MODIFICATED EXTRACTION AND PURITY TEST OF ARENGA PINNATA GUM
Keywords:Galactomannan, Palm seed, Arenga pinnata, Modification, Extraction, Luff Schoorl
Objective: The purpose of this study is modificated extraction method to obtain gum from Arenga pinnata Merr. and their purity test.
Methods: Gum has been extracted from palm seed using distilled water by centrifugation and warming. Characterization of gum was done by scanning electron microscopy-energy dispersivse X-ray and spectrophotometer infrared. Gum refluxed with HCl 2 N for 14 h would be the monomer. Galactose and mannose were used as standard. Purity test was done by the thin-layer chromatography (TLC) and Luff Schoorl method.
Results: The study results obtained that the modification methods (4.8%) and centrifugation (4.6%). Identification of functional groups with the spectrophotometer infrared provided the same spectrum forms between the two methods. Purity test by TLC with the best eluen butanol: ethanol:water 2:2:2. Luff Schoorl's method gives results of 87.46%, whereas the raw comparison galactose-mannose standard 96.69%.
Conclusions: The modification method gives more results with the same purity rate as the centrifugation method.
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