QUANTITATIVE PHYTOCHEMICAL SCREENING, THIN-LAYER CHROMATOGRAPHY ANALYSIS, HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FINGERPRINTING, AND ANTIOXIDANT ACTIVITY OF LEAVES OF DIOSPYROS MONTANA (ROXB.)
Objective: The objective of this study was to investigate important phytochemical constituents and antioxidant potential of Diospyros montana Roxb. leaves belonging to the family Ebenaceae.
Methods: Leaves were exhaustively extracted with ethanol and fractionated into petroleum ether, chloroform, and ethyl acetate extracts. The various fractions were further analyzed for phytochemical composition and concentration-dependent antioxidant activity using conventional methods and high-performance thin-layer chromatography (HPTLC) fingerprinting. Since leaves contained phenolic compounds, extracts were evaluated for total phenolic content, flavonoids contents, and in-vitro antioxidant activity. Antioxidant potential was assessed using parameters such as superoxide radical scavenging, nitric oxide inhibition, and β-carotene/linoleic acid antioxidant activity.
Results: Primitive phytochemical investigation highlighted the presence of steroids, saponins, flavonoids, alkaloids, and tannins which were confirmed by TLC and HPTLC fingerprinting. The antioxidant activity of leaf extracts decreased in the following order ethyl acetate > ethanolic > chloroform > petroleum ether and it was comparable with standards such as ascorbic acid and butylated hydroxytoluene.
Conclusion: The present study concludes that the ethanolic extract and fractions of D. montana (Roxb.) leaves have prominent antioxidant activity comparable to standards. Therefore, D. montana (Roxb.) leaves may be used as a probable source of natural antioxidants in the pharmaceutical industry.
2. Mallavadhani UV, Panda AK, Rao YR. Pharmacology and chemotaxonomy of diospyros. Phytochemistry 1998;49:901-51.
3. Priya S, Nethaji S. Antipyretic activity of ethanolic extract of leaf and bark of Diospyros Virginiana in yeast induced pyrexia. Int J Pharm Pharm Sci 2015;7:502-4.
4. Puri AV. Microscopical evaluation, phytochemical screening, and high performance thin layer chromatography fingerprinting of leaves of Diospyros montana (Roxb.). Int J Green Pharm 2018;12:205-11.
5. Maridass M, Ghanthikumar S, Raju G. Phytochemical analysis of Diospyros species. Ethnobot Lealf 2008;12:868-72.
6. Hazra B, Pal S, Banerjeet A, Ray R, Bhattacharya DK. Pharmacological studies on the effect of the treatment of Swiss a mice with diospyrin, a tumor-inhibitory plant product, and its synthetic derivatives. Phytother Res 1996;10:393-7.
7. Archana D, Dixitha M, Santhy K. Antioxidant and anti-clastogenic potential of Piper longum L. Int J Appl Pharm 2015;7:11-4.
8. Singh H, Dixit A, Sharma R, Sharma A. Comparative evaluation of total phenolic content, total flavonoid content and DPPH free radical scavenging activity of different plant parts of Vitex negundo. Int J Pharm Pharm Sci 2014;7:144-7.
9. Maxwell SR. Prospects for the use of antioxidant therapies. Drugs 1995;49:345-61.
10. Santharam B, Ganesh P, Soranam R, Divya VV, Lekshmi P. Free radical scavenging activity of various extracts of whole plant of Calycopteris floribunda (Lam.): An in-vitro evaluation. Int J Pharm Pharm Sci 2015;8:360-3.
11. Hemnani T, Parihar MS. Reactive oxygen species and oxidative DNA damage. Indian J Physiol Pharmacol 1998;42:440-52.
12. Halliwell B. Drug antioxidant effects. A basis for drug selection? Drugs 1991;42:569-605.
13. Wolfe K, Wu X, Liu RH. Antioxidant activity of apple peels. J Agric Food Chem 2003;51:609-14.
14. Basile A, Giordano S, López-Sáez JA, Cobianchi RC. Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry 1999;52:1479-82.
15. De Flora S. Problems and prospects in antimutagenesis and anticarcinogenesis. Mutat Res 1988;202:279-83.
16. Ahmad A, Husain A, Mujeeb M, Khan SA, Alhadrami HA, Bhandari A. Quantification of total phenol, flavonoid content and pharmacognostical evaluation including HPTLC fingerprinting for the standardization of Piper nigrum Linn fruits. Asian Pac J Trop Biomed 2015;5:101-7.
17. Sharma D, Bhatia V, Patil S, Sharma PC. Antimicrobial activity of selected cryptogams from solan region. Int J Biol Pharm Res 2013;4:448-54.Moffat CA. Clarke’s Analysis of Drugs and Poisons. London: Pharmaceutical Press; 2001. p. 392.
18. Atta AH, Mohamed NH, Nasr SM, Mounair SM. Phytochemical and pharmacological studies on Convolvulus fatmensis Ktze. J Natl Remedies 2007;7:109-19.
19. Shah CS, Qudry JS. A Text Book of Pharmacognosy. Ahmedabad: BS Shah Prakashan; 1989-90. p. 62-152.
20. Bruneton J. Pharmacognosy, Phytochemistry and Medicinal Plants. 2nd ed. London: Intercept Ltd.; 1999. p. 310-54.
21. Patel JD, Kumar V. Annona squamosa L. Phytochemical analysis and antimicrobial screening. J Pharm Res 2008;1:34-8.
22. Harborne JB. A Guide to Modern Techniques of Plant Analysis. In: Textbook of Phytochemical Methods. 5th ed. London: Chapman and Hall Ltd.; 1998. p. 91.
23. Wagner H, Baldt S. Plant Drug Analysis: A Thin Layer Chromatography Atlas. 2nd ed. Berlin: Springer; 1996. p. 196.
24. Sushma GS, Devi A, Madhulatha CH, Kumar U, Harathi P, Subramanian SN, et al. Preliminary phytochemical screening and HPTLC fingerprinting of leaf extracts of Ficus nervosa Heyne ex Roth. J Chem Pharm Res 2013;5:98-104.
5. Hossain MA, Nagooru MR. Biochemical profiling and total flavonoids contents of leaves crude extract of endemic medicinal plant Corydyline terminalis L. Kunth. Pharmacogn J 2011;3:25-30.
26. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.
27. Shreedhara CS, Aswatha RH, Zanvar SB, Gajera PF. Free radical scavenging activity of aqueous root extract of Argyreia nervosa (Burm.I.) BoJ. (Convolvulaceae). J Nat Remedies 2006;9:216-33.
28. Mangathayaru K, Sravan K, Reddy AK, Kumar RM, Swetha B, Reddy UC. In-vitro antioxidant studies of the aerial parts of Origanum majoram Linn. and Artemesia sieversiana Ehrh. Pharmacogn Mag 2007;3:90-4.
29. Habtemariam S, Jackson C. Antioxidant and cytoprotective activity of leaves of Peltiphyllum peltatum (Torr.) Engl Food Chem 2007;105:498 503.
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