RELAXATION ACTIVITY OF TETANUS (LEEA AEQUATA L.) LEAF ETHANOLIC EXTRACT ON GUINEA PIG ISOLATED TRACHEA

Nahitma Ginting; Edy Suwarso; Daud Vincen Rumapea; Nerdy Nerdy

doi:10.22159/ajpcr.2018.v11s1.26557

Authors

Nahitma Ginting Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia.
Edy Suwarso Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia.
Daud Vincen Rumapea Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia.
Nerdy Nerdy Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26557

Keywords:

Isolated trachea, Guinea pig, Tetanus leaf, Relaxation

Abstract

Â Objective: The aim of this study is to determine the relaxation activity of the ethanolic extract of tetanus leaf (EETL) against contracting isolated guinea pig trachea induced by acetylcholine.

Methods: Isolated trachea was equilibrated for 45 min until a stable condition in the Creb's solution with a temperature of 37Â°C aerated with carbogen gas (O2:CO2=95%:5%). Test of the relaxation effect conducted after guinea pig trachea contracted with maximum acetylcholine concentration (EC80: 2.0449Ã—10â€“3 mol) and then a cumulative with concentration of EETL (0.5 mg/mLâ€“4 mg/mL) and concentration of atropine sulfate (6.95Ã—10-6 mg/mLâ€“2.08Ã—10â€“2 mg/mL).

Results: The results showed that EETL could lower the contraction smooth muscle of guinea pig trachea induced by acetylcholine. EETL at a concentration of 3.5 mg/mL had the ability to relax the muscle. It was not significantly different compared to that of atropine sulfate 6.95Ã—10-3 mg/ mL (p>0.005).

Conclusion: EETL has a relaxation effect on smooth muscle of guinea pig isolated trachea.

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References

Atkinson W. Tetanus Epidemiology and Prevention of Vaccine- Preventable Diseases. 12th ed. New York: Public Health Foundation; 2012. p. 291-300.

Khare CP. Indian Medicinal Plants. New Delhi: Springer Science & Business Media, LCC; 2007. p. 366.

Oenema TA. Muscarinic Receptors in Airway Smooth Muscle: Roles in inflammation and remodeling. Dissertation. Netherlands: University of Groningenâ€“Netherlands; 2013. p. 15.

Gosens R, Zaagsma J, Meurs H, Halayko AJ. Muscarinic receptor signaling in the pathophysiology of asthma and COPD. Respir Res 2006;7:1-15.

Hall IP. Second messengers, ion channels and pharmacology of airway smooth muscle. Eur Respir J 2000;4:1120-7.

Soares PM, Pires A, Ana MA, Souza EP, Criddle D. Relaxant effects of the essential oil of Mentha pulegium L. in rat isolated trachea and urinary bladder. J Pharm Pharmacol 2012;64:1777-84.

Saraq B, Altun A, Yildirim S, Yildirim MK, Temiz TK, Bagvican I, et al. Investigation of the relaxant effect mechanism of ketamine on normal and ovalbumin-induced trachea in guinea pigs. Turkiye Klinikleri J Med Sci 2011;31:1145-52.

Carron R, Sanz E, Puebla P, Martin ML, Roman LS, Guerrero MF. Mechanisms of relaxation induced by flavonoid ayanin in isolated aorta rings from wistar rats. Colomb MÃ©d 2010;41:1-7.

Li J, Zhang B. Apigenin protects ovalbumin induced asthma through the regulation of Th17 cells. Fitoterapia 2013;91:298-304.

Raihan MO, Habib MR, Brishti A, Rahman MM, Saleheen MM, Manna M. Sedative and anxiolytic effects of the methanolic extract of Leea indica (Burm. f.) merr. leaf. Drug Discov Ther 2011;5:185-9.

Rahman MA, Imran TB, Islam S. Antioxidative, antimicrobial and cytotoxic effects of the phenolics of Leea indica leaf extract. Saudi J Biol Sci 2012;20:213-25.

Sellers DJ, Williams RC. In: Fryer AD, Christopoulos A, Nathanson NM. Muscarinic agonists and antagonists: Effects on the urinary bladder. Handbook of Experimental Pharmacology: Muscarinic Receptors. Vol. 208. Germany: Springer-Verlag, Berlin Heidelberg; 2012. p. 375- 400.