APPLICATION AND VALIDATION OF DERIVATIVE SPECTROPHOTOMETRIC FOR DETERMINATION LEVELS OF TERNARY MIXTURES OF PARACETAMOL, PROPYPHENAZONE, AND CAFFEINE IN TABLET DOSAGE FORM
Keywords:Paracetamol, Propyphenazone, Caffeine, Derivative spectrophotometry, Zero-crossing, Phosphate buffer (pH 72), Methanol
Â Objective: This study was to develop a spectrophotometric method with derivative zero-crossing for determines the levels of paracetamol (PCT), propyphenazone (PRO), and caffeine (CAF) in tablet dosage form without prior separation.
Method: The study begins with optimizing the type of solvent, phosphate buffer (pH 7.2) and a mixture of phosphate buffer (pH 7.2) with methanol at ratio 90:10; 70:30; 50:50; 30:70; and 10:90. Spectrophotometric method with zero-crossing, tested validity based on linearity, accuracy, precision, limit of detection, and limit of quantification. Then, the method applied to determine the levels of PCT, PRO, and CAF in tablet.
Result: The mixture of phosphate buffer (pH 7.2) with methanol at ratio 70:30 can be used for analysis. Applications zero-crossing technique on assay of PCT and CAF performed on the first derivative and Î”Î» 2 with Î» 239.4 nm for PCT and Î”Î» 8 with Î» 245.6 nm for CAF while PRO in the third derivative and Î”Î» 8 with Î» 249.6 nm, resulting 100.91%, 104.75%, and 103.33% for levels of PCT, PRO, and CAF, respectively.
Conclusion: Spectrophotometric derivative method with zero-crossing qualified in validation parameters.
Vichare V, Mujgond P, Tambe V, Dhole SN. Simultaneous Spectrophotometric determination of paracetamol and caffeine in tablet formulation. Int J Pharm Tech Res 2010;2:2512-6.
Delvadiya K, Ritu K, Prachi K, Sunil K, Pratik P. Spectrophotometric simultaneous analysis of paracetamol, propyphenazone and caffeine in tablet dosage forms. Int J Pharm Pharm Sci 2011;3:170.
Adupa S, Sr A, Simhadri H, Kalidindi SV. Simultaneous estimation of paracetamol caffiene and propyphenazone in bulk and pharmaceutical dosage form by RP-HPLC. J Pharm Res 2014;8:331-5.
Soponar F, Staniloae D, Moise G, Szaniszlo B, David V. Simultan determination of paracetamol, propyphenazone and caffeine from pharmaceutical preparation in the presence of related substances using a validated HPLC â€“ DAD method. Rev Roum Chim 2013;58:433-40.
El-Sayed AA, El-Salem NA. Recent development of derivative spectrophotometry and their analytical applications. Anal Sci 2005;21:595-614.
Ã–zgÃ¼r MÃœ, Ikbal K. Determination of ternary mixtures of vitamins (B1, B6, B12) by zero-crossing derivative spectrophotometry. Turk J Chem 2002;26:385-91.
Saraan SM, Sinaga SM, Muchlisyam. Development method for determination of ternary mixture of paracetamol, ibuprofen and caffeine in tablet dosage form using zero-crossing derivative spectrophotometric. Int J Pharm Tech Res 2015;7:349-53.
Health Department of Republic of Indonesia. Indonesian Pharmacopoeia. 4th ed. Indonesian: General Directory of Drug and Food Control; 1995.
DinÃ§ E, KÃ¶kdil G, Onur F. Derivative ratio spectra-zero crossing spectrophotometry and LC method applied to the quantitative determination of paracetamol, propyphenazone and caffeine in ternary mixtures. J Pharm Biomed Anal 2001;26:769-78.
Hajian R, Afshari N. The spectrophotometric multicomponent analysis of ternary mixture of ibuprofen, caffeine and parasetamol by the combination of double divisor-ratio spectra derivative and h-point standard addition method. E J Chem 2012;9:1154-5.
Abdel-Hay M, Azza AG, Ekran MH, Tarek SB. Derivative and derivative ratio spectrophotometric analysis of antihypertensive ternary mixture of amiloride hydrochloride, hydrochlorthiazide and timolol maleate. J Chin Chem Soc 2008;55:971-8.
Nejal MB, Chavada VD, Sanyal M, Shrivastav PS. Manipulating ratio spectra for the spectrophotometric analysis of diclofenac sodium and pantoprazole sodium in laboratory mixtures and tablet formulation. Sci World J 2014;2014:1-10.
PopoviÄ‡ GV, Lidija BP, Violeta MS. Analytical application of derivative spectrophotometry. J Serb Chem Soc 1999;65:464.
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