IMMUNOMODULATORY EFFECTS OF ETHANOL EXTRACT ARTEMISIA VULGARIS L. IN MALE RATS

Authors

  • Roma Marbun Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Edy Suwarso Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Yuandani Yuandani Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26619

Keywords:

Immunomodulatory, Artemisia vulgaris, Carbon clearance, Leukocyte

Abstract

 

 Objective: This current study was conducted to investigate the immunomodulatory effects of ethanol extract of Artemisia vulgaris in male rats.

Methods: Immunomodulatory activity was determined by measuring the carbon elimination rate, the total number of leukocytes and differential, as well as antibody titer. The treatment groups were divided into six groups, each group consisting of five male rats. The extract at doses of 50, 100, 200, and 400 mg/kg bw was administered orally for 7 days. The carbon elimination was measured using an ultraviolet-visible spectrophotometer.

Results: The ethanol extract of A. vulgaris at doses of 50, 100, 200, and 400 mg/kg body weight increased the carbon elimination and increased the number of total leukocytes and differential leukocytes, especially neutrophils and neutrophils rod segments as compared to the negative control group (CMC-Na 0.5%) (p<0.05).

Conclusion: The ethanol extract of A. vulgaris possesses immunomodulatory effect.

References

Temraz A, Walid T. Characterization of antioxidant activity of extract from Artemisia vulgaris. Pak J Pharm Sci 2008;21:321-6.

Kresno BS. Imunologi: Diagnosis and laboratory process. Facult Med Publ 2001;5:10-2.

Yuandani, Jantan I, Ilangkovan M, Husain K, Chan KM. Inhibitory effects of compounds from Phyllanthus amarus on nitric oxide production, lymphocyte proliferation, and cytokine release from phagocytes. Drug Des Devel Ther 2016;10:1935-45.

Dash S, Nath LK, Bhise S, Kar PB. Stimulation of immune function activity by the alcoholic root extract of Heracleum nepalense D.Don. Indian J Pharmacol 2006;38:336-40.

Nawab A, Yunus M, Mahdi AA, Gupta S. Evaluation of anticancer properties of medicinal plants from the Indian sub-continent. Mol Cell Pharm J 2011;3:21-9.

Bao Y, Li Y, Jiang Q, Zhao L, Xue T, Hu B, et al. Methylthioadenosine/ S-adenosyl homocysteine nucleosidase (Pfs) of Staphylococcus aureus is essential for the virulence independent of LuxS/AI-2 system. Int J Med Microbiol 2013;303:190-200.

James D, Garcia C, Garg G. Mugwort (Artemisia vulgaris, Artemisia douglasiana, Artemisia argyi) in the treatment of menopause, premenstrual syndrome, dysmenorrhea and attention deficit hyperactivity disorder. Chin Med J 2012;25:116-23.

Shukla SP, Mianty K, Jiran KH. Establishing the reliability of palatal rugae pattern in individual identification (following orthodontic treatment). J Forensic Odontostomatol 2009;29:20-9.

Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci 1966;55:263-4.

Aldi Y, Mahyudin, Handayani D. Activity test of some sub fraction of Herba Meniran ethyl acetate (Phyllanthus niruri Linn.) against active kutan hypersensitivity reactions. J Pharm Sci Tech 2013;18:9-16.

Harborne B. Phytochemical Methods. 3rd ed. United of Kingdom: University of Reading; 2013. p. 214.

Ghosh T, Mitra P, Mitra PK. Effect of leaves of Artemisia vulgaris L. on growth of rats. Int J Herbs Med 2013;1:30.

Faradilla M. Effects of immunomodulator polysaccharide rhizome white ginger (Curcuma zedoaria (Christm.) Roscoe). J Pharm Sci Indones 2014;12:25-7.

Prastiwi R, Tjahyadi, Chusun. Test effect of tonic ethanol extract herb Centella asiatica

Published

2018-04-26

How to Cite

Marbun, R., E. Suwarso, and Y. Yuandani. “IMMUNOMODULATORY EFFECTS OF ETHANOL EXTRACT ARTEMISIA VULGARIS L. IN MALE RATS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 13, Apr. 2018, pp. 245-7, doi:10.22159/ajpcr.2018.v11s1.26619.

Issue

Section

Original Article(s)

Most read articles by the same author(s)

1 2 > >>