COMPARATIVE ANALYSIS OF MORPHOLOGY AND PHYTOCHEMICAL CONSTITUENTS IN DIFFERENT POPULATIONS AND MORPHOTYPES OF DATURA INNOXIA MILL. AND DATURA METEL L. FROM PUNJAB PLAINS

  • Mahinder Partap Department of Botany, Punjabi University, Patiala, Punjab, India.
  • Raghbir Chand Gupta Department of Botany, Punjabi University, Patiala, Punjab, India.
  • Saroj Kumar Pradhan Department of Botany, Punjabi University, Patiala, Punjab, India.

Abstract

Objective: Comparative analysis of morphology and phytochemical constituents in different populations and morphotypes of Datura innoxia Mill. and Datura metel L. from Punjab plains.

Methods: Morphological analyses of different populations have been done. Methanol extracts of seeds and roots of different populations have been prepared and total phenols, flavonoid contents were measured through spectrophotometry. Antioxidant activity was studied by 2, 2-diphenyl-1- picrylhydrazyl radical scavenging activity and total antioxidant capacity. Two major compounds, caffeic acid and chlorogenic acid were quantified by high-performance thin-layer chromatography (HPTLC) analyses.

Results: Two morphotypes of D. metel were reported in the present study. Out of five different populations of D. metel and D. innoxia, the wild populations have more bioactive compound as compared to the cultivated ones from the detailed phytochemical investigation. Pharmacologically important two marker compounds chlorogenic acid and caffeic acid has been identified and quantified by HPTLC technique.

Conclusion: Variation in terms of morphology and secondary metabolites exists among the different populations of Datura spp. Among the two plant parts studied, seeds have the maximum amount of bioactive metabolites and antioxidant activity. This study revealed that chlorogenic acid and caffeic acid are the potential polyphenolic compounds in Datura spp. It has been found that the antioxidant activity of plant is due to its polyphenol contents, which provides insight to various researchers to work on it as it imparts health benefit.

Keywords: Datura metel, Datura innoxia, Morphotype, Phytochemical analysis, High-performance thin-layer chromatography.

References

1. Dorman HJ, Deans SG. Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. J Appl Microbiol 2002;88:308-16.
2. Preissel U, Preissel HG. Brugmansia and Datura: Angel’s Trumpets and Thorn Apples. Buffalo, New York: Firefly Books; 2002. p. 106-29.
3. Dogra KS, Kohli RK, Sood SK. An assessment and impact of three invasive species in the Shivalik hills of Himachal Pradesh, India. Int J Biodivers Conserv 2009;1:4-10.
4. Nandini D, Ravikumar BS, Rashmi KK. Morphometric analysis of Datura plant to understand variation and similarities among four major species. Med Aromat Plants 2015;4:4.
5. Harbourne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. London: Chapman and Hall Ltd.; 1973. p. 279.
6. Okwu DE. Phytochemical and vitamin content of indigenous spices of South Eastern Nigeria. J Sustain Agric Environ 2004;6:30-34.
7. Boerjan W, Ralph J, Baucher M. Lignin biosynthesis. Annu Rev Plant Biol 2003;54:519-46.
8. Olthof MR, Hollman PC, Katan MB. Chlorogenic acid and caffeic acid are absorbed in humans. J Nutr 2001;131:66-71.
9. Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Vitic 1977;28:49-55.
10. Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med 2012;12:221.
11. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of Vitamin E. Anal Biochem 1999;269:337-41.
12. Otang WM, Grierson DS, Ndip RN. Antifungal activity of Arctotis arctotoides (L.f.) O. Hoffm. and Gasteria bicolor Haw. Against opportunistic fungi associated with HIV/AIDS. Pharmacog Mag 2012;30:135-40.
13. Pradhan SK, Gupta RC, Goel R, Preet R. Simultaneous determination of chlorogenic and caffeic acid in Siegesbeckia orientalis L.(Xi Xian) by a validated high-performance thin-layer chromatographic method. J Planar Chromatogr 2017;30:516-20.
14. Joy PP, Thomas J, Mathew S, Skaria, B.P. Medicinal Plants. Tropical Horticulture vol. 2. (eds. Bose, T.K., Kabir, J., Das, P. and Joy, P.P.). Naya Prokash, Calcutta 2001: 449-632.
15. Abdelkader M, Arezki D, Khelifi-Slaoui’s M, Khelifi L, Amdoun R, Bakiris N. Morphological diversity and hyoscyamine/scopolamine contents in twelve Algerian samples of Datura stramonium L. of different origin. Rev Ecol 2011;66:291-302.
16. Hossain MA, Nagooru MR. Biochemical profiling and total flavonoids contents of leaves crude extract of endemic medicinal plant Corydyline terminalis L. Kunth. Pharmacogn J 2011;3:25-9.
17. Suresh SN, Nagarajan N. Preliminary phytochemical and antimicrobial activity analysis of Begonia malabarica Lam. J Basic Appl Biol 2009;3:59-61.
18. Gonzalez-Guevara JL, Gonzalez-Lavaut JA, Pino-Rodriguez S, Garcia-Torres M, Carballo-Gonzalez MT, Echemendia-Arana OA, et al. Phytochemical screening and in-vitro antiherpetic activity of four Erythtroxylum species. Acta Farm Bonaer 2004;23:506-9.
19. Jamdhade MS, Survase SA, Kare MA, Bhuktar AS. Phytochemical studies on Datura metel L.in Marathwada region, Maharashtra. J Phytol 2010;2:46-8.
20. Mahomoodally MF, Gurib-Fakim A, Subratty AH. Antimicrobial activities and phytochemical profiles of endemic medicinal plants of Mauritius. Pharm Biol 2008;43:237-42.
21. Gulcin I, Haci AA, Cesur M. Determination of in-vitro antioxidant and radical scavenging activities of propofol. Chem Pharm Bull 2005;53:281-5.
22. Barile E, Bonanomi G, Antignani V, Zolfaghari B, Ebrahim SS, Scala F, et al. Phytochemical screening and antimicrobial assessment of Abutilon mauritianum, Bacopa monifera and Datura stramonium. Phytochemistry 2007;68:596-603.
23. Ayoola GA, Coker HA, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, et al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in South Western Nigeria. Trop J Pharm Res 2008;7:1019-24.
24. Akharaiyi FC. Antibacterial, phytochemical and antioxidant activities of Datura metel. Int J Pharm Tech Res 2011;3:478-83.
25. Varahalarao V, Kaladhar DS. Antimicrobial study of plant extracts of Datura metel L. against some important disease causing pathogens. Asian Pac J Trop Dis 2012;2:94-9.
26. Sekar D, Kolanjinathan K, Saranraj P, Gajendiran K. Screening of Phyllanthus amarus, Acalypha indica and Datura metel for its antimicrobial activity against selected pathogens. Int J Pharm Biol Sci Arch 2012;3:1231-5.
27. Waza SA, Anthony P, Dar S. Phytochemical analysis, antioxidant and antimicrobial activities of methanolic extract of Datura stramonium seeds. Int J Pharm Sci Res 2015;6:3021-6.
28. Anyasor GN, Ogunwenmo KO, Oyelana OA, Akpofunure BE. Phytochemical constituents and antioxidant activities of aqueous and methanol stem extracts of Costus afer Ker Gawl. (Costaceae). Afr J Biotechnol 2010;9:4880-4.
29. Chao PD, Hsiu SL, Hou YC. Flavonoids in herbs: Biological fates and potential interactions with xenobiotics. J Food Drug Anal 2002;10:219-28.
30. Igbinosa OO, Igbinosa EO, Aiyegoro OA. Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). Afr J Pharm Pharmacol 2009;3:58-62.
31. Thitilertdecha N, Teerawutgulrag A, Rakariyatham N. Antioxidant and antibacterial activities of Nephelium lappaceum L. extracts. Food Sci Technol 2008;41:2029-35.
32. Hollman PC, Katan MB. Dietry flavonoids: Intake, health effects and bioavailability. Food Chem Toxicol 1999;37:937-42.
33. Chidambaram V, Niraimathi L, Sudha V, Lavanya R, Vadivel V, Brindha P. Spectrophotometric, HPTLC and GC-MS studies on selected spice extracts. Int J Pharm Pharm Sci 2015;7:184-90.
34. Jaafar NS, Hamad MN, Abbas IS, Jaafar IS. Qualitative phytochemical comparison between flavonoids and phenolic acids contents of leaves and fruits of Melia azedarach (family: Meliaceae) cultivated in Iraq by HPLC and HPTLC. Int J Pharm Pharm Sci 2016;8:242-50.
35. Usman MR, Surekha S, Rashmi S, Lalit S, Kumar N, Gadgoli J, et al. High performance thin layer chromatograpic method for quantification of β-sitosterol from Vanda roxburghii R.Br. Asian J Plant Sci Res 2012;4:113-24.
36. Pradhan SK, Gupta RC, Goel R. Differential content of secondary metabolites in diploid and tetraploid cytotypes of Siegesbeckia orientalis L. Nat Prod Res 2018;15:1-7.
37. Gopal V, Mandal SC. HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubils. J Acute Dis 2014;5:59-61.
38. Hussain MS, Fareed S, Ali M, Alam MS, Rahman MA, Srivastava AK. Phytochemical investigation and simultaneous estimation of bioactive lupeol and stigmasterol in Abutilon indicum by validation HPTLC method. J Coast Life Med 2014;2:394-401.
39. Rahmoune B, Zakarya I, Morsli A1, Khelifi-slaoui M, Khelifi L, Do amarante L. Phenylpropanoids and fatty acids levels in roots and leaves of Datura stramonium and Datura innoxia. Int J Pharm Pharm Sci 2017;9:150-4.
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How to Cite
Partap, M., R. C. Gupta, and S. K. Pradhan. “COMPARATIVE ANALYSIS OF MORPHOLOGY AND PHYTOCHEMICAL CONSTITUENTS IN DIFFERENT POPULATIONS AND MORPHOTYPES OF DATURA INNOXIA MILL. AND DATURA METEL L. FROM PUNJAB PLAINS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 193-9, doi:10.22159/ajpcr.2019.v12i1.27720.
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