ANALYSIS ON CALCIUM SOLUBILITY IN KIDNEY STONES (IN VITRO) AND DIURETIC EFFECT (IN VIVO) USING CORN SILK (ZEA MAYS L.) INFUSE

Authors

  • Tuty Roida Pardede Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Muchlisyam Muchlisyam Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26573

Keywords:

Solubility, Kidney stones, Male rats, Corn silk infuse, Diuretic, Atomic absorption spectrophotometry

Abstract

Objective: Investigation has been carried out about infuse of corn silk (Zea mays L.) concerning the solubility of kidney stone in vitro and the diuretic effect in vivo.

Methods: The methodology of research conducted in the form of dissolving kidney stones in corn silk infuse with a concentration of 2%, 4%, 6%, 8%, and 10%, then incubated at 37°C for 4 h. The solubility of calcium is known by measuring the levels of early calcium and after incubated with kidney stones. Potassium (K) is a substance that can dissolve kidney stones in infuse and also measured by atomic absorption spectrophotometry. The diuretic effect study used male rats which were divided into 7 groups that consisted of 1 positive control (treated with furosemide), 1 negative control treated with CMC Na 0.5 % and 5 groups treated with infuse of corn silk in 5 different concentration (2%, 4%, 6%, 8%, and 10%).

Results: Increase in calcium levels after incubation with kidney stone using corn silk infuse with various concentrations. The levels of Ca dissolved in the infuse of 2%, 4%, 6%, 8%, and 10%, respectively, were 2.2600 μg/ml, 5.5733 μg/ml, 7.9267 μg/ml, 10.9233 μg/ml, and 8.7667 μg/ml. It was found that the urine volume for 24 h was significant with 2%, 8%, and 10% corn silk infuse.

Conclusion: Infuse of corn silk could decrease kidney stones, and it was diuretic. This research supported the aim of traditional corn silk decoction for the same intention.

References

Indonesian Health Ministry. National Traditional Medicine Policy. Jakarta, Indonesia: Indonesian Health Ministry; 2007.

Corwin JE. Handbook of Pathophysiology. 3rd ed. Jakarta: EGC; 2009. p. 715.

Cáceres A, Girón LM, Martínez AM. Diuretic activity of plants used for the treatment of urinary ailments in guatemala. J Ethnopharmacol 1987;19:233-45.

Mursito B. Traditional Herb for Treatment of Heart. Jakarta: AQ8 ???; 2002.

Arifin NH, Muchtar H. Proceedings of the National Seminar on Development of Pharmaceutical Science and Clinical Technician; 2013.

Hidayati MA, dan Anggraini HY. The effect of frequency from the using of tea which made from dried tempuyung leaf (Sonchus arvensis) to the solubility of calcium oxalate (CaC2O4). J Kesehatan 2009;2: ???.

???

Indonesian Health Ministry. Indonesian Pharmacopoeia. Jakarta, Indonesia: Indonesian Health Ministry; 1995.

Harmita. Instruction for validating methods. Pharmaceut Sci Mag 2004;1:117-22.

Ermer J, Miller JH. Method Validation in Pharmaceutical Analysis. Weinheim: Wiley-Vch Verlag GmbH & Co. KgaA.; 2005.

Gandjar IG, Rohman A. Pharmaceutical Analysis. Yogyakarta: Pustaka Pelajar; 2008.

Corwin JE. Handbook of Pathophysiology. 3rd ed. Jakarta: EGC; 2009. p. 715.

Hasanudin K, Hashim P, Mustafa S. Corn silk (Stigma maydis) in healthcare: A phytochemical and pharmacological review. Molecules 2012;17:9697-715.

Published

2018-04-26

How to Cite

Pardede, T. R., and M. Muchlisyam. “ANALYSIS ON CALCIUM SOLUBILITY IN KIDNEY STONES (IN VITRO) AND DIURETIC EFFECT (IN VIVO) USING CORN SILK (ZEA MAYS L.) INFUSE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 13, Apr. 2018, pp. 80-83, doi:10.22159/ajpcr.2018.v11s1.26573.

Issue

Section

Original Article(s)