IN VITRO ANTICONVULSANT EFFECT OF ETHYL ACETATE FRACTION OF TITANUS LEAF (LEEA AEQUATA L.) ON ISOLATED COLON
Keywords:Titanus leaf, Colon, Guinea pig, Relaxation, In vitro
Â Objective: The excessive activity of the parasympathetic nervous system in the intestinal smooth muscle is important role in the increased intestinal motility, so antispasmodic medications are needed that can decrease intestinal motility such as atropine. Treatment may also use medicinal plants that are cheaper and easy to obtain, and also believed to have smaller side effects than modern antispasmodic drugs. This study aimed to determine the anticonvulsant or relaxation effects of the ethyl acetate fraction of titanus leaf (EAFTL) against contracted guinea pig colon.
Methods: The parameters measured are smooth muscle relaxation. Before testing, guinea pig colon was equilibrated for 45 min to obtain a stable condition in Tyrode's solution with a temperature of 37Â°C aerated with carbogen gas (O2:CO2) with a ratio of 95%:5%. The relaxing effect of the colon was tested after inducing by acetylcholine chloride; then, each colon was given cumulative concentration of EAFTL and atropine sulfate. The concentration of acetylcholine chloride required to increase the contraction of the guinea pig colon was 1.76Ã—10âˆ’4 Mol. The cumulative concentration of EAFTL given was 0.5â€“4 mg/Ml and cumulative concentration of atropine sulfate given was 6.95Ã—10âˆ’6â€“2.08Ã—10âˆ’2 mg/Ml.
Results: The EAFTL has a relaxing effect. Statistical analysis of EAFTL at a concentration of 4 mg/Ml (100.000Â±1.7417) in reducing the smooth muscle of colon contraction induced by acetylcholine chloride 1.76Ã—10âˆ’4 Mol (p>0.005), has not statistically significant compared to that of atropine sulfate 6.95Ã—10âˆ’3mg/Ml (105.7292Â±0.8161).
Conclusion: EAFTL has relaxing effect on the smooth muscle of the colon.
Farnsworth NR. Biologycal and phytochemical screening of plants. J Pharm Sci 1966;55:263-4. 2. Harborne JB. Phytochemical Methods. Translate by: Kosasih Padmawinata and Iwang Soediro. Phytochemical Methods. Bandung: ITB Press; 1987. p. 147.
Khare CP. Indian Medicinal Plants. New Delhi: Springer Science + Business Media, LCC; 2007. p. 366.
Kitchen I. Textbook of in vitro Practical Pharmacology. London: Blackwell Scientific Publications; 1984. p. 4.
Malinda I. Phytochemical Screening and Antibacterial Activity of Ethanol Extract Titanus Leaf (Leea aequata L.) on Karo Traditional Medicine. Essay. Field. Indonesian: University of North Sumatera; 2015.
Neal MJ. In: Safitri A, editor. Medical Pharmacology at a Glance. 5th ed. Jakarta: Erlangga Press; 2005. p. 20-1.
Nugroho AE. Pharmacology of Essential Medicines in World Health Pharmaceutical Science. Yogyakarta: Students Literature; 2012. p. 16, 22-3, 26-35, 183.
Rahman MA, Imran TB, dan Islam S. Antioxidative, antimicrobial and cytotoxic effects of the phenolics of Leea indica leaf extract. Saudi J Biol Sci 2012;20:222.
Raihan MO, Habib MR, Brishti A, Rahman MM, Saleheen MM, Manna M, et al. Sedative and anxiolytic effects of the methanolic extract of leea indica (Burm. F.) merr. Leaf. Drug Discov Ther 2011;5:185-9.
Robinson T. The Organic Constituents of High Plant. 6th ed. Translate by: Kosasih Padmawinata. Kandungan Organik Tumbuhan Tinggi. 6th Edisi. Bandung: ITB Press; 1995. p. 154-7.
Tiwari T.S. Tetanus. VPD Surveillance Manual. 5th ed. Ch. 161. 2011.
Tyrode MV. The mode of action of some purgative salts. Arch Intern Pharm 1910;17:205-9.
Valenzuela CF, Puglia MP, Zucca S. Update on brain pathologyfocus on Neurotransmitter Systems. Alcohol Res Health 2011;34:106-20.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.