EVALUATION OF NEUROPHARMACOLOGICAL ACTIVITY OF MEDICINAL PLANT
Objective: The objective of the study is to evaluate the antiparkinsonian activity of hydroalcoholic extract of the seeds of Canavalia gladiata (HECG) in zebrafish and Swiss albino mice.
Materials and Methods: Catalepsy was induced in zebrafish by exposing them to haloperidol solution. Treatment groups were exposed to bromocriptine and HECG, 30 min before haloperidol exposure at the dose of 2, 5, and 10 Î¼g/mL. Latency to travel from one fixed point to another, time spent near the bottom of the tank, and complete cataleptic time were evaluated to assess behavioral changes. In mice, catalepsy was induced using haloperidol (1 mg/kg i.p.). Treatment groups received bromocriptine (2.5 mg/kg) and HECG at the dose of (100, 200, and 300 mg/kg) orally. Bar test for catalepsy, motor coordination test by rotarod, and locomotor activity by actophotometer were carried out to assess behavioral changes.
Results: Bromocriptine and HECG-treated groups showed significant difference in behavioral parameters as compared to haloperidol control group in both the experimental models.
Conclusion: Canavalia gladiata seeds exhibited significant antiparkinsonian activity in haloperidol mouse model and zebrafish. Zebrafish can be used with ease and effectiveness for initial screening of drugs before subjecting them to rodent testing.
2. Suvarna P, Ingale SBK. Antioxidant and antiparkinsonian activity of Passiflora incarnate leaves. Orient Pharm Exp Med 2014;14:231-6.
3. Flinn L, Bretaud S, Lo C, Ingham PW, Bandmann O. Zebrafish as a new animal model for movement disorders. J Neurochem 2008;106:1991-7.
4. Giacomini NJ, Rose B, Kobayashi K, Guo S. Antipsychotics produce locomotor impairment in larval zebrafish. Neurotoxicol Teratol 2006;28:245-50.
5. Guo, S., Using zebrafish to assess the impact of drugs on neural development and function. Expert Opin Drug Discov 2009;4:715-26.
6. Storer NY, Zon LI. Zebrafish model of P53 function. Old Spring Harb Perspect Biol 2010;2:a001123.
7. Dakshayini PN, Piler B, Boob M. Phytochemical screening and in vitro antioxidant potential of Tribulus terrestris fruit and Mesuaferrea flower extracts: A comparative study. Int J Pharm Pharm Sci 2018;10:70.
8. Khandelwal K.R. Practical Pharmacognosy, Techniques and Experiments. 6th ed. Pune: Nirali Prakashan; 2014.
9. Kokate CK, Purohit AP. Pharmacognosy. 34th ed. Pune: Nirali Prakashan Publication; 2003.
10. Makhija DT, Jagtap AG. Studies on sensitivity of zebrafish as a model organism for Parkinsonâ€™s disease: Comparison with rat model. J Pharmacol Pharmacother 2014;5:39-46.
11. Bishnoi M, Chopra K, Kulkarni SK. Involvement of adenosinergic receptor system in an animal model of tardive dyskinesia and associated behavioural, biochemical and neurochemical changes. Eur J Pharmacol 2006;552:55-66.
12. Kulkarni SK. Hand book of Experimental Pharmacology. New Delhi: Vallabh Prakashan; 1987.
13. Gurpreet S, Nisha R, Kirti S, Amita S, Sinha V. Investigating the potential of an antidepressant intranasal mucoadhesive microemulsion. Int J Pharm Pharm Sci 2018;10:125.
14. Goyal RK. Practical in Pharmacology. 5th ed. Ahmedabad: B.S. ShahPrakashan; 2005.
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