickovÃ¡ E. Flavonoids as protectors against doxorubicin cardiotoxicity-role of iron chelation, antioxidant activity and inhibition of carbonyl reductase. Biochim Biophys Acta 2007;1772:1065-74.
Carlquist M, Frejd T, Gorwa-Grauslund MF. Flavonoids as inhibitors of human carbonyl reductase 1. Chem Biol Interact 2008;174:98-108.
Zheng J, LeeHC, Bin-Sattar MM, Huang Y. Cardioprotective effects of epigallocatechin-3-gallate against doxorubicininduced cardiomyocyte injury. Eur J Pharmacol 2011;652:82-8.
Schimmel KL, Richel DJ, van den Brink RB, Guchelaar HJ. Cardiotoxicity of cytotoxic drugs. Cancer Treat Rev 2004;30:181-91.
Ragavendran P, Sophia D, Arulraj C, Gopalakrishnan VK. Cardioprotective effect of aqueous, ethanol, and aqueous ethanol extract of Aerva lanata (Linn.) against doxorubicin induced cardiomyophaty in rats. Asian Pac J Trop Biomed 2012;2:212-18.
Varadharajan R, Rajalingam D, Palanic S. Mass chromatography mas sepctrometry/mas spectrometry analysis and cardioprotective potential of Cucumis callosus on doxorubicin induced cardiotoxicity in rats. Int J Pharm Pharm Sci 2016;8:239-45.
Ravichandra V, Hanumantharayappa B, Madhava RP. Evaluation of cardioprotective activity of galangin against doxorubicin induced cardiomyopathy. Int J Pharm Pharm Sci 2014;6:86-90.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.