APPLICATION AND VALIDATION OF DERIVATIVE SPECTROPHOTOMETRIC FOR DETERMINATION LEVELS OF TERNARY MIXTURES OF PARACETAMOL, PROPYPHENAZONE, AND CAFFEINE IN TABLET DOSAGE FORM

Authors

  • Artha Yuliana Sianipar Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Muchlisyam Muchlisyam Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Siti Morin Sinaga Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26553

Keywords:

Paracetamol, Propyphenazone, Caffeine, Derivative spectrophotometry, Zero-crossing, Phosphate buffer (pH 72), Methanol

Abstract

 Objective: This study was to develop a spectrophotometric method with derivative zero-crossing for determines the levels of paracetamol (PCT), propyphenazone (PRO), and caffeine (CAF) in tablet dosage form without prior separation.

Method: The study begins with optimizing the type of solvent, phosphate buffer (pH 7.2) and a mixture of phosphate buffer (pH 7.2) with methanol at ratio 90:10; 70:30; 50:50; 30:70; and 10:90. Spectrophotometric method with zero-crossing, tested validity based on linearity, accuracy, precision, limit of detection, and limit of quantification. Then, the method applied to determine the levels of PCT, PRO, and CAF in tablet.

Result: The mixture of phosphate buffer (pH 7.2) with methanol at ratio 70:30 can be used for analysis. Applications zero-crossing technique on assay of PCT and CAF performed on the first derivative and Δλ 2 with λ 239.4 nm for PCT and Δλ 8 with λ 245.6 nm for CAF while PRO in the third derivative and Δλ 8 with λ 249.6 nm, resulting 100.91%, 104.75%, and 103.33% for levels of PCT, PRO, and CAF, respectively.

Conclusion: Spectrophotometric derivative method with zero-crossing qualified in validation parameters.

References

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Published

2018-04-26

How to Cite

Sianipar, A. Y., M. Muchlisyam, and S. Morin Sinaga. “APPLICATION AND VALIDATION OF DERIVATIVE SPECTROPHOTOMETRIC FOR DETERMINATION LEVELS OF TERNARY MIXTURES OF PARACETAMOL, PROPYPHENAZONE, AND CAFFEINE IN TABLET DOSAGE FORM”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 13, Apr. 2018, pp. 8-11, doi:10.22159/ajpcr.2018.v11s1.26553.

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