• Jamaran Kaban Department of Chemistry, Universitas Sumatera Utara, Medan, Indonesia.
  • Julia Reveny Department of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Juliati Tarigan Department of Chemistry, Universitas Sumatera Utara, Medan, Indonesia.
  • Nilsya Febrika Zebua Department of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.



Galactomannan, Palm seed, Arenga pinnata, Modification, Extraction, Luff Schoorl


Objective: The purpose of this study is modificated extraction method to obtain gum from Arenga pinnata Merr. and their purity test.

Methods: Gum has been extracted from palm seed using distilled water by centrifugation and warming. Characterization of gum was done by scanning electron microscopy-energy dispersivse X-ray and spectrophotometer infrared. Gum refluxed with HCl 2 N for 14 h would be the monomer. Galactose and mannose were used as standard. Purity test was done by the thin-layer chromatography (TLC) and Luff Schoorl method.

Results: The study results obtained that the modification methods (4.8%) and centrifugation (4.6%). Identification of functional groups with the spectrophotometer infrared provided the same spectrum forms between the two methods. Purity test by TLC with the best eluen butanol: ethanol:water 2:2:2. Luff Schoorl's method gives results of 87.46%, whereas the raw comparison galactose-mannose standard 96.69%.

Conclusions: The modification method gives more results with the same purity rate as the centrifugation method.


Tarigan J, Purba J. The characterization of phosphate crosslinked galactomannan kolang kaling†polysaccharide. Majalah Polim Indones 2015;18:1-8.

Kooiman P. Structures of the galactomannans from seeds of Annona muricata, Arenga saccharifera, Cocos nucifera, Convolvulus tricolor, and Sophora japonica. Carbohydr Res 1971;20:329-37.

Tamaki Y, Teruya T, Tako M. The chemical structure of galactomannan isolated from seed of Delonix regia. J Biosci Biotechnol Biochem 2010;74:1110-2.

Mathur V, Mathur NK. Fenugreek and other lesser known legume galactomannan-polysaccharides: Scope for developments. J Sci Ind Res 2005;64:475-81.

Cerqueira MA, Bourbon AI, Pinheiro AC, Martins JT, Souza BW, Teixeria AJ, et al. Galactomannans Use in the development of the edible films/coatings for food applications. Food Sci Technol 2011;22:662-71.

Kabir GI, Yagen B, Penhasi A, Rubinstein A. Low swelling, crosslinked guar and its potential use as colon-specific drug carrier. Pharm Res 1998;15:1019-25.

Suryaningsiha NL, Pasaribu YP. Dewaka banana as an alternative energy source. Procedia Food Sci 2015;3:211-5.

Veerappa HM, Sirigeri JP. Investigating plant galactomannans. Biochem Mol Biol Educ 2002;30:101-3.

Silverstein RM, Bassler GG, Morrill TC. Spectrometric Identification of Organic Compounds. 7th ed. New York: John Wiley &Sons; 2005.



How to Cite

Kaban, J., J. Reveny, J. Tarigan, and N. F. Zebua. “MODIFICATED EXTRACTION AND PURITY TEST OF ARENGA PINNATA GUM”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 13, Apr. 2018, pp. 148-50, doi:10.22159/ajpcr.2018.v11s1.26593.



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