ANTIOXIDANT ASSAY OF PHASEOLUS VULGARIS L BEANS ETHANOLIC EXTRACT IN VITRO AND IN VIVO
Keywords:Antioxidant, Phaseoleus vulgaris L, Beans, Ethanolic extract, In vitro, In vivo
Objectives: Antioxidant is a substance such as Vitamin C that able to remove potentially damaging oxidizing agents. Malondialdehyde (MDA) is an important marker of oxidative stress that have correlation with Type 2 diabetes mellitus (DM) progression. Phaseolus vulgaris L (PV) (beans) is one of the alternative medicines to treat DM in Indonesia. We investigated the antioxidant activity of ethanolic extract on PV (EEPV) beans using in vitro and in vivo model.
Methods: Antioxidant assay in vitro was conducted using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Alloxan (120 mg/kg, intraperitoneally)- induced diabetic rats were divided into 5 groups (n=5), i.e., NC: Normal control, P1: Diabetic-control, P2, P3, and P4 (200 mg/kg, 400 mg/kg, and 600 mg/kg of EEPV, orally) for 28 days. MDA serum level was evaluated using spectrophotometer. The data were analyzed by one-way ANOVA test.
Results: The antioxidant assay in vitro showed that EEPV has very weak (IC50=641.87 Î¼g/ml, whereas Vitamin C as standard showed very strong activity (IC50=2.95 Î¼g/ml). MDA level were 2.80Â±0.09 nmol/ml (NC), 3.30Â±0.42 nmol/ml (P1), 3.27Â±4.23 nmol/ml (P2), 2.93Â±0.05 nmol/ml (P3), and 3.17Â±0.25 nmol/ml (P4). There was no significant differences among normal control, diabetic-control, and EEPV-treated groups (p>0.05).
Conclusion: EEPV showed a very weak antioxidant activity in vitro.
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