IDENTIFICATION OF BIOACTIVE COMPOUNDS IN CYMODOCEA SERRULATA-A SEAGRASS BY GAS CHROMATOGRAPHY–MASS SPECTROSCOPY

Authors

  • Pushpabharathi N Department of Biochemistry, Vels Institute of Science Technology and Advance Science, Pallavaram, Chennai – 600 039, Tamil Nadu, India.
  • Jayalakshmi M Department of Biochemistry, Vels Institute of Science Technology and Advance Science, Pallavaram, Chennai – 600 039, Tamil Nadu, India.
  • Amudha P Department of Biochemistry, Vels Institute of Science Technology and Advance Science, Pallavaram, Chennai – 600 039, Tamil Nadu, India.
  • Vanitha V Department of Biochemistry, Vels Institute of Science Technology and Advance Science, Pallavaram, Chennai – 600 039, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i9.26744

Keywords:

Nil, Cymodocea serrulata, Seagrass, Palmitic acid, Phytocompounds

Abstract

Objective: The objective of this study was to identify the lead phytocompounds present in the ethanol extract of the seagrass Cymodocea serrulata by gas chromatography–mass spectrometry (GCMS).

Methods: 1 kg of C. serrulata whole seagrass powder was subjected to extraction on polarity basis using five solvent such as hexane, chloroform, ethyl acetate, ethanol, and water. Since ethanol extract showed a maximum antioxidant property, its phytochemicals were investigated using GCMS technique. The phytocompounds identified through GC were interpreted with mass spectra national institute standard and technology library.

Result: The GCMS analysis of ethanol extract of C. serrulata identified peaks of six different compounds they are hexahydrofarnesyl acetone (7.70%), hexadecanoic acid, methyl ester (4.11%), tetradecanoic acid (62.89%), pentadecanoic acid(62.89%), cholesta4, 6dien3ol (5.88%), and stigmasterol (19.42%).

Conclusion: The GCMS study of C. serrulata ethanol extract unveils the presence of bioactive compounds that have a pharmacological and nutraceutical values.

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Author Biographies

Pushpabharathi N, Department of Biochemistry, Vels Institute of Science Technology and Advance Science, Pallavaram, Chennai – 600 039, Tamil Nadu, India.

DEPARTMENT OF BIOCHEMISTRY AND RESEARCH SCHOLAR

Jayalakshmi M, Department of Biochemistry, Vels Institute of Science Technology and Advance Science, Pallavaram, Chennai – 600 039, Tamil Nadu, India.

DEPARTMENT OF BIOCHEMISTRY AND RESEARCH SCHOLAR

Amudha P, Department of Biochemistry, Vels Institute of Science Technology and Advance Science, Pallavaram, Chennai – 600 039, Tamil Nadu, India.

department of biochemistry and research scholar

Vanitha V, Department of Biochemistry, Vels Institute of Science Technology and Advance Science, Pallavaram, Chennai – 600 039, Tamil Nadu, India.

department of biochemistry and head and associate professor

References

Lee SH, Jeon YJ. Anti-diabetic effects of brown algae-derived phlorotannins, marine polyphenols through diverse mechanisms. J Fitoterapia 2013;86:129-36.

Harrison PG. Control of microbial growth and of amphipod grazing by water-soluble compounds from leaves of Zostera marina. Mar Biol 1982;67:225-30.

Bharathi NP, Amudha P, Vanitha V. Sea grasses-novel marine nutraceuticals. Int J Pharm Bio Sci 2016;7:567-73.

Hardoko, Primaoktasa D, Yuli E. Anticancer potential of Sea grass leaves Cymodecea serrulata CRUDE extract on HeLa cell. J Chem Pharm Res 2016;8:571-6.

Papitha R, Ravi L, Selvaraj CI. Phytochemical studies and GCMS analysis of Spermadictyon suaveolens roxb. Int J Pharm Pharm Sci 2017;9:143-9.

Kaur N, Chaudhary J, Jain A, Kishore L. Stigmasterol: A comprehensive review. Int J Pharm Sci Res 2011;2:2259-65.

Sayık A, Yusufoğlu AS, Acık L, Arslan L. DNA-binding, biological activities, and chemical composition of wild growing Epilobium angustifolium L. Extracts from Canakkale, Turkey. J Turkish Chem Soc A 2017;4:811-40.

Shin WS, Oh S, An SW, Park GM, Kwon D, Ham J, et al. 5E- and 5Z-farnesylacetones from Sargassum siliquastrum as novel selective L-type calcium channel blockers. J Vasc Pharmacol 2013;58:299-306.

Dabadie H, Peuchant E, Bernard M, LeRuyet P, Mendy F. Moderate intake of myristic acid in sn-2 position has beneficial lipidic effects and enhances DHA of cholesteryl esters in an interventional study. J NutrBiochem 2005;16:375-82.

Selvamangai1 C, Bhaskar A. GC-MS analysis of phytocomponents in the methanolic extract of eupatorium triplinerve. Int J Drug Dev Res 2012;4:148-53.

Warensjö E, Jansson JH, Cederholm T, Boman K, Eliasson M, Hallmans G, et al. Biomarkers of milk fat and the risk of myocardial infarction in men and women: A prospective, matched case-control study. Am J Clin Nutr 2010;92:194-202.

Sharma D, Rani R, Chaturvedi M, Yadav JP. Antibacterial capacity and identification of bioactive compounds by GCMS of Allium cepa. Int J Pharm Pharm Sci 2018;10:116-21.

Schmelzle H, Wirth S, Skopnik H, Radke M, Knol J, Böckler HM, et al. Randomized double-blind study of the nutritional efficacy and bifidogenicity of a new infant formula containing partially hydrolyzed protein, a high beta-palmitic acid level, and nondigestible oligosaccharides. J Pediatr Gastroenterol Nutr 2003;36:343-51.

Okoh O, Grosspietzsch R, von Klitzing L. Is the intake of palm oil (palmitic acid) in meals associated with the low incidence of respiratory distress syndrome in Nigeria? (author’s transl). Monatsschr Kinderheilkd 1979;127:669-74.

Zhang L, Ji J, Zhu XY, Wu YY, Yu H, Zhang B, et al. Palmitic acid induces apoptosis in human hepatoma cell line, HepG2 cells. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2004;26:671-6.

William Still Well. An Introduction to Biological Membranes Composition, Structure and Function. 2nd ed. London: Elseiver: Academic Press; 2016. p. 89-110.

Dorr P. Therapeutic Areas II: Cancer, Infectious Diseases, Inflammation And immunology and dermatology comprehensive medicinal chemistry II. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. USA: Elsevier; 2007. p. 419-43.

Golley RK, Hendrie GA. Evaluation of the relative concentration of serum fatty acids C14:0, C15:0 and C17:0 as markers of children’s dairy fat intake. Ann Nutr Metab 2014;65:310-6.

Arunkumar R, Nair SA, Subramoniam A. Induction of cell-specific apoptosis and protection of mice from cancer challenge by a steroid positive compound from Zornia diphylla (L.) Pers. J Pharmacol Pharmacother 2012;3:233-4.

Kametani T, Furuyama H. Synthesis of vitamin D3 and related compounds. Med Res Rev 1987;7:147-71.

Batta AK, Xuab G, Honda A, Miyazaki T, Salen G. Stigmasterol reduces plasma cholesterol levels and inhibits hepatic synthesis and intestinal absorption in the rat. J Pharm Sci 2006;55:292-9.

Shin WS, Oh S, An SW, Park GM, Kwon D, Ham J, et al. 5E- and 5Z-farnesylacetones from Sargassum siliquastrum as novel selective L-type calcium channel blockers. Vascul Pharmacol 2013;58:299-306.

Published

07-09-2018

How to Cite

N, P., J. M, A. P, and V. V. “IDENTIFICATION OF BIOACTIVE COMPOUNDS IN CYMODOCEA SERRULATA-A SEAGRASS BY GAS CHROMATOGRAPHY–MASS SPECTROSCOPY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 9, Sept. 2018, pp. 317-20, doi:10.22159/ajpcr.2018.v11i9.26744.

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