THE ANTIOXIDANT STUDIES OF TWO MEDICINAL PLANTS, SPHAERANTHUS INDICUS AND PSOPHOCARPUS TETRAGONOLOBUS
Objective: The present study deals with the antioxidant assays of the different leaf extracts of two medicinal plants, Sphaeranthus indicus and Psophocarpus tetragonolobus.
Methods: Dried leaves of S. indicus and P. tetragonolobus were packed in separate round bottom flasks for sample extraction using ethanol, methanol, hexane, and distilled water as solvents for 72 h, and the extracts were collected after evaporating the solvents. Antioxidant studies of the various extracts were performed by 1-diphenyl-2-picrylhydrazyl and Ferric Reducing Ability of Plasma assays.
Results: Among the two plants studied, S. indicus showed better 2-diphenyl-1-picryl-hydrazyl (DPPH), scavenging activity than P. tetragonolobus with IC50 values of 174.380 and 262.313, respectively, as compared to that of the standard, ascorbic acid, IC50 value of which being 111.16. The FRAP assay results for both the plants indicated that the methanol fractions showed closer results when compared with standards, ascorbic acid and quercetin. The IC50 value of S. indicus, P tetragonolobus, ascorbic acid, and quercetin was 70.065, 151.953, 85.162, and 79.647, respectively. These results clearly indicate that S. indicus methanol fraction had better antioxidant activity when compared to both standards.
Conclusion: It is concluded that S. indicus and P. tetragonolobus have excellent antioxidant activities which could be the major contributing factors for their medicinal roles. Further studies in this direction are being carried on.
2. Rao MR, Anisha G. Preliminary phytochemical and GC MS study of one medicinal plant Carissa spinarum. Indo Am J Pharm Res 2018;8:414-21.
3. Rao MR, Balasubramaniam M. TLC, GC MS and antibacterial study of methanol extracts of Tribulus terrestris thorns and Morinaga oleifera flowers. Indo Am J Pharm Res 2018;5:3300-8.
4. Shil S, Rao MR, Prabhu K, Amuthvalli K. Thin layer chromatography - As a tool for standardization of ayurvedic medicine, trikatuchurna. Indo Am J Pharm Res 2018;5:5039-46.
5. Kotteswari M, Rao MR, Kumar S, Prabhu K, Sundaram RL, Dinakar S.. GC MS analysis of one ayurvedic preparation aswagandharishtam. Biomed Pharm J 2018;11:1061-72.
6. Donkor MN, Ayikanle SY, Adoesomdonkor S. Effect of ethanol stem bark extract of Berlina grandiflora Hutch and Dalz on marker liver enzymes in rats treated with. Int J Pharm Pharm Sci 2018;10:69-7.
7. Suwandi JF, Wijayanti MA. In vitro antiplasmodial and cytotoxic activities of a Sungkai (Peronema canescens) leaf extract. Int J Pharm Pharm Sci 2018;10:109-13.
8. Mahajan NG, Chopda MZ, Mahajan RT. A review on Sphaeranthus indicus Linn: Multipotential medicinal plant. Int J Pharm Res All Sci 2015;4:48-74.
9. Galani VJ, Patel BG, Rana DG. Sphaeranthus indicus Linn: A phytopharmacological review. Int J Ayrveda Res 2010;1:247-52.
10. Zachariah SM, Pappachen LK, Aneesh TP, Alex L, Sumith G, John MS, et al. Phytochemical and pharmacological screening of Sphaeranthus indicus Linn. For antimicrobial activity. Int J Pharm Sci Res 2010;1:169 73.
11. Nanda BK, Jena J, Rath B, Behera BR. Analgesic and antipyretic activity of whole parts of Sphaeranthus indicus Linn. J Chem Pharm Res 2009;1:207-12.
12. Nahata A, Saxena A, Suri N, Saxena AK, Dixit VK. Sphaeranthus indicus induces apoptosis through mitochondrial- dependent pathway in HL-60 cells and exerts cytotoxic potential on several human cancer cell lines. Integr Cancer Ther 2013;12:236-47.
13. Krishna TM, Thota SP, Jadhav M, Kamal KM, Venuganti A, Mrunalini D, et al. Studies on in vitro antioxidant and antibacterial activities of Sphaeranthus indicus (Linn). Int J Pharm Res Biomed Anal 2013;2:1-9.
14. Sundari K, Karthik D, Ilavenil S, Kaleeswaran B, Srigopalram S, Ravikumar S. Hepatoprotective and proteomic mechanism of Sphaeranthus indicus in paracetamol induced hepatotoxicity in Wistar rats. Food Biosci 2013;1:57-65.
15. Muhammad RT, Malik S, Ghulam M, Waseem H. Determination of the antidiabetic effect of methanolic extract of Sphaeranthus indicus L. On alloxan induced diabetic rabbits. Lat Am J Pharm 2011;30:378-82.
16. Meher BR, Mahar B, Rath BG, Sahoo SK. Antimicrobial activity of ethanolic extracts of leaves of Sphaeranthus indicus. Der Pharm Lett 2013;5:8-10.
17. Rao MR, Vijayalakshmi N. Preliminary phytochemical and GC MS analysis of different extracts of Sphaeranthus indicus leaves. Indo Am J Pharm Sci 2018;5:1511-20.
18. Lee KH, Padzil AM, Syahida A, Abdullah N, Zuhainis SW, Maziah M, et al. Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants. J Med Plants Res 2011;5:5555-63.
19. Sasidharan S, Zuraini Z, Yoga LL, Sangetha S, Suryani S. Antimicrobial activities of Psophocarpus tetragonolobus (L.) DC extracts. Food Borne Pathog Dis 2008;5:303-9.
20. Hamid R, Masood A, Wani IH, Rafiq S. Lectins: Proteins with diverse applications. J Appl Pharm Sci 2013;3 4 Suppl 1:S93-103.
21. Latha LY, Sasidharan S, Zuraini Z, Suryani S, Shirley L, Sangetha S, et al. Activity and toxicity of crude extract of the Psophocarpus tetragonolobus Pods. Afr J Trad Compl Alter Med 2007;4:59-63.
22. Rao MR, Lakshmi NV, Sundaram RL. Preliminary phytochemical and GC MS analysis of different extracts of Psophocarpus tetragonolobus leaves. Int Appl J Pharm Sci 2018;5:1649-56.
23. Solver-Rivas C, EspÃn JS, Wichers HJ. An easy and fast test to compare total free radical scavenger capacity of food stuffs. Phytochem Anal 2000;11:330-8.
24. Braca A, Sortino C, Politi M. Antioxidant activity of flavonoids from Licanialicaniaeflora. J Ethnopharm 2002;9:379-81.
25. Yen GC, Duh PD. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. J Agric Food Chem 1994;42:629-32.
26. Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr 1986;7:307-15.